相术

Results:Comparing to the control group, the proliferation of overexpressed p16 QBC939 cells was inhibited and p16 overexpress induced G1 phase blockade.

结果：与对照组相比，p16高表达的人胆管癌细胞QBC939的增殖受到明显抑制；流式细胞光度术表明p16的高表达导致了较为明显的G1期阻滞作用，但对细胞周期的其他时相并无影响；Western免疫印迹分析结果表明癌基因c-myc的蛋白水平表达下降。

Objective To study the statement of immunoreaction of c Jun and functional recovery of spinal cord injury in rats after fetal spinal cord transplantation.Methods Experimental rats were divided into two groups:spinal cord hemisection injury combination with transplants of FSC to injured site group,and spinal cord injury that received gelfoam pledgets group.On the lst,3th,7th,14th and 28th day after operation,6 animals in each group at each time phase,were observed for their function recovery with the methods of praxiology and electro physiological examination,the animals were then sacrificed for the observation of the statement of c Jun reaction by immnochemistry method and computer image analysis system was used for quantitative analysis.

目的：研究大鼠胚胎脊髓移植后能否影响，c-Hun免疫反应的表达和脊髓损伤后大鼠功能的恢复，方法；将动物分为脊髓半切洞损伤加胚胎脊髓移植组和单纯脊髓板切洞损伤加明胶海绵填塞组，每个时相点每组6只动物，术只1，3，7，14和28天，应用行为学和电生理检查观察大鼠功能恢复情况，应用免疫细胞化学方法，c-Jun免疫反应的表达，采用计算机图像分析技术，进行定量分析。

All other preoperative parameters were similar in both groups.

两组间其他所有的术前参数是相似的。

①Rat MSC and VSMC were cultured and identified, respectively. MSC were labeled with DAPI firstly, and then co-cultivated with VSMC. The changes of morphology and ultrastructure of co-cultured cells were observed. Immunfluorescence analysis was performed by using monoclonal antibodies against specific antigen.②We established the regulatable system in two steps: a stable MSC line expressing rtTA has been constructed and characterized firstly by transfected with pUHD 17-1hyg and then selected by hygromycin B; in a second step, this line was used for trandfer the AT2R gene to MSC to get the well establishing double stable MSC lines;③The expression of AT2R regulated by doxycycline was evaluated by western blot;④The MSCs were transduced into rat carotid arteries with regulatable AT2R gene after the establishment of rat carotid balloon injury restenosis model. The intimal/medial area ratio were measured by digital analysis system.

研究方法：(1)密度梯度离心法及胶原酶消化法分别培养原代大鼠MSC及VSMC，细胞共培养并行免疫荧光化学染色和透射电镜观察超微结构；(2)组成受Dox调控的哺乳动物表达系统的四种成分的转化、扩增及提纯并酶切鉴定；(3)采用常规分子生物学方法连续两个回合转染体外培养的MSC，并分别采用发光计检测不同细胞克隆萤光素酶活性改变以及RT-PCR方法检测AT2R目的基因mRNA表达情况，根据各个细胞克隆受Dox调控表达的程度，选择低背景、高诱导表达AT2R的细胞系，作为双重稳定MSC细胞系；蛋白免疫印迹法观察该细胞系在Dox调控下AT2R表达的时相性、持续性及在不同浓度Dox调控下的表达情况；(4)建立大鼠颈动脉球囊损伤动物模型，将双重稳定MSC在术中导入血管，分别于14 d、28 d进行病理切片，检测可调控AT2R对新生内膜增生的影响；采用RT-PCR免疫组织化学免疫荧光等技术观察AT2R基因在新生内膜中的表达以及细胞外基质成分表达的改变，TUNEL法检测血管组织中细胞凋亡的变化情况。

Spinal fusion (officially known as spondylosyndesis) is a surgery that combines two or more vertebrae, fusing them together.

脊柱融合术（正式名称为spondylosyndesis ）是一种外科手术相结合的两个或两个以上的椎骨，融合一起。

Then, a subsequential study was carried out as an application of DNA origami: molecular electronic circuit constructed by DNA origami combining with DNA metallization.

接着，本文就DNA折纸术的一个发展方向进行了更深入的研究：和DNA金属化相结合构造分子电子器件。

Abstract］ Objective To evaluate the distinctive clinical value of magnetic resonance urographyMRU）in the diagnosis of primary ureteral carcinoma.Methods To compare the accuracy of diagnosis of MRU with ultrasonography,urography,retrograde ureteropyelography and CT,diagnostic imaging and progress of ureteral carcinoma were analyzed in twenty-four cases from 2000 to 2006,twenty cases had MRU examinations.The original preoperative diagnostic reports of various imaging facilities were analyzed on account of the results of operation and pathology.

目的 探讨磁共振水成像技术在原发性输尿管肿瘤中的临床诊断价值方法分析2000～2006年24例原发性输尿管肿瘤的影像学诊断结果，并基于术中所见和病理报告，将磁共振水成像技术20例）与超声24例）、泌尿系造影24例）、逆行造影15例）、CT(19例）的确诊率相比较。

Methods To compare the accuracy of diagnosis of MRU with ultrasonography,urographyKUB+IVP）,retrograde ureteropyelography and CT,diagnostic imaging and progress of ureteral carcinoma were analyzed in twenty-four cases from 2000 to 2006,twenty cases had MRU examinations.The original preoperative diagnostic reports of various imaging facilities were analyzed on account of the results of operation and pathology.

分析2000～2006年24例原发性输尿管肿瘤的影像学诊断结果，并基于术中所见和病理报告，将磁共振水成像技术20例）与超声24例）、泌尿系造影24例）、逆行造影15例）、CT(19例）的确诊率相比较。

Pass to the hospital about the treatment of this disease is to wash lacrimal duct and lacrimal passage combine.

到医院去通一下，这种病的治疗方法是泪道冲洗法和泪道探通术相结合。

Against the $6000 Musical Fidelity Tri-Vista SACD player—using only CDs, of course—and again with levels matched at 1kHz, the No.390S's soundstage was actually a little deeper than that of the tubed player, but its presence region was a little more forward, its midrange very slightly less rich-sounding.

相对于 $6000 音乐的忠诚三街景的 SACD 使用运动员的只有激光唱碟，当然-而且再一次以以 1 仟赫被相配的水平， No.390S's 的 soundstage 是比被装入桶的运动员实际上稍微深，但是它的出现区域稍微更向前，非常些微比较不富有的它中间范围-探通术。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。