目的

The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2 -chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

AIM: To generate random sequences that can fit the anticipated probability distribution based on the thinking of MC, METHODS: We establish a method in the IDE of visual studio by the way of Linear Congruential Method to generate random sequences.

目的：基于MC法生成符合预期概率分布的随机序列，以便应用在以后的仿真系统中目的：基于MC法生成符合预期概率分布的随机序列，以便应用在以后的仿真系统中。

It comprises two species,Dianulites hexaporites,Orbiramus grandis sp.nov.of trepostome esthonioporines,and one species,Prophyllodictya putilovensis Lavrentjeva of cryptostome ptilodictyines.

这个苔藓动物群由变口目爱沙尼亚苔虫亚目的两个种：Dianulites hexaporites，Orbiramus grandissp.nov。和隐口目翼网苔虫亚目的一个种：Prophyllodi-ctya putilovensisLavrentjeva组成。

Objective To introduce a new procedure modifying upper eyelid's dermatocele and excessive fullness.Methods In this procedure,we design incision at sub-eyebrow but not up-eyelash to do upper eyelid blepharoplasty.Using this approach,we smooth the wrinkles ion upper eyelids,excise superfluous skin and pseudo-herniated orbital fat so as to make patients youthful.Results Through this method.aging upper eyelids can be made youthful effectively and naturally.

目的 介绍治疗上睑臃肿松垂的新术式方法我们采用眉下切口而不是传统的睫毛上切口进行上睑整形，充分舒展松垂皱缩的上睑，并去除膨出的上睑眶隔脂肪及余的皮肤和轮匝肌组织，在达到改善上睑松弛使病人外貌年轻化的目的。

Taking into account the above, the purpose of this paper is to present a global architecture model (in the traditional software development sense) for any e-learning system that provides theoretical justification without losing generality; in other words, an architecture model that is valid for any type of didactical purpose.

考虑到上述情况，本文件的目的是目前全球体系结构模型（在传统的软件开发的意义）的任何电子学习系统，提供了理论理由而不概括性；换言之，一个建筑模型，是有效的对任何类型的教学目的。

The aim of this study was to assess the impact of these measures on the incidence of C. difficile and to record the associated mortality in hip fracture patients.

目的 研究的目的在于评估这些措施对于艰难梭状芽胞杆菌感染发生率的影响和并记录髋部骨折病人相应的死亡率。

The behavior purpose of advertising behavior purpose and user is very disharmonious.

广告的行为目的和用户的行为目的是非常不和谐的。

The monotypic genus Macrovalsaria previously of uncertain taxonomic position turns out to be a member of Botryosphaeriales and seems to be related to Botryosphaeria. The placement of Neopeckia and of Dothidotthia is in Dothideales and Botryosphaeriales, respectively. The genera Plowrightia and Botryosphaeria as currently defined are not monophyletic. Species of Plowrightia appear to be associated with three genera, Dothidea, Neopeckia and Sydowia; those of Botryosphaeria tested are recognized as three groups.

结果还显示，座囊菌纲中分类地位不确定的属Macrovalsaria与Botryosphaeria的关系相对接近，应该归于葡萄座腔菌目；历史上分类地位有疑问的Neopeckia和Dothidotthia两个属处于不同的目中，前者属于座囊菌目，后者为葡萄座腔菌目的成员；座囊菌目的Plowrightia属并非单系群，该属成员分别与Dothidea、Neopeckia和Sydowia三个属聚类在一起；现行的Botryosphaeria属也非单系群，其属的概念有待澄清。

The Gardner thinks the foreign language learning motive should include four aspects:The purpose a goal, the diligent degree effortful behaviors of the study, attains the wishes a desire to attain the goal of study the purpose, with the study attitude attitude.

Gardner认为，外语学习动机应包括四个方面：目的a goal，学习的努力程度effortful behaviors，达到学习目的的愿望a desire to attain the goal，和学习态度attitude。

Objectie: The objectie of this study is to inestigate the safety and effectieness of laser marsupialization of epiglottic cysts ia a transoral route.

目的：这项研究的目的是调查经口腔途径进行激光会厌囊肿造袋术的安全性和有效性。

Recall that, in the Kolmogorov axiomatization, measurable sets are the sets which have a probability or, in other words, the sets corresponding to yes/no questions that have a probabilistic answer.

延期开工的Kolmogorov宣布要衡量所有套职能有限许多坐标限制在于衡量子寅。换句话说，如果是/否的问题回答f可看的价值观念在许多最有限坐标，然后它有一个概率的答案。

The experimental results showed that the algorithm can filter singular points and remain fixity of the target edge.

实验表明：基于模糊理论的图像智能滤波方法比常规图像滤波方法运算量小，它既能去除奇异点，又能保持图像中目标的边缘不变性，具有很好的滤波效果。