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Bone marrow was drawn from rabbit by density gradient centrifugate in 70% Percoll fluid, the rate of cell survival was evaluated with trypan blue staining;The cells have been induced,differentiated and transfered of culture in vitro for five weeks; MSCs was seed onto BMG and continued to be cultured six weeks in vitro,then the compound was carried out to observe collagen by Masson staining.

抽取兔骨髓,用70% Percoll液,经密度梯度离心法分离MSCs,台蓝染色观察细胞存活率;诱导分化,传代,体外培养5周;复合BMG,继续体外培养6周,Masson染色观察胶原合成情况。

As the essential component of Chinese culture, traditional favonian pattern is a kind of formalism in the art field.

我们把这些具有历史渊源,富于民间特色,又蕴含吉祥祈的图案,称之为中国传统吉祥图案。

My parents waited formy cryusing ten months, whileI wait for beingquiteusing my whole life.

爸妈用十个月我啼哭,而我用一辈子望沉寂。

Gerontal Gastroenterology Department, General Hospital of PLA, Beijing 100853, China

作者:赵擎,辛绍杰,侯俊,胡燕,貌勇作者单位:北京中国人民解放军总医院南楼消化科,100853;北京解放军第三〇二医院传染三科,100039北京解放军第三〇二医院传染病研究所病毒研究室,00039

Gerontal Gastroenterology Department, General Hospital of PLA, Beijing 100853, China

赵擎,北京中国人民解放军总医院南楼消化科,100853;辛绍杰,北京解放军第三〇二医院传染三科,100039;侯俊、胡燕、貌勇,北京解放军第三〇二医院传染病研究所病毒研究室,00039

The first part is Introduction: research overview for "feminine consciousness" of The Book of Songs; Chapter 1: See "feminine consciousness" of The Book of Songs from the content of the text. Chapter 1 is divided into five minor points of conducts. Section 1: The life makes open hypostatically- free equal consciousness; Section 2: The desire of the soul integrity - love consciousness; Section 3: The realistic expectation of the marriage mode- the consciousness of hoping to get married; Section 4: The morals of the ethical relations marriage pursue- the good wife consciousness; Section 5: The instinct of the life value assurance- the consciousness of growing. Chapter 2: See "feminine consciousness" of The Book of Songs from the art appearance characteristic. Chapter 2 is divided into two minor points of conducts, section 1: With the females eye view thing: Female taste of the idea choice; Section 2: With the female's a feeling of heart: Female thinking mode of the woman keeps in well mind ". Chapter 3: The social source of the "feminine

论文主要分为四部分,即绪论:《诗经》女性意识研究综述;第一章:从文本内容看《诗经》的女性意识,分为五小节,第一节:生命本质的张扬——自由平等意识;第二节:性灵完整的渴望——爱情意识;第三节:婚姻模式的现实期待——嫁意识;第四节:人伦婚姻的道德追求——贤妻意识;第五节:人生价值的本能确定——生育意识;第二章:从艺术形态特征看《诗经》的女性意识,分为两小节,第一节:意象选择的女性眼光;第二节:&女子善怀&的思维模式;第三章:《诗经》女性意识产生的社会根源,分为四小节,第一节:原始群婚风俗的残余;第二节:礼制初成时期女性的相对自由;第三节:尊男卑女的社会文化制度;第四节:战争徭役带来的男女失衡。

Still be an unknown at present, but it appears on the market inferiorly doorsill and wealth effect can give a person with infinite imaginary space, raise one's head and look making a person in order to long for.

现在还是一个未知数,但它较低的上市门槛和财富效应能给人以无限的想象空间,令人翘首以

The experimental group corneas were preserved by organ culture for 4 weeks, the corneal thickness was measured with ultrasonic corneal pachymeter. Then every corneas were divided into half -chip, there are 48 half-chip total. It was divided into 4 groups, there are 12 half-chip in every groups. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution, HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

实验组经器官培养保存4周后以角膜测厚仪测量角膜厚度,然后每个角膜被分成两半,共48个半片角膜,再分成4组,每组12个半片。12个半片用茜素红-台蓝染色染色行角膜内皮细胞计数;12个半片角膜用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;12个半片角膜用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;12个半片角膜用实时荧光定量PCR检测AQP-1mRNA表达改变。

The general situations of the eye were observed and the corneal thickness were measured with ultrasonic corneal pachymeter after the animal models was established. After a week, the corneas were removed after the experimental animals are put to death. The corneal endothelial cell density of 12 half-chip were counted through Alizarin Red-Trypan blue staining; 12 half-clip corneas were fixed with 4% neutral formalin solution , HE staining was performed, the expression of AQP-1 in corneal stroma and corneal endothelial cell were detected through immunohistochemical staining; Na~+-K~+-ATPase activities in 12 half-clip corneas were examined with Na~+-K~+-ATPase kit; the expression of AQP-1 mRNA were detected through real-time fluorescent quantitation PCR.

术后观察眼球大体情况、测量角膜厚度。1周后处死实验动物取角膜,用茜素红-台蓝染色染色行角膜内皮细胞计数;用4%中性福尔马林溶液固定行HE染色、应用免疫组化染色检测AQP-1在角膜基质和内皮细胞表达的改变;用Na~+-K~+-ATP酶试剂盒测量角膜内皮细胞Na~+-K~+-ATP酶活性;实时荧光定量PCR检测AQP-1mRNA在角膜内皮细胞表达的改变;并于正常对照组角膜比较。

The birds of Passeriformes are the group which are the largest number, the most species, the highest station of evolvement and the broadest adaptive radiation of existent aves. The traditional taxonomy were onlyaccording to morphologic and behavioral characters, however their morphologic and behavioral characters are so complicated that we could not differentiate some species easily. Therefore we had some taxonomic research by molecular means in order to supply traditional taxonomy.

雀形目是现存鸟类中种类最多、数量最大的、演化地位最高、适应辐射最广的类群,传统的分类方法仅依据形态和行为特征来划分,但由于该类群的形态及行为特征复杂使得部分种类不易区分,因此有必要依据分子手段研究其系统发生关系、以能对经典的分类方法给予补充。

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推荐网络例句

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候,里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色,卵在发育96h后卵基本全部孵化。

There was a conflict between plebs and patricians in ancient Rome in 494BC.

在公元前494年,罗马发生了一次平民反对贵族的斗争。