真因子

ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

We make use of a normal element and circular permutations to construct polynomials over the intermediate extension field between F_q and F_ denoted by F_ where s divides t. The polynomials we constructed are F_q-linearly independent and return elements in F_q when they are evaluated at elements of the extension fields.

结合Reed-Solomon码的构造特点，我们将Chaoping Xing与San Ling所构造的线性码的方法推广到有限域的任意次扩张上，首先利用正规元和循环排列来构造系数属于F_q和F_q的任意次扩域F_之间的中间域的具有特殊性质的多项式，中间域记为F_，其中s为t的真因子，由正规元的选择可以保证所构造多项式的F_q-线性无关性，并且这些多项式在扩域F_中取值都属于F_q，从而构造出一类q元线性码。

Objective To clone human intestinal trefoil factor (hITF/hTFF3) gene into an eukaryotic expression vector for its expression in eukaryotic cells.

目的 构建人肠三叶因子(hITF/hTFF3)的真核载体并在真核细胞中表达。

Been used to express insulin, human growth factor, human tissue plasminogen activator, bovine chymosin (an enzyme used in cheese-making), and amylase and cellulase enzymes .

用作带有真核生物基因的载体的宿主用来表达胰鸟素、人类生长因子，人类组织血浆酶原活化因子，牛凝乳酶，淀粉酶、纤维素酶等。

Though the primary stimulator of 1H is not clear, it is related to physical, cell and body fluid IH is a chronic process involving multiple faction injury and blood flowing abnormal are revamped as the main reason, the proliferation level depend on the length and width of the injure of vessel large amount research illustrate injury cause many growth factor infolded in IH, for example: template derived growth factor, fibroblast growth factor, transmission growth factor , endothelin (ET-1) et al, some early responsive gene such as c-fos、c-jun c-myc also take part in SMC proliferation after vessel injury.

虽然IH的确切起始刺激因子并未阐明，但已知与物理、细胞和体液因子有关。IH是一个多因素参与的慢性过程，损伤和血液动力学异常一真被认为是IH的主要因素。内膜增殖的程度有赖于血管损伤的长度和深度。近年来大量的实验研究结果表明，损伤等刺激引起的众多的生长因子样物质在IH中起关键作用。如血小板源性生长因子、碱性纤维母细胞生长因子、转化生长因子β、内皮素-1（ET-1）等诸如C-fos、C-jun、C-myc等早期应答基因也参与了血管损伤后SMC的增殖。

It has been identified that its overacting is correlated to the ongoing and development of many diseases.

学术背景：核转录因子κB是普遍存在的真核细胞转录因子，已证明许多疾病的发生和发展都与它的过度活化有关。

The eukaryotic translation initiation factor 5A (eIF-5A) is a small acidic protein (18k Da), widely presents in all organisms from archebacteria to mammals but not in eubacteria, with highly conserved sequences. eIF5A is unique in that it is the only protein known to contain the unusual amino acid residue hypusine (hydroxyputrescine-lysine), which is essential for eIF5A function and is formed by post-translational modification of a specific lysine residue.

真核生物翻译起始因子5A(eIF5A)是一个分子量约18 kDa的酸性蛋白，其序列从古细菌到哺乳动物高度保守，并广泛存在于各种真核生物细胞中。eIF5A是迄今为止唯一一种已知含有特殊氨基酸残基hypusine的蛋白质。hypusine是一种蛋白翻译后修饰产物，它的形成对eIF5A的功能完整至关重要，相关的研究结果表明hypsine在控制细胞周期循环的过程当中扮演着一个重要的角色。

PART ONE CONSTRUCTION OF VECTOR CARRYING HUMAN INSULIN-LIKE GROWTH FACTOR-1 GENE AND THE GENE EXPRESSION IN EUCARYOTE Insulin-like growth factor-1(IGF-1) is one of the most important regulate factors which can stimulate cartilage cell to differentiate and synthesis collagen typeⅡand proteoglycan to maintain cartilage phaenotype.

第一部分：携带hIGF-1基因重组载体的构建及在真核细胞的表达胰岛素样生长因子-1(IGF-1)是在软骨发育和软骨自稳态调节中最重要的生长因子之一，能刺激软骨细胞分裂增殖，并促进软骨细胞合成Ⅱ型胶原和蛋白聚糖，维持软骨细胞的表型等。

In the medical field, using genetic engineering has successfully expressed many kinds of cytokines in protokaryon Bacillus coli, eukaryon yeast system and mammalian cells, which will make possibility of large-scale production of recombinant cytokines.

在医学领域，应用基因工程技术已在原核大肠杆菌、真核酵母和哺乳动物细胞成功表达了多种细胞因子，使重组细胞因子蛋白的大量生产和应用成为可能。

Heat shock factor 1 (HSF1) is the major regulator of the heat shock response in eukaryotes.

热休克因子1 (heat shock factor 1，HSF1)是调控真核生物热休克反应的主要转录因子。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。