真

Filicinophyta The largest phylum of spore-bearing vascular plants comprising the ferns and including about 12000 species. It is divided into two main groups, the LEPTOSPORANGIATE ferns and the EUSPORANGIATE ferns.

真蕨门：是有孢子维管植物中最大的一个门，包含真蕨类，约12，000余种，主要分为薄囊蕨和厚囊蕨两种。

Of or belonging to the infraclass Eutheria, a division of mammals to which all the placental mammals belong.

真哺乳亚纲的关于或属于哺乳动物真兽亚纲的一个下纲，包括有胎盘哺乳动物

Of or belong ing to the infraclass Eutheria,a division of mammals to which all the placental mammals belong .

真哺乳亚纲的关于或属于哺乳动物真兽亚纲的一个下纲，包括有胎盘哺乳动物

These days I often fell exite about this,I really want to possess the romantic love like yours ,I really want to possess the marriage like yours!

（这些天我常常因此而激动不已，我真想拥有像你们一样的爱情，我真想拥有像你们一样的婚姻！

The structure of spore wall of more than 10 species of the fern Filicales and the structure and development of sporoderm of 7 representative species of Filicales were studied contrastively by Light microscope, scanning electron microscope and transmission electron microscope.

主要采用光镜、扫描电镜和透射电镜对照的方法，对我国产真蕨目10多种真蕨孢子主要类型的壁层结构进行了观察，并对7种典型类型的代表种进行了孢壁结构和发育的研究。

Subtle friendship is true;subtle greetings are enough;subtle love is tender;subtle longing is deep;subtle wisnes come frim the bottom of your heart.

淡淡的一点友谊很真，淡淡的一点问候很醇，淡淡的一点依恋很清，淡淡的一点孤独很美，淡淡的一点思念很深，淡淡的一点祝福最真。

As one of Deutsche GEMMY Art grounds brand authorized suppliers in mainland China and the Performer of various support services, king Qi not only try to promote and market the world's most well-known brand "GEMMY", but also develop new kinds of products in cooperation with the Chinese Academy of Science using the patented technology ,such as the combination of nano-technology and materials to develop a permeable ground.

作为德国真石丽艺术地坪地坪品牌中国大陆授权供货商以及各项支持服务的执行者，在推广和营销全球最知名品牌&真石丽&同时，上海景琪还联合全国科研院所开发自主知识产权的新技术、新产品，包括制品加工新技术等，如将纳米技术与建筑材料结合，开发出透水地坪。

"Unreal is real and the real is also false, there is inaction there is also no," Jia Jia Zhen, one blurred,"A Dream of Red Mansions" of all, Lin Tai-yu as a soul, and her people, she is her A cluster of lofty Guao, Gufangzishang Valley of the Orchids.

&假作真时真亦假，无为有处有还无&，假假真真，让人琢磨不透《红楼梦》中的一切，林黛玉作为灵魂人物，她与常人不同，她就是她，一丛清高孤傲的、孤芳自赏的空谷幽兰。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

Arabidopsis response regulators,ARR4 and ARR5, were induced by the increased cytokinin contents at different time after seed germination. ARR4 responded to cytokinins at early time of seed germination, especially on the 6 d when seedlings were around true leaf initiation, while cytokinins induced ARR5 activation after 6 d of seed cultivation in light conditions, an obvious increase was on the 20 d when seedlings were around inflorescent shoot initiation.

拟南芥植物细胞分裂素反应调节基因ARR4和ARR5在植物发育的不同时期应答植物体内增加的植物细胞分裂素，ARR4的应答反应比ARR5早，种子萌发后的第6天幼苗真叶形成初期，ARR4基因被明显诱导；而ARR5的应答反应在幼苗真叶形成后的几个时间段均能检测到，并且在种子萌发后的第20天，花枝形成开始时特别明显。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。