直接插入

In addition, the plasmid has been shown to remain unintegrated, or extrachromosomal. Thus, plasmid-based gene therapy by intramuscular injection may be safe, cost-effective and have high patient compliance due to the acceptability of intramuscular injection.

在所有这些转移方法中，裸DNA直接注射法具有：安全可靠（不会引起炎症反应和导致插入突变）、制作工艺简单、操纵简便（与传统的注射方法非常相近）、可重复注射等优点。

Deprived of their industrial use, dedicated to activities closely related to the new vocation of the places,(a marina for the basin of the citadel for example or a nautical base at the end of the Holy district Nicolas), These places will be domesticated thanks to the insertion of a strong vegetable structure adopting the volumetry of the buildings, their linearity, their functional logic, their direct character and without affeteries.

剥夺工业用途，致力于活动密切相关，新的使命的地方，（一个码头的盆地堡垒例如或海里基地年底罗马区尼古拉），这些地方将被驯化感谢插入一个强有力的蔬菜结构采用体积建筑物，其线性度，其功能的逻辑，他们的直接性质和affeteries 。

There are two synthetic methods for the two carboxylates starting from pyrrole,(1) the carbonylation of pyrrole using trichloroacetyl chloride and then the esterification reaction in methanol, or 2-MPC can be directly synthesized from pyrrole and methyl chloroformate,(2) the insertation of CO2 to pyrrole for the corresponding 1-pyrrole carboxylic acid or 2-pyrrole carboxylic acid and then the esterification reaction in methanol.

两种吡咯甲酸甲酯的合成从吡咯为起始原料有两种方法：（1）使用酰氯将吡咯酰化，然后与甲醇完成酯化反应，或者吡咯与氯甲酸甲酯直接合成吡咯甲酸甲酯；（2）先将吡咯通过CO2插入反应得到1-吡咯甲酸或2-吡咯甲酸，然后与甲醇反应完成酯化。

A copyright was written before me before, collect fees the article that station and free station are faced with heaven and earth, although inserted the Ad that publicizes octave stationmaster net among them, but, because that is me,do station of 3 years of films to realize the thing that give, it is the experience that the summary in I and other center communication comes out, so, the center that it has implication with copyright to be being done people have very good directiveness, after that article is given out, the QQ that 20 no less than stationmaster add me directly seeks advice from relevant matters concerned.

我前面写了一篇版权面前，收费站和免费站面临天壤的文章，尽管其中插入了宣传八度站长网的ad，但是，由于那是我做三年电影站悟出的东西，是我和别的站长交流中总结出来的经验，所以，它对正在做与版权有瓜葛的站长们具有很好的指导性，那篇文章发出后，有不下20个站长直接加我的QQ咨询相关事宜。

Study on different methods of iodomethane s preparation;2. The carbonyl group of DMC is formed by CO_2 insertion, and the two methyl groups of DMC are from methanol and iodomethane , respectively.

结果表明，固体碱碳酸钾催化剂直接参与了DMC的合成过程，DMC分子中的羰基由CO2插入甲醇后形成，两个甲基分别来源于甲醇和碘甲烷。

DLT insertionposition was immediately confirmed with fiberoptic bronchoscopyafter direct laryngoscopic placement and after lateral positioning.

用直接喉镜下放置好和侧卧位后，立即用纤支镜确认 DLT 的插入位置。

Then we transformed mTn-lacZ/LEU2 transposon library into the YES2MTM1 strain. Transformants lost the plasmid overexpressing MTM1 after 5-Fluoroorotic acid treatment. We picked up the yeast strains able to grow on nonfermentable carbon source with MTM1 deletion and some transposon insertion and identified the insertion sites.

因MTM1基因的缺失造成的损伤不可逆，直接转入文库无法筛选得到MTM1 基因缺失表型相关基因，本研究利用外源MTM1基因菌株和mTn-lacZ/LEU2酿酒酵母转座子文库进行筛选，寻找能挽救mtm1突变体生长缺陷的转座子插入位点。

The reaction of cyclic b-keto esters with CF3CO2ZnCH2I provides the corresponding ring-expanded products with the advantage of mild condition in moderate to good yields. A simple and efficient procedure has been developed for a variety of 1,3-diketones converted into the corresponding 1,4-diketones and trans-1,2-disubstituted cyclopropanols in high yields. In conclusion, this new type of organozinc reagents has been developed for many useful reaction in organic synthesis.

利用有机锌试剂所具有的双重特性，在新型的Baylis-Hillman反应中，可以直接与炔基酮和醛反应一锅制得多官能团的三取代的顺式烯；在b-酮酯化合物的扩环反应中，取得了重要进展，得到了增加一个碳的扩环产物，&一步法&合成，反应条件温和、环境友好；在1，3-二酮分子中成功地插入1个碳，合成出1，4-二酮和环丙醇，为现代有机合成提供了新的合成方法，具有良好的应用前景。

With your right hand at the back of the fabric, in s ert the needle in the lower right corner of the Aida s quare, directly below the top of the half s titch.

随着你在织物回右手，插入在阿依达广场右下角针，直接低于半针的顶部。

Check to s ee that the needle i s in s erted directly in the s pace in the corner of the Aida s quare.

请检查该针插入在广场角落空间阿伊达直接。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。