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By using Roche kit as standard, the amplification efficiency of different reagents were compared by Roche Light Cycler instrument for establishing real-time PCR reaction system. The primers were designed based on signature sequence of chromosomal DNA fragment and caf 1 gene in plasmid pMT1 of Y.pestis. And sensitivity and specificity assay were done with serial diluted Y. pestis EV76 strain and other bacterias. Then 275 Y.

采用SYBRGreenI随机掺入法,以Roche试剂为标准,比较两公司的DNA聚合酶,用鼠疫菌310 0 4建立荧光PCR反应体系;针对鼠疫耶尔森氏菌特异的染色体标识序列和质粒上F1抗原基因设计引物,检测其灵敏度和特异性,以盲测试验进行验证;在此基础上鉴定2 75株鼠疫耶尔森氏菌,并测试该法检测脏器污染模拟标本的灵敏度。

This is the birth of the cylinder musical box.

这就是滚筒音乐的诞生。

Using the Hilbert transform envelope demodulation,analyzes the free damped torsion vibration signal which produced by the box of liquid milk and calculates the damping coefficient of system,compares it with standard sample to judge the quality of product.

采用Hilbert变换包络解调方法,对液态奶产生的自由衰减扭转振动信号进行分析计算,求出系统的阻尼系数,并和标准样本比较以判断产品质量的好坏。

There was Marilyn Monroe, but there was no place for Dorothy Dandridge, a black woman who was *y, who wouldn't allow herself to be put in a box.

会有马丽莲梦露,但不会再有多罗希,一个性感的黑人女孩,不允许人们将她放在礼物里。

The black box test is by user's angle, embarks from the data-in and output data's corresponding relationships carries on the test.

测试是以用户的角度,从输入数据与输出数据的对应关系出发进行测试的。

Dealing with the box model can be such a pain, especially if you're dealing with percentages.

处理模型时有这种痛苦,尤其是处理百分比。

It discovers that the cooling effect of the improved decalescence boxes is better than the primary, and attains the goal.

发现经过改进之后的吸热的冷却效果明显优于改进之前的和风冷的冷却效果,达到了预期的目的。

One set of space ship decoration with stamp, first day cover, certificate and case

神州五号收藏套装包括:金属模型摆设一尊,邮票及首日封各一套,收藏证书及

The activity of lactic dehydrogenase in the tissue culture medium was measured by a LDH kit.

使用乳酸脱氢酶试剂,测量组织培养液中乳酸脱氢酶活性的动态变化。

This is a wooden music box. It's very pretty and delicately painted by hand.

这是一个很漂亮做工很精细的木质音乐

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推荐网络例句

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特,那个是爱朗。

Articulate the aims, objectives and key aspects of a strategic business plan.

明确的宗旨,目标和重点战略业务计划。