相关法

For example, HLA associations with vitiligo include HLA-DR4 in Caucasian Americans, DR4 and DQW3 in black Americans, DR4 and DR6 in a Dutch caucasoid population, DR7 and DQW3 in north Italians, DR7 in Omanis, DR1 in Hungarians, DRW12 in north Germans, and DW7 in Slovakians. Negative association of DR1, DR3 and DR52 were observed in north Italians. With the DNA typing of HLA, it was found the frequencies of HLA-DRB1〓0701, DQB1〓0201, DPB1〓1601 alleles were significantly increased in Slovak patients and DRB4〓0101 were increased in Dutch patients.

此后发现美国黑人与DR4、DQW3明显相关；匈牙利、德国和斯洛伐克患者中，HLA-DR1、DRW12和DW7抗原频率分别升高；HLA-DR7与意大利患者明显相关，而DR1、DR3则明显降低；荷兰患者中HLA-DR4和DR6抗原频率明显升高，而DR3明显降低；HLA-DR7与阿曼人明显相关，而肢端型又较局限型明显升高。90年代后，DNA分型法用于HLA的研究，发现斯洛伐克患者与HLA-DRB1〓0701、DQB1〓0201、DPB1〓1601明显相关；荷兰患者的DRB1〓0400，〓0901，〓1300、DRB4〓0101、DQA1〓0301、DQB1〓0302，〓0303基因频率升高，而DRB1〓1100、DQA1〓0501降低，但经校正后，仅DRB4〓0101有显著意义。

In addition, multivariate statistics simulation models (the correlation and regression analysis) are employed to account for the relationship between the cultivated land changes and soci-economic factors.

在相关分析的基础上，运用相关分析法和多元回归法分析耕地面积变化和社会经济因素变化的关系，建立区域土地利用／覆被变化的驱动力模型，提出各个土地利用区的主导驱动力分类。

Results The testing results showed that this method with 100% specificity and sensitivity in 300 positive and 100 negative samples of mammalian sera respectively. However, the positive serum samples of Francisella tularensis, yersinia pseudotuberculosis and yersinia enterocolitis could not be deceted by dipstick of ICA.

结果 间接血凝法检测阳性300份血清标本，胶体金法检测全部为阳性，间接血凝法检测阴性100份血清标本，胶体金法检测全部为阴性，两种方法符合率100％；并且鼠疫胶体金法快速诊断盒不与假结核耶尔森氏菌等相关细菌的免疫血清发生交叉反应。

The thinking of this method as follows:Firstly,using single evaluation methods to evaluate.If the evaluation results aren\'t same,the Spearman rank correlation coefficient is used to calculate the correlation among the results of single evaluation methods.Based on the results,the traditional combined evaluation methods are used to combine the results of the single evaluation method.If the results of the upper combined methods are not same. Here the combined method evaluation indexes,such as Spearman rank correlation coefficient,correlation degree,the max deviation,average deviation,are used to evaluate the upper four combined evaluation methods.The entropy method is used to calculate the weights,and then get the final evaluation result.

首先，采用单一综合评价方法进行评价，对于不一致的评价结果，通过Spearman等级相关系数验证各单一评价方法间是否具有相关性；(来源：4dbdABbbC论文网www.abclunwen.com)对存在相关性的单一评价方法采用组合评价法对其结果进行组合，若所得评价结果仍不一致，采用Spearman等级相关系数、关联度、最大偏差、平均偏差对组合评价方法的组合效果进行评价，最后运用熵权法对上述组合评价进一步组合得到最终评价结果。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Methods M. penetrans was cultured and its LAMPs were extracted. Mouse macrophages were stimulated with M. penetrans LAMPs to analyze the production of nitric oxide. Cell prolification was detected in M. penetrans LAMPs-stimulated mouse macrophages by MTT assay.

用SP-4培养基培养穿透支原体并提取其脂质相关膜蛋白，用提取的脂质相关膜蛋白刺激小鼠巨噬细胞，以ELISA法检测NO的产生，并用MTT法检测其对小鼠巨噬细胞增殖的影响。

The marked price comment method, the unmarked price comment method, the mainstream reasonable low price comment method and the compositive comment method are unable to achieve the mutual recognition and to resolve the questions of the development of our country construction project bid. Though the lowest price comment method of western nation is accord with the rules of the market economy and have the scientific character and the practice feasibility,the method is not fit of the development actuality of the market economy and the development moment of the architecture industry, and unallowed to put in practice.

