相变性

The invasion of drilling fluid is the main cause to the damage of fractured formation. The composite salt free-gel drilling fluid is got after analyzing the influence of different kinds of such soluble adulterant as organic salt and non organic salt on the performance of the solid-free drilling fluid and studying the condition to develop the free gel structure and the method to improve the fluid's high temperature stability and inhibitive property. The experiment shows that the apparent viscosity of this system is as high as 27000 mPas when the share rate is 0.3 r/min and the high temperature reaches at the stable level of 150℃ and the HTHP filtrate loss decreases to 15 ml at 150℃.

钻井液侵入是裂缝性储层损害的主要因素之一，实验分析了无机盐、有机盐等不同类型水溶性加重剂对无固相钻井液性能的影响，研究了无固相钻井液快速弱凝胶特性形成条件以及高温稳定性和抑制能力的提高方法，研制出的无固相复合盐弱凝胶功能性钻井液高温稳定性达到150℃，具有独特的流变性（0.3r/min黏度达到27000 mPas）和优良的抑制能力（150℃HTHP滤失量小于15 mL），成本远低于甲酸盐钻井完井液。

Firstly,The crosslink of sodium caseinate and whey protein isolateat three different conditions using transglutaminasewas studied.The extent of polymerization was detemined by SDS-PAGE、electron microscope and infrared spectrometer associated with gel image analysis.

通过对各组分蛋白相对光密度值的变化分析可知：在本实验所选条件下，在80℃预热15min后并再加入一定量还原剂(20mmoL/LDTT)变性条件下，酪蛋白和乳清蛋白之间可以交联；为进一步验证上述结论，通过中红外光谱仪和电子显微镜进一步分析交联前后蛋白质的二级结构的变化和交联前后蛋白质空间结构上的差异，结果分析均与上述结论相吻合。

The SiC slurry catched high thixotropy when the SiC powders were coated all by hydrophile groups, and the viscosity of the SiC slurry would increase 1236.0mPas suddenly when its solid loading added from 57.4% to 58.5%; If the SiC powders were coated by lipophile group, the thixotropy of the SiC slurry would reduce and the enhancive scope of the high solid loading SiC slurry's viscosity would also be weakened.

改性粉表面全是亲水基团时，料浆触变性大，当固相体积含量由57.4%上升到58.5%时，料浆粘度突然增大1236.0mPaS；随着亲油基团的引入，料浆触变性降低，高固相含量时粘度增大平缓。

On the basis of the kinetic equation of substrate reaction in the presence of urea or guanidine hydrochloride, all microscopic kinetic constants for the free enzyme and enzyme-substrate binary and ternary complexes have been determined. The results of the present studies indicate that:①In the presence of urea or guanidine hydrochloride, enzyme-substrate complexes lose their activity less rapidly than the free enzyme. Therefore, both substrates, NADPH and 7, 8-dihydrofolate, protect dihydrofolate reductase against inactivation.②The denaturation of dihydrofolate reductase by urea follows single-phase kinetics, and changes in enzyme activity and tertiary structure proceed simultaneously in the unfolding process, so it may be an"all or none"process.③The GdnHCl-induced unfolding of the dihydrofolate shows a biphasic transition, while the change in the enzyme activity is a single exponential process. The rate constant of inactivation is consistent with that of the fast conformational change. Therefore, the kinetic intermediate of protein unfolding should be a partially folded and inactive form.

我们根据在脲或盐酸胍存在下的底物反应动力学方程求得游离酶和酶底物二元、三元复合物的微观动力学常数，结果表明：①酶-底物二元、三元复合物的失活速度明显慢于游离酶，说明两个底物二氢叶酸和NADPH对酶的失活都具有一定程度的保护作用；②在脲作用下，酶的失活和构象变化均为单指数项过程，而且酶的活力丧失和三级结构变化是同时发生的，说明二氢叶酸还原酶的脲变性可能是一个"全或无"的两态过程；③在盐酸胍作用下，酶的构象变化为两相过程，而失活则是单指数项过程，酶分子构象变化的快相速度常数与失活速度常数基本一致，因此我们认为二氢叶酸还原酶的盐酸胍变性过程中存在一个没有活力、但仍具备一定空间结构的变性中间体。

The resolution and renaturation efficiency of lysozyme were found to be independent of the flow rate and gradient time when the volume of mobile phase was kept a constant.

