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Making gfp fusion protein is a convenient method to mark the expression of interesting proteins, but it may interfere the expression of interesting proteins, since gfp is a quite big protein itself.

一方面,作为标记与目的蛋白融合可以方便的标示目的蛋白的表达情况,但gfp本身是个相当大的蛋白,会降低目的蛋白的表达;另一方面,进一步分离时需使用抗gfp的单克隆抗体进行免疫共沉淀,既不经济又不方便。

This takes considerable dramatic licence with history, emphasising the importance of Farinelli's brother and reducing Porpora's role, while Handel becomes an antagonist; the singer's time in Spain is ignored almost entirely.

这部片子藉历史作相当大的戏剧渲染,剧中强调他哥哥的重要性而降低Porpora的地位,而韩德尔变成他的对立者,他待在西班牙的时光几乎全被忽略掉。

For the sake of matching foam fluid frilling technology, international developed country has developed a series of foam fluid process technology, which run through each process of drilling, consummate well, increase output, exploitive and dynamic adjust.

为了与泡沫流体钻井技术相配套,国际上发达国家发展了一系列的泡沫流体作业技术,贯穿于钻井、完井、增产改造、开发及动态调整的各个过程。预计泡沫技术在我国会有相当大的应用前景。而在所有泡沫流体的作业中,一项共同的、重要的基础工作是研究、测定高温高压条件下的泡沫流体特性。

But you need to understand that there are gigantic opportunities in China and gigantic changes taking place there.

然而,你必须明白的是,中国发生了巨大的变化,同时也存在这相当大的机遇,所以我的书旨在以简单的叙述告诉人们,中国发生了什么?

Grid, or the size of price gap between rental units, from two angles calculated rate of return on investment should be relatively high, so, the long-term demand There was still a considerable amount of market share.

电网,或大小的价格出租单位之间的差距,从两个角度计算的投资回报率应该比较高,因此,从长远长期的需求仍然存在着相当大的市场份额。

This phenomenon was mainly attributable, on the one hand, in recent years, multinational companies entering China is not a substantial increase; the other hand, some large multinational companies started to take employee localization strategy, a substantial reduction in staff housing on the hire-purchase budget, which led to a corresponding decrease in demand; In addition, new projects in the rapidly improve the cost-effective, every introduction of a new disk of the old site have a considerable impact.

这种现象的主要原因是,一方面,近年来,跨国公司进入中国是不是一个大幅度增加;另一方面,一些大型跨国公司开始采取员工本土化战略,大幅度减少工作人员住房的租购买预算,从而导致相应减少需求;此外,新项目的迅速提高成本效益,每引进一个新的磁盘的旧网站产生相当大的影响。

Inertial force of crankshaft-connecting rod in a V-cylinder engine have influence on vibration and comfort in vehicle.

V型双缸发动机中曲柄连杆机构的惯性力的大小与方向对车辆的振动和舒适性方面有相当大的影响,在摩托车发动机中一般采用过量平衡调整一次惯性力的方法,但长期以来缺乏有效的实验研究手段。

The great jurisconsult himself scouts the popular apology offered for it in the mental inferiority of the female sex, and a considerable part of his volume is taken up with descriptions of the numerous expedients, some of them displaying extraordinary ingenuity, which the Roman lawyers had devised for enabling Women to defeat the ancient rules.

这个伟大的法学专家斥责了一般用来替这个制度辩解的所谓女性智力低劣的说法,在他的著作中,并且有相当大的一部分用来说明罗马法学家所提出以便"妇女们"能凭以打垮这古代规定的许多方法,其中有些是非常聪明的。

Shifting migratory routes for endangered species such as the leatherback turtle could complicate what are already tricky conservation challenges for wandering sea creatures, says Lee Hannah of Conservation International in Washington, D.C.:"They re going to start turning up in places where there may not be conservation measures in place."

濒危物种迁徙路线的改变使已有的保护措施更加复杂化例如革龟,这些保护措施本身对于四处游走的海洋生物已是一个相当大的挑战,位于华盛顿的国际环保组织的李汉娜说,"这些物种开始在没有采取保护措施的海域出没。"

It should be in this industry inside contribute somewhat, in the technology development respect wants to be contributed somewhat, should assume case " great " these two words, it has nature quite large scale, continuously profit grows, give partner good get one's own back, give employee very good platform and very good material drive, want not let sb down whole society, this ability deserve to be called a great company.

它应该在这个行业里边有所贡献,在技术发展方面要有所贡献,要承担得起"伟大"这两个字,自然它有相当大的规模,持续地利润增长,给股东好的回报,给员工很好的平台和很好的物质激励,要对得起整个社会,这才称得起一家伟大的公司。

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Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势,并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。

This dramatic range, steeper than the Himalayas, is the upturned rim of the eastern edge of Tibet, a plateau that has risen to 5 km in response to the slow but un stoppable collision of India with Asia that began about 55 million years ago and which continues unabated today.

这一引人注目的地域范围,比喜马拉雅山更加陡峭,是处于西藏东部边缘的朝上翻的边框地带。响应启始于约5500万年前的、缓慢的但却不可阻挡的印度与亚洲地壳板块碰撞,高原已上升至五千米,这种碰撞持续至今,毫无衰退。