目的

Recombinant ptotein made up 39.2%(pET-20b-bglA) and 33.5%(pET-28a-bglA) of the total proteins in the intracellular fraction, the solubility proportion of the enzyme up to 32.8%(pET-20b-bglA) and 40.1%(pET-28a-bglA), the activities of the enzyme were 66.8 (pET-20b-bglA) and 71.2 U/mg (pET-28a-bglA). These results showed that E. coli BL21-CodonPlus (DE3)-RIL with argU tRNA, ileY tRNA and leuW tRNA genes helped to improve expression of the enzyme through enhanced identification of the rare codons AGA/AGG, AUA and CUA. Further optimized the conditions for inducing, the solubility proportion of the enzyme was 70.6% at 16 °C and 1.2 times higher than 37 °C. The solubility proportion of the enzyme increased from 12.3% to 32.8% when IPTG concentrations dropped from 1000 μM to 25 μM. The recombinant enzyme was purified by heat treatment, DEAE Sepharose anion-exchange chromatography and TOYOPEARL HW-55F.

maritima MSB8 的-葡萄糖苷酶基因 bglA克隆至表达载体 pET-20b 和 pET-28a，构建重组质粒 pET-20b-bglA 和 pET-28a-bglA，然后转化不同大肠杆菌 E.coli 宿主，Tm-SIGlA 在 E.coli BL21-CodonPlus(DE3)-RIL 中获得高效表达，重组蛋白的表达量为 33.5%(pET-28a-bglA)和 39.2%(pET-20b-bglA)，可溶性比例为 32.8%(pET-20b-bglA)和 40.1%(pET-28a-bglA)，比酶活达 66.8 (pET-20b-bglA)和 71.2 U/mg (pET-28a-bglA)，这些结果表明，E.coli BL21-CodonPlus(DE3)-RIL 宿主带有的 argU tRNA、ileY tRNA 和 leuW tRNA 基因，分别增强对稀有密码子 AGA/AGG、AUA 和 CUA 的识别，有助于提高该酶在 E.coli 中的表达；进一步优化诱导条件，重组 E.coli BL21-CodonPlus(DE3)-RIL/pET-20b-bglA 在 37 ℃下诱导培养，IPTG 浓度由 1000 μM 降至 25 μM，目的蛋白可溶性表达由 12.3%增至 32.8%，提高 1.7 倍，16 ℃下低温诱导实现目的蛋白 70.6%的可溶性表达，较 37 ℃下诱导培养提高 1.2 倍。

Objective: cC1 gene was amplified from Cysticercus cellulosae.

目的：从猪囊尾蚴中扩增出目的基因cC1，克隆并表达。

Your medical and health data are processed on the basis of Article 7,§ 2,, of the Belgian Data Protection Act of December 8, 1992 when such processing is carried out under the supervision of a health professional and is necessary for the purposes of medical diagnosis, treatment or the management of health-care services and, on the basis Article 7,§ 2,, of the Data Protection Act of December 8, 1992 for any other purposes described in this Patient Informed Consent Form.

您的医疗及健康数据依照1992年12月8日颁布的比利时数据保护法案第7条§2款项进行处理，要求此类数据处理在医学专业人员的监督下进行，并且是为医学诊断、治疗或医疗保健服务管理之目的，以及在1992年12月8日颁布的数据保护法案第7条§2款项的基础上，为本患者知情同意书中所述的任何其他目的所必需的。

On the basis of MEBT, a new technique hypothesis of injecting MEBO under the eschar was proposed to have 6 biochemical reactions available, such as hydrolysis, enzymolysis, saponification, rancidify, lipofication, esterification of MEBO with necrotic tissue of the eschar, then to realize liquefaction and quick defluxion of necrotic tissues, This finally to play a role of regenerative action of MEBO in situ to turn potential regenerative cells into skin stem cells that would further proliferate and differentiate to form skin tissues, reaching the goal of physiologically repairing wounds.

最后提出了在烧伤湿性医疗技术正规治疗的基础上，同时进行痂下注射MEBO的新技术设想，其目的是使痂下组织尽早接受MEBO的治疗，痂下发挥MEBO的水解、酶解、皂化、酸败、脂化、酯化六大生物化学反应，促使坏死组织痂下痂外一起液化，尽快脱落；而且在痂下发挥MEBO原位再生作用，使潜能细胞转变成皮肤干细胞，干细胞进一步增殖分化形成皮肤组织，达到创面生理性修复之目的。

The purpose of flow visualization research of sections in double delta wing is to uncover the mechanism of vortex interference and structure,from this research,we can control the vortex and provide lasis for constructing numerical model.

进行双三角翼翼面流动显示研究的目的是为了揭示前、后翼脱体涡的干扰机理和详细结构，进而达到控制表面涡分离的目的，并为计算流体力学建立数学模型提供依据。

The conception of fairness and justice is based on the new summary of investigation which derive from .

公平正义的目的观，是基于法治主义而对侦查目的的新概括。

Xu Ting is very clear when taking money its are not had overdraw functional bank has more than yuan 170 only on card, the input when he takes a money for the first time takes an instruction 1000 yuan pure belong to innocently, after be being buckled 1 yuan, his much 999 yuan are belonged to civil those who go up is undeserved benefit; But, he knows perfectly well these 999 yuan not only later is not he deserved belongings will still its glom on to, and the identifying that uses an ATM is wrong, occupy promising purpose to be inputted intentionally 170 times continuously again with ill will exceed its the instruction ill will of amount of real bank savings takes a money, its behavior is belonged to trade by the contract the crime that behavior derives, accord with " criminal law " the 224th of the 5th regulation " it is a purpose with detinue, in sign, in fulfilling contract process, with property of the opposing party of other method diddle " of contract crime of fraud make important document.

许霆在取钱时很清楚其不具备透支功能的银行卡上只有170余元，他第一次取款时输入1000元取款指令纯属无意，被扣1元后，他多得的999元属于民事上的不当得利；但是，之后他不仅明知该999元不是自己应得财产仍将其据为己有，而且利用柜员机的识别错误，以恶意占有为目的又连续170次故意输入超出其实际存款数额的指令恶意取款，其行为属于由合同交易行为衍生出的刑事犯罪，符合《刑法》第224条第5项规定的"以非法占有为目的，在签订、履行合同过程中，以其他方法骗取对方当事人财物的"合同诈骗罪的构成要件。

But the latter does not serve merely historical and didactic aims.

但是，后者并不只是为历史的目的和说敎的目的而作的。

The purpose of Engels\' statement on the differential calculus is for the sake of the epistemology study.

恩格斯对微积分论述的目的也表明了他对待微积分的视角一直是为了认识论的目的。

Objective According to principle of forming primer dimer,a new cloning gene method was established.

目的根据引物二聚体形成原理，建立一种新的克隆目的基因的方法。

It was Napoleon, the immense somnambulist of this dream which had crumbled, essaying once more to advance.

那人便是拿破仑，梦游中的巨人，他还想往前走，去追寻那崩塌了的幻境。

This method alleviates the highly dependent on domain knowledge experts. The quality and efficiency of the KBS were improved.

使用这种设计方法减小了对领域知识专家的严重依赖，提高了知识系统开发的质量和效率。