在计量置换模型的基础上首次提出了流动相用量估算公式，用实验对其进行了检验，并得出在保持流动相用量不变的条件下，改变流速和梯度时间对分离度和变性蛋白的复性效率影响不大的结论。

The effects of BN content on the thermal conductivity, hardness and microstructure of AlN-BN composite ceramics prepared by ceramic injection molding and pressureless sintering, as well as the rheological characteristics of the mixtures were discussed.

采用粉末注射成形和无压烧结相结合的工艺制备AlN-BN复相陶瓷，讨论了AlN-BN混合料的流变性能以及BN含量对复相陶瓷热导率、硬度以及显微组织的影响。

The present work studied the morphology and properties of ternary blends composed of thermoplastic, glass fiber and thermotropic liquid crystalline polymer.

本论文研究了由热塑性树脂、宏观纤维和热致液晶聚合物组成的多相共混体系中，组分形态和相界面作用等因素与体系加工流变性能和其他性能的内在联系，以及这些因素对液晶聚合物和宏观纤维几何尺寸与分布的影响规律，从流变学、形态学和力学上认识形状与尺寸不同的两种分散相对体系产生的影响。

Skill-mental dominated event-group has characteristics what are polarizing frighten goal and fluctuating variation course. 6 Coaches and athletes must insist on the principles that is comminuting of judging accurately and intervening in good time, perspective with getting practical to combine together, timeliness and synchronous combine together, controllability and principle that substantial results combine together.

影响竞赛过程中运动员竞技能力变异的众多因素可聚合为以下四个相对独立又相互联系的子系统，即：运动员的竞技状态与相对竞技实力、参赛认知水平与比赛经验、可逆转环境和中立环境。4运动员竞技能力的参赛变异具有变异成因的多元性和多变性、变异内容体能的先导性、变异过程的可控性与非可控性、变异结果的两极性和层次性和参赛行为的心态易变性等五个方面的一般训练学特征。5体能主导类项群具有变异结果直观性、变异过程连续性和对手制约两重性等特征；技能主导类项群具有单项贡献制约性、个体变异传递性和裁判评定诱导性等特征；技战能主导类项群具有群体整合共生性、核心技战术制导性、攻防转换制约性和局盘赛点突增性等特征；技心能主导类项群具有目标恐惧极化性和变异过程波动性等特征。6教练员、运动员必须坚持准确判断和适时干预相结合、前瞻性与务实性相结合、适时性与同步性相结合、可控性与实效性相结合的原则。

Albicans strains with fluconazole. Method: A series of 16 strains C. albicans from the same body were induced to form pseudo-hyphae in RPMI 1640 medium and DMEM medium at 37 癈 for twenty-four hours respectively. The pseudo-hyphae and yeast were counted and the ratio between pseudo-hypha and total cells was calculated. C. albicans strains were induced to form pseudo-hyphae in RPMI 1640 medium for seven days by transferring twelve times. The genome DNAs of the hyphal-form of C. albicans was cleaved by two restriction enzymes EcoR I and Bgl II, the products were electrophored and photoed.

以同一母体来源的白念珠菌CA-1～A-17(共16株)作为研究对象，采用RPMI1640和DMEM培养基，37℃培养24h诱导其假菌丝形成，记录不同观察时间时的菌丝相和酵母相细胞数，计算菌丝相白念珠菌形成率；以RPMI1640培养基，37℃连续传代培养7天(转种12次)后，分离菌丝相白念珠菌并提取其基因组DNA；以EcoRⅠ和BglⅡ消化菌丝相基因组DNA，消化产物进行琼脂糖凝胶电泳并拍照；用随机引物(5'…TCACCACGGT…3')扩增菌丝相基因组DNA，采用变性聚丙烯酰胺凝胶电泳分离扩增产物并拍照。

The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明，当变性液中盐酸胍浓度约为1.0 mol/L时，芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体，其去折叠过程符合&三态模型&；荧光探针结果表明，在溶液中盐酸胍浓度约为1.0 mol/L时，中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域；荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况，结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个；蛋白电泳和体积排阻色谱结果表明，在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中，不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。