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METHODS: The rib trabeculae were resected and broken, trypsinizated and washed completely by PBS. Bone surface and non-adhesive floating cells in cleaning fluid were observed with inverted microscope. Rib trabeculae was washed by DMEM culture medium once, and cultured in culture bottle. The culture liquid was replaced by new one once a week. The osteoblast was moved from the sclerite a week later. The cells were fused monolayer and could be subcultured 4 to 6 weeks later.

切下人肋骨骨小梁剪碎,胰酶消化后以PBS彻底冲洗直至于倒置显微镜下观察骨片表面及清洗液中无粘着及漂浮的细胞,以DMEM完全培养液清洗骨小梁1次,移入培养瓶内培养,每周更换培养液1次,1周后可见成骨细胞自骨片移出,4~6周细胞融合成单层可作传代培养。

To take positive and negative pressure valve to open three valves, screw pine differential pressure transmitter body and sewage screws for the sewage, the sewage completed tighten the screws;⑦ observe the instructions of instruments, until the output of a normal, if the regulating system, and auto-manual switch.

开启三阀组正负取压阀,拧松差压变送器本体上排污螺丝进行排污,排污完成拧紧螺丝;⑦观察现场指示仪表,直至输出正常,若是调节系统,将手动切换与自动。

To take positive and negative pressure valve to open three valves screw pine differential pressure transmitter body and sewage screws for the sewage the sewage completed tighten the screws;⑦ observe the instructions of instruments until the output of a normal if the regulating system and auto-manual switch.

开启三阀组正负取压阀,拧松差压变送器本体上排污螺丝停止排污,排污完成拧紧螺丝;⑦观察现场指示仪(guan1 cha2 xian4 chang3 zhi3 shi4 yi2)表,直至输出正常,若是调节系统,将手动切换与自动。

The work is a performative investigation into the everyday living situation of the place of the Biennial as well as a sculpturally investigation into the air-space which is defined as the negative space of the place.

我住在这里照常作息,但我用透明胶袋把我呼吸的空气都储存下来,直至这些储有我空气的胶袋填满了整间屋为止,过程一共用了十天的时间。

We shall fight him by land, we shall fight him by sea, we shall fight him in the air, until, with God's help, we have rid the earth of his shadow and liberated its people from his yoke.

我们将在陆地上与他作战,我们将在海洋上与他作战,我们将在天空中与他作战,直至在上帝的帮助下,总地球上彻底驱除他的阴影,从他的枷锁中解救其人民。

We will fight him by land, we shall fight him by sea, we shall fight him in the air, until, with god's help, we have rid the earth of his shadow and liberated its people from his yoke.

8226;我们将在陆地上与他作战,我们将与海上与他作战,我们将在天空与他作战,直至在上帝的帮助下,从地球上彻底驱除他的阴影,从他的枷锁中解救其人民。

Up to the Qing Dynasty, Great Wall and to become unified, the old leader, had lost their military defense role as emperor, literati great place to view the sea Range Rover.

直至清代,长城内外成为一统,老龙头从此失去了军事防御作用,成为帝王将相、文人墨客观海览胜的绝佳去处。

The sexual maturity, in the muscular layer of residual undifferentiated mesenchymal cells and smooth muscle cells in the immature, estrogen, progesterone cycle under its own role continuity (self-perpetuating) proliferation, differentiation and mast process, repeated in a long time, until the formation of fibroids.

进入性成熟期后,残存于肌层的未分化间叶细胞和未成熟的平滑肌细胞,在雌、孕激素周期作用下出现自身连续性( self-perpetuating)增殖、分化及肥大过程,在长时间内反复进行,直至形成肌瘤。

Result After the ground-source heat pump system ran continuously for a period of time, the temperature field in the range with the semidiameter of 0.8 m around the underground pipeline showed intensive changes. The changes of the temperature field in the area outside this region became smaller till it was close to the original temperature of soil. The original temperature field in the soil of shallow ground surface showed an exponential correlation with the depth. When the depth reached to certain extent, the soil basically maintained the constant-temperature state and it was 1~2 ℃ higher than the annual average temperature.

结果]地源热泵系统连续运行一段时间后,地埋管周围半径为0.8m的范围内温度场会呈现强烈的变化,该区域以外的地方温度变化越来越小,直至接近于土壤的原始温度;浅层地表土壤原始温度场分布与深度呈指数关系,当深度达到一定程度后,土壤基本上维持恒温状态,比当地年平均气温高1~2℃。

The post-operational patient on liquid or semiliquid diet injected insulin subcutaneously before three meals,applied inˉsulin according to blood sugar level until end of perioperative period.

目的 探讨糖尿病对耳鼻咽喉—头颈外科患者手术后并发症的影响以及糖尿病患者的围手术期治疗方法对51例接受耳鼻咽喉及头颈部不同类型手术的糖尿病患者,观察其围手术期并发症,并于术 3天改三餐前0.5h皮下注射速效胰岛素治疗,根据血糖的测定结果,开始剂量约为4~8U;对术后禁食需要补液的患者,按葡萄糖3~4g/胰岛素1U的比例给药,并每日监测血糖指标;术后进流质或半流质饮食的患者,于三餐前皮下注射胰岛素,按血糖测定情况给药,直至围手术期结束。

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