目的

The Omp25 gene of wapiti's Brucella abortus was amplified by RT-PCR and sequenced, Then the interesting gene was subcloned into pET28a for expression. The results showed that the full length of Omp25 gene was 642bp, which encoded 213 amino acids.

运用RT-PCR技术从马鹿布氏杆菌分离株中扩增出Omp25基因，将其克隆入pMD18-T中进行核苷酸序列测定和遗传变异分析，并将目的基因亚克隆到大肠杆菌表达载体pET28a中诱导表达。

In response to the above-mentioned, there are two objectives of this research.

为了确认其可行性，本研究的研究目的有二。

The main purpose of this thesis is to provide some references for above-mentioned tugboat enterprise by academic research.

本文的主要目的是通过理论研究为上述轮驳企业船队的发展规划提供一定的参考依据。

Objective To study the value of ABR in diagnosis of acoustic meuroma.

目的 探讨听性脑干反应在听神经瘤诊断上的实际应用价值。

Objective To observe the changes of DPOAE and ABR in animal model of cerebral concussion of Wistar rats.

目的 探讨Wistar大鼠脑震荡模型双耳DPOAE及ABR改变的特点。

Objective To investigate the relation between ABR and 40 Hz AERP and to discuss its importance to hearing recovery.

目的 探讨 ABR和40Hz AERP测试的相关性以及聋儿听力康复的重要性。

Objective: To investigate the efficacy of MEBO in treating wounds after skin abrasion.

目的：探讨MEBO应用在皮肤磨削术中修复创面的临床效果。

Objective To investigate abrasive wear of composite resins in vitro.

目的 评价牙科复合树脂材料的抗磨损性。

We must keep abreast of the times; that is the purpose of our reform.

我们要赶上时代，这是改革要达到的目的。

Objective:To investigate the etiology,early diagnosis and treatment of placental abruption.

目的：探讨胎盘早剥的病因、早期诊断及处理方法。

The X-ray diffract was used to analyze the crystal structure of the composite. Results show that after the talc was treated with blend coupling agent, the crystal structure of talc changed, and effect Intercalation phenomenon.

通过X射线衍射分析可知，用混合偶联剂对滑石粉进行预处理后，滑石粉晶体结构发生改变，产生了插层现象，使PP／滑石粉复合材料为纳米复合材料。

An authentic Maui wedding might require the services of a 'kahu', which is a Hawaiian minister.

毛伊可能需要一个真实婚礼服务'kahu'，这是夏威夷部长。