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The cutoff value of E2/E6, used to distinguish the pure episomal form from a mixed form of episomal and integrated HPV16 DNA, was 0.81 in the multiplex real-time PCR test. c) HPV16 existed as episomes in most of the CIN Ⅰ lesions, and 42.9% of them had mixed episomal/integrated HPV16. The integrated form of HPV16 constituted the majority in most of the CIN Ⅱand CIN Ⅲ cases. In squamous cervical carcinomas, the HPV16 was mostly integrated into the host chromosome. d) The rate of HPV16 integration was associated with the degree of cervical lesions and the rate increased with the progression of cervical disease, e) The rate of pure integrated HPV16 in stage Ⅱ and Ⅲ(88%) was higher than that in stage Ⅰ(33.3%). Conclusions: a) Multiplex real-time PCR is a rapid and sensitive method for detection of the integration state of HPV16 DNA. b) The integration of HPV16 DNA is a very early event in the development of cervical cancer and may act as an activation mechanism for progression from preinvasive to invasive cervical cancer, c) The pure integrated HPV16 in cervical cancer may be a useful prognostic indicator.

HPV—16E2、E6标准曲线相关系数均为1.00,扩增效率均在95%以上:2)确定0,81作为多重实时PCR区分游离型和混合型HPV-16的界值、3)CINⅠ中HPV—16大多以游离方式存在,但仍有整合的发生(42,9%);CINⅡ和CINⅢ中HPV—16以混合型为主:浸润癌中以整合型HPV—16为主。4)HPV-16整合发生率与不同程度的宫颈病变有关,并且随着宫颈病变级别的升高HPV-16整合发生率呈上升趋势-5)Ⅱ+Ⅲ期宫颈癌中整合型HPV—16所占比例显著高于Ⅰ期宫颈癌结论:1)多重实时定量PCR是一种敏感性高、简便快速的HPV—16病毒存在状态的检测方法2)HPV-16整合是宫颈癌变的早期事件,与宫颈癌前病变的进展有关。3)宫颈癌中整合型HPV—16的发生可能具有预后价值

METHODS:The dissolution curves of nicrandil-ethylcellulose solid dispersion granules and tablets were measured. The release data were fitt ed with three model equations. The release kinetics and release mechanisms of g ranules and tablets were judged by the sequence of correlation coefficients of t hree fitting equatuions.

测定尼可地尔-乙基纤维素固体分散物颗粒及片剂的释放曲线,并用3种模型方程--零级动力学方程、一级动力学方程、Higuchi模型方程进行线性拟合,根据所得方程的相关系数的大小顺序,确定颗粒及片剂的释放动力学及释放机制。

In the process of the design of gas detector, it is usually by the ways of refer to the energy response graphic of the working gas and the material for the chamber to choose the working gas and the material for the chamber.

而在一般的气体辐射监测器的设计中,工作气体及壁材的选取基本上都是通过查阅相关气体及壁材能量响应曲线的工程图表,以确定具体的工作气体及壁材。

The reservoir pressure has an exponential correlation with cumulative production and gas wells can be divided into three types by gas-producing curves.

研究发现红台2块气田地层压力与累计产气量呈指数相关,根据采气曲线特征,可将气井分为相对稳产型、递减较快型和无稳产期型三种类型。

Based on the detailed analyzing of impact system working process, the differential eguation of the system was got, and the mathematical model of the hydraulic impact system was builted, on basis of the mathematical model, By using of dynamic simulation function of Matlab ,the author has built the simulation model of the impact system based on software Simulink and run it after inputting the parameter ,the dynamic graph curve of impact velocity and presser of oil and position of knife rest were got, and the dynamic characteristic of impact system was analyzed,the simulated results are accorded with the fact,and some rational parameters can be found.

在对冲击式采煤机液压冲击系统工作过程进行详细分析的基础上,运用微分方程建模方法,建立了液压冲击系统的数学模型即系统的动态方程,并在该数学模型的基础上,采用Matlab中的Simulink动态仿真功能,建立了基于Simulink的冲击系统仿真模型,通过给定冲击式采煤机冲击系统的相关参数,对该系统进行仿真,得到了冲击式采煤机工作过程中冲击速度和油液压力以及刀架位移的变化曲线,进而对冲击系统的动态特性进行了分析,结果与实际情况较为吻合,并由此得出某些合理的参数。

In this paper, the mechanical property parameters of the adhesive were gotten by tensile test on standard sample at first. Then the single lap joint test of tensile shearing strength was designed and the loading-displacement curve including the cracking load was obtained. The experimental research provides the certain basis for the further research and application on the automotive structural adhesive.

本文首先制作了结构胶粘剂标准试样,通过拉伸实验测试了胶粘剂的相关力学性能参数;然后设计了单搭接拉伸剪切强度试验,测得该类型试件的载荷-位移曲线以及破坏载荷等重要数据,为今后车用结构胶合技术的进一步研究应用提供一定的实验依据。

The results show that present model can effectively describe the scale-dependent long-tailed phenomena of experimental results and be used as general filtration model.

研究结果表明该模型能很好地刻画实验曲线中出现的与介质尺度相关的拖尾现象,可作为更一般的过滤模型。

The subjects of this study are 11 male high school tuggers(16.8±0.99 year old, 171.59±3.18cm, 70.14±2.65kg, trained for more than 2 years and having used the plyometric strength training machine of Tug-of-war for 8 weeks). We collected the related parameters of Defend Fast Break, and Attack Fast Break before and after the training for our study and tested it by " pair-data sample t test ".

研究的对象为11名优秀高中男子拔河选手,平均年龄16.8 ± 0.99岁;身高171.59 ± 3.18 公分;体重70.14 ± 2.65公斤;训练经历二年以上,并利用拔河增强式肌力训练机进行为期8周的增强式肌力训练,以拉力计(采样频率为600Hz;采样时间为5秒)收集个人分别在训练前、后防御起动步与攻击起动步之的拉力曲线相关参数,作为本研究之研究范围,并以成对样本t考验进行考验(α=。05)。

In the same direction, the focus on the crowns of their head, then a group portrayed face on to those in profile, a one-child family,in a tondo composition, with father filming and mother and child grouped in harmony, or a swanky duo dressed up to party, The slightly unreal appearance of these standardized groups is intensified by thinly applied colors, where the plastic effect results more from a masterful application of color rather than from a concern with the forms of the body.

在稍近的一些作品中,画面开始复杂,四个女人站成一排,显示出美丽的发型或者优美的脸部曲线;圆形构图中的三口之家,父亲用录像机拍摄着妻儿;或者还有在聚会上穿着入时的人们。而在这样一些标准的美丽的图像有着一些稀薄的颜色,与其说它们是跟画面相关颜色倒不如说它们像一种塑料一样覆盖在这些图像上。

Beijing Fatty Chicken, Langshan Chicken and Small-tailed Han Sheep were selected as experimental material. Fibroblast library was constructed through tissue confluency culture and cell freeze technology. Growth dynamics, genetic stability and contamination of of cross breeds etc.were detected through growth curve, chromosome analysis, isoenzyme analysis etc. Cloning and sequence of gene GPAT of Beijing Fatty Chicken was analyzed by cloning technology and software related.

本论文以北京油鸡、狼山鸡、小尾寒羊为研究对象,采用组织块贴壁培养法和细胞冷冻技术构建成纤维细胞库,采用生长曲线、染色体分析、同工酶分析等方法对细胞生长动态、遗传稳定性、种间交叉污染等进行检测;采用基因克隆技术和相关分析软件对北京油鸡GPAT基因进行克隆和序列分析。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。