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Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上,开展了如下未见文献报道的创新性的研究工作:(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究,建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法,与以前的报道相比,灵敏度和分析时间均有很大改善;(2)使用荧光素钠作为背景荧光试剂,建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法,扩大了激光诱导荧光检测的应用范围,对难衍生化合物的分离分析提供了一种新思路和新途径;(3)以荧光素钠作为背景荧光试剂,建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法;(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物,为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Theobserved strain is 10~2 times as local point strain at the same observed stress.This indicates that macroscopical symmetrical rock is quasi-brittle materialwith microcosmic heterogeneousness and its macroscopical mechanicalbehavior is the results of reciprocity and magnification between microcosmiccharacteristic, not simple sum.(4) The degree of irregularity of strain increases with the number of cycle.But degree of irregularity of transverse strain fluctuates larger at the samecycle. Moreover degree of irregularity of transverse strain is larger than that ofaxial strain. This shows that deformation and failure of rock is process ofevolvement of small crack. Small crack evolves from even and out-of-orderstatus to heterogeneous and ordinal status.(5) Ultrasonic speed decreases with the number of cycle. The decreasecourse is such: Firstly, ultrasonic speed fluctuates in a steady scope in somecycles; And then ultrasonic speed quickly decreases to a certain more smallvalue; Afterwards in succedent cycles, ultrasonic speed fluctuates about thevalue. That is to say, ultrasonic speed fluctuates and decreases at intervals ofcycles. In deformation process of rock, quantitative change and qualitativechange of its small crack evolvement carry through crosswise.(6) Strength and modulus of rock decrease with exponential function asscale of specimens increases.

同一表观应力下,局部点应变与表观应变相差可达2个数量级,表明宏观上均匀的岩石是具有细观特征的准脆性材料,其宏观力学行为是细观特征相互作用、放大的综合结果,而不是简单的叠加;(4)岩样轴向应变不均匀程度和横向应变不均匀程度随循环次数的增加渐进增大,但是在同一个循环内,横向应变离散系数随应力的变化波动较大,且同一循环级别下,横向应变不均匀程度大于轴向应变不均匀程度,表明岩石内部微裂纹的演化是岩石变形破坏的本质特征,这一演化表现为微裂纹从均匀无序分布逐渐向非均匀有序发展;(5)随循环次数的增加,所有岩样内部超声波速会出现衰减,衰减过程表现为在某几次循环内超声波速在某一相对范围内波动,然后急剧减小到某一较小值,在随后的循环内,超声波速又会保持在这一较小值附近波动,即超声波速表现为波动和急剧减小间隔形式衰减,表明从宏观看来平稳的岩石变形过程,内部结构演化过程(微裂纹发展及其相互作用)却表现为均匀量变和突发质变不断交叉进行的现象;(6)岩石强度和弹性模量随尺度增大呈指数函数规律减小。

This is generally defined as being identical in the length to the width of the footing in the direction normal to the analytical plane. However, there is little knowledge about whether the results obtained from the historic method analysis are overestimated or underestimated, comparing with those obtained from the applicable values. So in this paper a new methodology considering the soil-pile dynamic interaction for sea warf has been imposed.

在总结理论的基础上,指出土-结构动力相互作用目前的发展水平与工程实用之间仍存在相当大的差距,理论与实际之间也还有距离,这是由于土-结构动力相互作用问题本身的复杂性,不同的计算方法都引进了一些假设和进行了不同程度的简化,以致不同方法给出的结构和地基反应存在有很大的差别,加之计算工作量大,试验验证也比较困难,所以在实际的抗震设计中考虑桩-土共同作用是有很大的研究意义的。

There have been massive researches on improving the properties of protein in recent years, this article emphatically begin with the interaction between tea polyphenol and protein (egg albumen protein, soybean protein isolated, whey protein concentrated, bovine serum albumen), study their functional rules and that under the changed food processing environment (the pH value, salt ion concentration, sucrose density), and then apply the interaction rules into two different kinds of cakes for improving the technology of their production, aiming for provide the new mentality and method on improving the properties of protein as well as speed up the application of tea polyphenol.

本文着重从茶多酚与蛋白质的相互作用着手,研究了茶多酚及其主要单体组分对蛋清蛋白、大豆分离蛋白、乳清浓缩蛋白及牛血清蛋白的功能特性的影响规律,同时也探讨了环境因素(pH值、盐离子浓度、蔗糖浓度)与茶多酚协同作用对蛋白质这些功能特性的影响规律,并且初步应用了这种相互作用规律到两种不同工艺蛋糕的生产中来,目的是为蛋白质功能特性的改善提供新的思路和方法以及加快茶多酚在食品中的应用。

The hard segments pack firmly and form hard segment domains which bear certain degree of regularity. In polyether polyurethane zwitterionomers,variation of the amount of hydrogen bondings formed between hard and soft segmentswith the degree ionization conforms with the phase separation between the hard andsoft segments observed in wide line study.