不论是早先的有标底中标法，还是后来的无标底中标法，甚至于目前最为主流的合理低价中标法和综合评标法都无法在理论上达成共识并在实践中彻底解决我国建设工程招投标的发展问题，而西方国家普遍通行的最低价中标法虽然符合市场经济竞争规律并具备理论的科学性和实践的可行性，但却因不适合具有中国特色的社会主义市场经济的发展现状和我国相对落后的建筑业及其相关领域的发展状况而处处受制，频频&流产&，以至于遭遇&严禁使用&的尴尬局面。

The compensation system of first-line workers of state-own coal enterprise was redesigned under the direction of compens

结合相关薪酬理论，对国有煤炭企业一线员工的薪酬体系进行了全新设计，构建了由基本薪酬、效率薪酬、安全薪酬及福利薪酬四大部分组成的以激励为导向的&绩效薪酬体系&，详细论述了各大组成部分的设计方法和设计步骤：综合考虑企业生存状况和社会收入状况，采用以劳动定额和社会平均收入相互平衡的方法，设计出一线员工的基本薪酬模块；提出了用专家评议法确定施工难度等级指标体系的相关权重、用综合模糊评价法来确定作业难度等级、把团队人力资源要素与作业区域的施工难度状况紧密结合起来测算团队业绩，构建出体现团队业绩和贡献的效率薪酬模块；构建了以&安全抵押&制度为核心，以月度安全收入、季度安全收入、年度安全收入为框架，形成了短期、中期与长期安全收入相结合的安全薪酬模块；结合国有煤炭企业一线员工来源复杂、身份复杂、需求不同的现状，采用了员工参与性和自决性极强的&自助式&福利薪酬模式。

Through relevant researches, the most commonly adopted way to establish visual beauty anticipation model is Scenic Beauty Estimation, which is a psychophysical paradigm. However, due to the fact that this model does not consider the ecological vegetable when selecting factors, it is not adoptable for the purposes of this thesis. Moreover, the application of the "multiple linear regression analysis" does not effectively show the relationship between the ecology and aesthesis of the vegetable.

经由相关研究得知，建立植栽之景观美质预测模型，最常运用心理物理模式中的景观美质评估法，不过其因子的选定并非以植栽之生态性为考量因素，与本研究希望藉由植栽之生态性与景观美质之定量相关建立预测模式不符；另外应用多元线性回归分析法，并无法有效反应上述两者之关系。

The instruments the author used in the experiment are literature review, interviews, questionnaires and case study. Start from the point of view of strengthening amateur sportsmen's training method in view of the 400-meters sportsmen who haven't reached level 2 target in school PE teaching and amateur training according to the successful experience and long time of practice of some qualified 400 meters trainers and some out standing PE teachers as well as the accumulation of the related literature review the author construct theoretically the model of training 400 meters male dash with the target of improving the speed of 400 meters dash. That is to say from the following 4 aspics, speed index,speed endurance index,aerobic endurance index,strength index.Listing the relative theory of mode training by analyzing and comparing the results got by the subjects both before and after the experiment the author tries to find out a suitable final index mode for specific training .

本文运用文献资料法、访谈法、问卷调查和实验等方法，从重视基层业余水平田径运动员的训练、加强对业余水平运动员训练方法的研究为出发点，针对学校体育教学及业余训练中水平一般且未达到国家二级锻炼标准的400m跑运动员，依据国内400m跑专项高水平教练员、优秀体育教师多年训练实践的成功经验以及相关文献资料的积累，以影响400m跑专项成绩提高的主要内容指标，即速度指标、速度耐力指标、有氧耐力指标以及力量指标四个方面为切入点，从理论上初步对男子400m跑二级运动员专项训练的指标模式进行构建，并运用模式训练相关理论原理，通过实验前、后运动员成绩的对比分析，寻求符合其专项训练的最终指标模式。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了