真因子

OBJECTIVE: To clone mice CTGF gene and construct its eukaryotic expression vector, in addition, to investigate its transient expression in VSMC.

目的：克隆小鼠结缔组织生长因子基因、构建其真核表达载体，并在血管平滑肌细胞中瞬时表达。

The ubiquitin proteolytic system is an important selective proteolytic system of the celler proteolytic systems, a number of them were identified as a ubiquitin chain assembly factor-E4. The U-box protein is a new family ubiquitin-protein ligase(E3), which determine the substrate specificity of ubiquitination. The U-box is a domain of 70 amino acids that is highly conserved among yeast, plant and animals, but more U-box proteins were found in plants than in animals.

泛素系统是选择性降解细胞内蛋白质的重要系统之一，U-box蛋白质是此系统中决定底物特异性识别的一种新型E3蛋白质，部分U-box蛋白质属于泛素链聚集因子-E4.U-box结构域大约由70个氨基酸残基构成，在酵母、植物和动物等真核生物中保守存在，但植物中的数目远多于动物中。

OBJECTIVE: To construct a novel adenoviral eukaryotic expression vector that can co-express mutant hypoxia-inducible factor-1 alpha (HIF-1α) target protein and humanized Renilla reniformis green fluorescent protein reporter molecule under normoxic conditions.

目的：构建能够同时表达突变型低氧诱导因子1α(hypoxia inducible factor 1 alpha，HIF-1α)目的蛋白和人源化绿色荧光蛋白(human renilla reniformis green fluorescent protein，hrGFP)报告分子的新型腺病毒真核细胞表达载体。

Eukaryotic initiation factor 5A (eIF 5A) contains an unusual amino acid, hypusine, which is formed post translationally.

真核启始因子 5A(eIF 5A)翻译后第 4 9位的赖氨酸被修饰为一个特殊氨基酸hypusine ，后者是其发挥功能所必需的。

Transposable elements are important factors to cause genetic variation and recombination, which are widely spreaded in eukaryotic organisms.

转座元件是一类广泛分布于真核生物的可移动的遗传因子，可以引起基因重组和变异，在物种进化及遗传改良中起着重要作用。

DNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, which suggests that this modification must be tightly controlled.

DNA甲基化对于沉默的转座因子和真核细胞的某些基因是必不可少的，这表示DNA甲基化这一过程必须受到严格的控制。

Results The molecular relative mass of immunocytokine, IP10-scFv is 41.1 kD, affinity constant of IP10-scFv is 2.28×10^-8 with Scatchard formula.

结果重组免疫细胞因子（IP10-scFv）真核表达产物的相对分子质量约为41.1 10^3，纯化后的蛋白浓度为68mg／L，抗体亲和常数（KDIP10-scFv）为2.28 10^-8mol／L。

Methods CCL20 and HBsAg eukaryon expression vector was constructed by using DNA recombinant technology.

方法采用基因重组技术，构建趋化因子CCL20和表面抗原的真核表达载体，将重组载体用肌肉免疫的方式分别注射正常的C57BL/6小鼠，通过ELISA方法检测C57BL/6小鼠的抗-HBs抗体水平、细胞增殖情况。

Heat shock factor 1 (HSF1) is the master regulator of the heat shock response in eukaryotes, a very highly conserved protective mechanism.

热休克因子1（HSF-1）在真核生物中是主要的热休克反应调节物，热休克反应是一个高度保守的保护机制。

By a two-promoter vector, we successfully coexpressed DsbA-DsbC fusion protein with hNGF in procaryote cytoplasm and obtained the soluble hNGF protein, but the DsbA or DsbC alone do not show this function.

通过本课题的研究，我们根据DsbA和DsbA~蛋白的性质特点获得了一种原核通用融合表达载体，该载体在表达真核来源的活性蛋白或细胞因子具有促可溶和保持目的蛋白天然活性的功能。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。