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The main virus that infected oilseed rape and the variance of its related isolates were confirmed.

明确了油菜病毒病的主要毒源及相关分离物的分类归属。

Of, relating to, or belonging to the geologic time, system of rocks, and sedimentary deposits of the first period of the Paleozoic Era, characterized by warm seas and desert land areas.

寒武纪的寒武纪的、与之相关的或属于它的,即古新世时代第一时期的岩石体系的以及沉积物的,以海洋的温暖和陆地的荒漠为特征

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能,本研究选择了MAP的主要保护性抗原Hsp65蛋白,以副结核分枝杆菌C-2株染色体DNA为模板,以hsp65基因的特异性引物进行PCR扩增,获得了1 626bp的hsp65基因,通过T-A克隆技术,将PCR产物克隆至pGEM-T Vector中,以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆,成功地构建出克隆质粒pGEM-T-hsp65,以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列,结果表明,所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致,两者核苷酸序列的同源性为99.1%,氨基酸序列的同源性为99.3%,表明该基因在副结核分枝杆菌中高度保守。

Huanghe River Estuary ; sediment ; geochemical form ; partial correlation coefficient

黄河口;沉积物;地球化学形式;偏相关系数

In the best condition of headspace and chromatogram, the mensuration of aquatic trace volatile can get a satisfaction result such as chloroform , carbon tetrachloride , trichloroethylene and perchlorethylene, all relativity standard deviation RSD is less than 4%,all the correlation coefficient R is larger than 0.99, and the relative error is less than 3%.

在选择最佳的顶空和色谱条件下,测定水中痕量挥发物三氯甲烷,四氯化碳,三氯乙烯和四氯乙烯可获得令人满意的结果,相对标准偏差RSD均小于4%,相关系数R均大于0.99,相对误差小于3%。

However, enhanced phosphorescence shows no clear correlation with the stability constants of ternary complexes and the type of the surfactants.

但三元包络物的表观稳定常数和表面活性剂类型与体系磷光强弱不呈相关关系。

Ournal of Applied Phycology publishes original articles relevant to practical uses of algae, including fundamental research, development of techniques and commercial applications. The scope includes algal biotechnology and genetic engineering, tissue culture, culture collections, commercially useful micro-algae and their products, marine culture, algal inoculation and soil fertility, pollution and fouling, monitoring, toxicity tests, toxic compounds and antibiotics.

应用藻类学杂志》发表与藻类实际应用相关的原始文献,包括藻类学基础研究,技术开发和商业应用,范围涉及藻类生物技术,遗传工程,组织培养,培养物收集,适用于商业性利用的微藻及其产物,海水培养,藻类接种和土壤肥力、污染和发臭,监测,毒性试验,有毒化合物,抗菌素。

A、Head wear ,plastron and trinket serial products with all kinds of material and novel design;finger ring and necklace serial products;foot ornament, adornment and article decoration serial products;ear ring and waist decoration products; chain ornament franchise store;decoration accessories;tie clip;mobile phone decorations ;eyeglasses ;related machinery equipments etc..

A、各类材质设计新颖的头饰、胸饰、手饰产品系列;戒指、项链系列产品;脚饰、佩戴饰和物饰系列;耳环、腰饰产品;饰品连锁加盟店;饰品配件;领带夹;手机饰品;眼镜;相关机械设备等。

CHF-associated diseases were Caroli's syndrome, polycystic kidney disease, caernous transformation of the portal ein, Joubert's syndrome, on Meyenburg complex, polydactyly, medullary sponge kidney, and pancreatic duct atrophy.

先天性肝纤维化相关的疾病包括Caroli's综合症、多囊肾、海绵状门静脉形成、Joubert's综合症,on Meyenburg 复合物,多指趾畸形,髓状海绵样肾,胰管萎缩。2例发展成胆管癌。

Next, BALB/c mice were orally infected with Y. pseudotuberculosis and its ompR mutant to study metabonomic variation of different intestine-associated organs after 24 h infection. The results showed that a majority of metabolites were decreased during this acid adaptation process, but phosphoenolpyruvate, 5'-GMP, 5'-AMP, 5'-UMP, GDP/GTP of wild type strain and lactate, Tyr, phosphoenolpyruvate, 5'-AMP、GDP/GTP of the ompR mutant strain were increased.

为了探讨病原细菌感染动物后特别是在酸性环境下代谢物的变化规律,首先在体外模拟假结核耶尔森氏菌及其全局性调控因子OmpR突变株在适应酸性环境时所引起的菌体自身的代谢变化,然后通过BALB/c小鼠口服感染耶尔森氏菌,研究小鼠感染病原细菌24h后各种相关器官的代谢变化。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。