特别是聚醚聚氨酯离聚体浓溶液中软段与硬段之间形成的氢键的数量和固体变温方法测得的样品的相分离情况完全一致,表明浓溶液中高分子链段间的相互作用和固体条件下的情况一致,可以通过浓溶液的核磁共振研究来了解链段间的相互作用,研究影响体系相分离的因素。

In order to study the effects of 3 protein-4.2 point mutations associated with HS, we have cloned four recombinant fusion proteins: GST-P4.2 (31-200) D175Y, GST-P4.2 (31-200) D175Y, GST-P4.2 (100-260) D175Y, GST-P4.2 (31-200) A142T with pointmutation, to do prokaryotic expression, and tested their interactions with ankyrin by Far-Western blot and Pull-down assay, but there were no evidences to show these mutated proteins can bind to ankyrin except GST-P4.2 (31-200) A142T.

于是我们采用了点突变的方法,克隆表达了4种重组的突变蛋白GST-P4.2(31-200)D175Y、GST-P4.2(31-200)D175Y、GST-P4.2(100-260)D175Y,GST-P4.2(31-200)A142T,发现第175位氨基酸残基的突变使得相应的重组蛋白均不能与ankyrin直接相互作用,而第142位氨基酸残基的突变对P4.2与ankyrin之间的相互作用没有影响,这说明第175位氨基酸对于P4.2与ankyrin的结合是非常重要的,很可能是一个关键位点。

A lot of experiments were finished to gain musks two components phase equilibrium data of many solvents,and these data were used to fit UNIFAC model's interaction parameter to endow UNIFAC model modified with better prediction function.

通过试验测定了合成硝基麝香二元体系的固液平衡数据,回归并修正了 UNIFAC 模型的相互作用参数,使 UNIFAC 模型具有更高的预测功能;为了评价修正后的 UNIFAC 模型对合成硝基麝香三元体系的预测功能,利用试验测定的溶解度数据和修正后的 UNIFAC 模型计算值进行了比较,结果表明相互作用参数的修正是理想的。

The introduction of quark delocalization can be viewed as taking into account either the contribution of excited configurations or the distortion of individual baryon due to mutual interaction, its characteristic is to allow the six-quark system to choose the favorable configuration by its own dynamics automatically; Various quark models extended linear or quadratic confinement potential for 3q system to 6q system directly. Due to the nonlinearity of QCD, quark-quark interaction in color singlet may be different from that in non-color singlet. For example, the contribution of three-gluon exchange to one baryon is different from that to two baryon singlets, and so on.

引入夸克蜕定域效应,既可以看作是考虑了具有较高能量的激发组态的贡献,也可以看作是考虑了重子不碰撞过程中发生了畸变,其特点是让夸克体系自动选取最有利的组态;以往的QCD模型总是将哈密顿量直接从单重子系统推广到双重子系统,但QCD理论是一个非线性理论,处于色单态的两个夸克间的相互作用可能不同于处于非色单态的两夸克间的相互作用。

It is included the energy spectrum and angular distribution of hot electrons generated by ultrashort laser-solid interaction, the effect to electron behavior of electrical conductivity of target material, the hot electron activities and plasma characteristics in various plasma scale length, the rule of electron movement related to the high harmonic generation in gas, the excelled calculation about the condition of high harmonic generation, the study of electron spectrum and ion spectrum generated by Coulomb explosion in laser cluster interaction.

其中包括超短脉冲激光与固体靶作用中超热电子的能谱和角度分布,靶材料的电导率对电子行为的影响以及在不同的密度标长条件下超热电子和与电子相关的等离子体特征;超短脉冲激光与气体靶相互作用中与高次谐波相关的电子的具体运动规律和产生短波长高次谐波的优化条件;超短脉冲激光与团簇原子相互作用中通过对电子的隧道电离而引起的库仑爆炸等。

By applying the sinusoidal wave mode to simulate the rugged surface of bridge deck,accounting for vehicle-bridge interaction and using Euler-Bernoulli beam theory, a coupling vibration model of vehicle-bridge system was developed. The model was solved by mode analyzing method and Runge-Kutta method, and the dynamic response and the resonance curve of the bridge were obtained. It is found that there are two resonance regions, one represents the main resonance while the other the minor resonance, in the reson...

通过用正弦波形模拟桥面的不平和考虑移动车辆-桥梁间的相互作用,在Euler_Bernoulli梁理论的基础上建立了一种车桥系统的耦合振动模型·利用模态分析法和Runge_Kutta法对模型进行数值求解,获得了车桥系统耦合振动的动态响应和共振曲线·发现车桥耦合振动的共振曲线中存在两个共振区域,一个反映主共振而另一个反映次共振·讨论了桥面不平、桥梁振型和车辆间的相互作用对系统振动的影响·数值结果表明,这些参数对系统振动的影响很大,桥面不平和振型对车桥系统耦合振动的影响不能忽略,设计车速应该远离临界车速

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。