相关物

Abnormal expressions of Ki67 and PTEN protein were associated with can ceration in stomach. Therefore, both of them may act as useful biomarkers for early detection of gastric carcinoma.

Ki67和PTEN蛋白的表达异常与胃癌的癌变过程相关，两者有可能成为胃组织早期癌变的分子标记物。

Four experiments were primarily studied the antimicrobial ability and activity of the antimicrobial peptides crude sample from young Chelydra serpentina's intestines,blood,liver,skin,moreover were studied the blood antimicrobial peptides' purification process and their related characteristic.

本文初步研究鳄龟肠道、血液、肝脏和皮肤组织粗提物的抗菌活性，在此基础上进一步研究了鳄龟血液抗菌肽的分离纯化过程及其相关性质。

Characterization of the tomato Cf4 gene for resistance to Cladosporium fulvum identifies sequences that determine recognitional specificity in Cf4 and Cf9 .

利用抗病基因保守区域设计引物，进行植物基因组DNA扩增，以得到与抗病基因相关的分子标记，许多学者都有过成功的报道。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Firstly, the recombinant engineering technology related vaccine preparation was set up, including: 1. the gene knock-out based on linear DNA target molecular in vivo; 2. the selection and counterselection technology of exogenous gene knock in definite site on chromosome; 3. the Gap-repair clone technology in vivo; 4. the recombination technology of single-chain DNA and the overlapping primer; 5. Set up the new selection and counterselection technology of neo-E.

本研究首先建立与疫苗制备相关的重组工程技术包括：①基于线型DNA打靶分子的大肠杆菌体内基因剔除技术，②选择与反选择外源基因定位敲入染色体上特定位点技术，③Gap-repair体内克隆技术，④单链DNA重组技术和重叠引物介导的DNA重组技术，⑤建立&抗生素-酶切位点&选择反选择技术新技术。

Methods compare with sysmex-r3500 analyzer、coulter maxm analyzer、miller ocularto determine 130 ordinary samples.results f=3.87,p.05,the difference is conspicuous.each"r"is 0.7331,0.7231,0.5011,each other are beeline correlation.about sysmex-r3500 analyzer,the inter-assay cv of quality control cv=2.95%,the sample is cv=4.42%;about couter maxm analyzer,the intra-assay cv is 5.70%,intra-assay cv is 17.02%;about miller ocular,the inter-assay cv is 26.15%.conclusion sysmex-r3500 analyzer is good at repeatability,convenience,quickness,so it is adapt to clinical batch samples quickly determine.

分别用sysmex-r3500分析仪，coulter maxm分析仪以及国际血液标准化委员会所推荐的miller窥盘法进行比较，对130份正常人标本进行网织红细胞测定。结果：f=3.87，p.05，差异有显著性。其两两之间r值分别是0.7331，0.7231，0.5011，两两之间直线相关。sysmex-r3500分析仪的质控物批内平均cv=2.95%，标本批内平均cv=4.42%，批间平均cv=3.42%；coulter maxm分析仪批内平均cv=5.70%，批间平均cv=17.02%；miller窥盘法批内平均cv=26.15%。结论：sysmex-r3500分析仪具有重复性好，简便，快捷的特点，尤其适合临床大批标本的快速测定。网织红细胞 miller；窥盘法；仪器法

Trace element record is formed by the coupling action of early diagenetic perturbation and human pollution, and usually showes the covariance with iron or manganese record.

沉积物微量元素记录是早期成岩改造和人为活动污染综合作用的结果，往往与Fe或Mn的记录呈相关波动。

Because of the relation of positive sequence of the production and deposition time of dissolvable TR compound, in certain years, the "red" of Pu'er Tea is an important index to identify the aged period of Pu'er Tea.

由于不溶性茶红素复合物的产生与存放时间的正相关关系，在一定的年限内，普洱茶的&红&是鉴别普洱茶陈期的重要指标。

So doughtily and drily did he stick to his tale (and kept a camera with him ever after, to take the aliens' pictures if they ever came for him again) that the police opened a criminal investigation for assault, the only one in Britain to arise from a UFO "sighting".

他固执而冷漠地坚持自己的说法（并在那之后随身携带摄像机，以备外星人再来找他时可以拍下照片），警察只好展开相关犯罪调查。这是英国唯一一起有关&看见不明飞行物&的案子。

Nm23, VEGF and ER can be used as markers in the prognostication of breast cancer. Patients with nm23, ER positive and VEGF negative have better postoperative prognosis. Expression of ER can be used as an early marker, Postmenopausal breast cancer patient should apply more active endocrinotherapy.

我们认为，乳腺癌的发生与癌组织中的 nln23、pZI VEGF和 ER过度表达有密切相关，nln23阳性、VEGF阴性和 ER 阳性的乳腺癌术后预后较好，癌组织中的m23、VE GF和ER表达可以作为判断乳腺癌预后的指标；ER的表达可以作为乳腺癌的早期标记物；绝经后乳腺癌病人应采取更积极的内分泌治疗。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。