真皮

Three antioxidative parameters(SOD super oxide dismutase、MDA malondialdehyde and GSH reduced glutathione hormone)were also measured.Results:The skin elasticity and water content improved increasingly according to the MPA measurements of the two group 4 weeks after the last irradiation.The hydroxyproline of the skin in the Q-switched 1064-nm Nd:YAG laser of 0.6J/cm~2、1.5 J/cm~2 and 2.5 J/cm~2 group showed 7.1%,13.1%and 19.8%(p＜0.05)increase respectively compared to their controls,the enhancement of dermal thickness were 15.5%,29.7%and 38.4%;The hydroxyproline of the skin in the Q-switched 532-nm Nd:YAG laser of 0.5J/cm~2、1.2 J/cm~2 and 2.0 J/cm~2 group showed 6.9%,9.6%and 14.2%(p＜0.05)increase respectively compared to their controls,the enhancement of dermal thickness were 5.5%,15.1%and 29.9%,among which the Q-switched 1064-nm laser group had the greatest effect. The result of antioxidative parameters showed some juvenescence change at the radiated skin.

结果：末次照射后第4周，Q开关1064-nmNd：YAG激光组(以下简称1064 nm组)和Q开关532-nmNd：YAG激光组(以下简称532-nm组)大鼠各实验侧的皮肤弹性和含水量都明显好于对照侧；1064-nm组和532-nm组实验侧的皮肤羟脯氨酸含量和真皮内胶原厚度都较对照组显著增大(P＜0.05)，其中1064-nm组的三种不同能量密度：0.6 J/cm~2、1.5 J/cm~2、2.5 J/cm~2的激光照射的皮肤区域的羟脯氨酸含量增加率为7.1%，13.1%和19.8%，真皮厚度增加率为15.5%，29.7%和38.4%，532-nm组的三种不同能量密度：0.5 J/cm~2、1.2 J/cm~2、2.0J/cm~2的激光照射的皮肤区域的羟脯氨酸含量增加率为6.9%，9.6%和14.2%，真皮层厚度增加率为5.5%，15.1%和29.9%，相比而言1064-nm组的羟脯氨酸及真皮层厚度增加率均较高；抗氧化指标显示两组大鼠实验侧皮肤的抗氧化能力增加，呈年轻化改变。

OBJECTIVE: To construct dermal template with dermal multipotent stem cells and to observe the survival state of grafts after transplantation.

目的：以真皮多能干细胞为种子细胞构建真皮再生模版，观察其移植后的成活状态。

Hair follicle dermal sheath housed multipotent and plastic stem cells, considered to be dermal stem cells niche.

毛囊真皮鞘包含具有可塑性的干细胞，认为是真皮干细胞的定居处。

Joint-stock restructuring of enterprises, changed its name to Qidong City Huayu leather Limited, is a multi-industry and trade as one entity enterprise, specializing in the production of leather fashion bags, leather cell phone bags, purses, briefcases, Oxford travelling bags, leisure Bao, schoolbags.

启东市华裕皮具有限公司创办于1982年，她的前称为启东市志良箱包厂、启东市箱包三厂。93年改制为股份制企业，更名为启东市华裕皮具有限公司，是一个以工贸为一体的多实体企业，专门生产真皮时装包、真皮手机包、钱包、公文包、牛津旅行包、休闲包、书包。

One of the many small, platelike dermal or epidermal structures that characteristically form the external covering of fishes, reptiles, and certain mammals.

真皮与表皮交界处凹凸不平，形成许多真皮乳头和表皮突一、表皮表皮主要由角朊细胞组成，。。。

The plasmid of pEGFP-N1 was preserved in our laboratory.②The rat vibrissa follicles were dissected under a stereomicroscope. The dermal sheath was moved by incubated in dispase. The bulge regions of the hair follicles in anagen phase were carefully cut between the arrector pili muscle and the sebaceous gland, and then incubated with a mixture of trypsin and EDTA. The cell suspension was selected, and cultured in 10% fetal bovine serum DMEM/F12 FAD medium. After 7 days culture, HFSC was obtained by rapid adhering on collagen Ⅳ.

实验方法：大鼠在体视显微镜下分离出含真皮鞘的完整毛囊，dispase消化，将毛囊从真皮鞘中挤出，收集形态完好且处于生长期的毛囊，分别在毛球部上端、皮脂腺下端横切毛囊，取中间部分置于胰酶和EDTA中联合消化，向所得细胞悬液中添加含10%胎牛血清DMEM/F12完全FAD培养基，常规培养7 d后，采用IV型胶原快速贴壁法两次筛选以分离纯化大鼠毛囊干细胞。

Basophilic. calcium deposits within the dermis that are variable in size. often surrounded by a foreign body type of granulomatous reaction.

嗜碱性。钙质沈积物存在于不同大小的真皮，这些真皮经常被俱肉芽瘤反应的外来体所包围。

Objective: To observe the attachment and growth of fibroblasts on a new porcine collagenic dermal matrix and investigate a method to improve the cell compatibility of the porcine dermal scaffold.

目的：观察成纤维细胞在一种新型的猪胶原基真皮支架上的粘附生长特性，探讨提高异种真皮支架细胞相容性的方法。

However, the molecular mechanisms underlying premature aging up 8-MOP/UVA are as yet unknown. In our studies, dermis of the non-lesion back skin of vulgar psoriatic patients who had been treated by PUVA and the cultured foetus dermic fibroblasts were selected to be observed.

本课题以PUVA治疗寻常型银屑病患者背部非皮损区真皮及培养的胎儿真皮成纤维细胞为对象，采用HE染色、Verhoeff染色、电镜、酶组织化学、免疫组织化学、原位杂交、流式细胞计数仪、三引物PCR、PCR-SSCP技术及PCR产物直接测序等技术和方法，研究了8-MOP/UVA对皮肤光老化形成的影响及芳维A酸乙酯的拮抗作用。

METHODS: Full-thickness skin was sterilely obtained from rabbit ears. 2 cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

无菌条件下切取兔耳全层皮肤，将2 cm×2 cm皮片置于0.25%胰酶-EDTA消化液中去掉表皮，再置入曲拉通X-100溶液使真皮细胞完全脱净，磷酸盐缓冲液反复清洗，即为脱细胞真皮基质，4 ℃保存备用。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。