皮膜

Under light microscope, on H-E stain section, four types cells can be distinguish: cells with weak basophilic fibrillar elements; cells with acidophilic granular substance; cells with strong basophilic fibrillar elements and ciliated cells. In the basal lamina region under gland epithelium, there are a few connective tissue; Surface view of the hypobranchial gland could be see by scanning electron microscope, there are cilia and different kinds of secretions distributed. Ultrastructure of the hypobranchial gland could be understand by transmission electron microscope, supporting cells, sensory cells and seven types gland cell were observed to form the glandular epithelium; cells with much rough endoplasmic reticulum,smooth muscle fiber and nerve endings were found beneath glandular epithelium, among basal lamina region.

光镜下H-E染色切片中腺上皮区仅可以区分出四种类型的细胞：弱嗜碱性纤维样细胞、强嗜碱性纤维样细胞、嗜酸性颗粒分泌细胞和纤毛细胞等；腺上皮下的基底膜区有少量结缔组织存在；扫描电镜下可以观察到鳃下腺表面的纤毛及腺细胞的分泌物等情况；透射电镜下观察到腺上皮中有支持细胞、感觉细胞和7种类型的腺细胞；近基底膜区观察到富含粗面内质网的细胞；基底膜间为薄层疏松结缔组织，内含肌细胞及神经末梢等结构。

Decidual tissue is a very complex and highly concordant tiny environment between mother and embryo during pregnancy, which is constituted of the variety cells such as extravi11ous trophoblast,endometrium cells, bone marrow cells, decidua l microphage,T cells and a few B cells and so on.

正常蜕膜组织是母-胎界面间一个非常复杂而又高度协调的微环境，它由多种细胞如侵袭至蜕膜的绒毛外滋养细胞(extravi11ous trophoblast，EVT)、子宫内膜源性细胞(如上皮细胞和血管内皮细胞)、骨髓源性细胞(如大颗粒淋巴细胞、蜕膜巨噬细胞、T细胞和少许B细胞)构成，这些细胞以及由这些细胞、母体和胎儿所分泌到蜕膜的细胞因子共同形成了母-胎间信息交流的平台，并对胚胎滋养细胞"有控性"侵袭、子宫内膜的免疫容受性、蜕膜化和胎盘的形成进行精细的调控。

Samples of the composites were taken for histological examination at day 7 and day 14 since OKCs were seeded. Results Multi-layer epithelia without hemidesmosome and basal membrane were observed in composites of PGA showing ill-defined cell layers. OKCs could adhere and proliferate onto collagen membrane forming multi-layer epithelia, with no mature hemidesmosome and basal membrane. That of Alloderm showed normal epithelial structures, with hemidesmosome, basal membrane and cell pseudopod in lamina propria.

结果 以PGA为支架构建的组织工程化口腔黏膜，含类似上皮层和固有层，但细胞层次不清；胶原膜上可见口腔黏膜上皮细胞黏附生长，并形成复层上皮样组织，但无成熟的桥粒及基底膜样结构；以Alloderm作为支架材料的构建物，出现正常上皮形态，含半桥粒及细胞指状突起等超微结构。

Results: IL-8 protein was located in the epithelium of viii. The expression of IL-8 was significantly increased in RSA group than that in the control group. Positive IL-8 cells was shown in decidua of RSA group, and its IL-8 level was higher than that in the control group. By hematoxylin-eosin staining. trophoblastic layer was found to get thinner, cells of trophoblastic layer denatured or necrotized, and turned acidophily, and the fibration of villous axis increased in RSA; decidual cells lost connection, a part of decidual cells appeared cytoclasis and turned more acidophilic, and nuclei disappeared in RSA.

结果：在绒毛组织中，IL-8蛋白定位于绒毛上皮细胞的细胞质内，且病例组的表达明显高于对照组；在蜕膜组织中；病例组蜕膜细胞的胞质内可见IL-8蛋白表达，且高于对照组；H-E染色可见病例组绒毛组织的滋养层变薄，细胞变性甚至坏死、嗜酸性增强，绒毛中轴纤维化程度增强；蜕膜组织中蜕膜细胞失去细胞间连接，部分蜕膜细胞解体、核消失。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现：小线角木蠹蛾性信息素分泌腺位于腹部末端8～9节之间，是一个由节间膜特化而成的上皮结构，为一可外翻的腹褶，腺体表面分布着饱满的锥形体，羽化后2天未交尾的雌蛾腺体细胞呈单层排列，腹面中央由密集的柱形上皮细胞组成，细胞排列向两侧延伸至背部，其形状由柱形逐渐变为扁平形，细胞核为椭圆形，细胞与细胞间有明显的胞连接，细胞基底膜基褶较多，质膜上分布着微绒毛，并与内表皮连接，内表皮上有多层几丁质，外角质层染色较深，细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

We evaluated 350 tumors: 136 ES, including 64 conventional ES, 64 proximal-type ES, and 8 with hybrid features of conventional and proximal-type ES; 54 metastatic carcinomas (22 from lung, 6 breast, 6 stomach, 5 colorectum, 5 kidney, 5 prostate, 5 pancreas); 12 metastatic testicular embryonal carcinomas; 20 metastatic melanomas; 20 epithelioid mesotheliomas; 20 epithelioid angiosarcomas; 10 epithelioid hemangioendotheliomas; 24 epithelioid malignant peripheral nerve sheath tumors; 22 myoepithelial carcinomas of soft tissue; 7 anaplastic large cell lymphomas; 5 histiocytic sarcomas; and 10 control MRT of infancy (4 brain, 3 liver, 2 soft tissue, 1 kidney).

我们总共研究了350例肿瘤：136例ES，其中包括64例寻常型ES、64例近端型ES和8例混有前两型特征的混合型ES；54例转移癌（22例来自肺、6例来自乳腺、6例来自胃、5例来自结直肠、5例来着肾、5例来着前列腺、5例来着胰腺）；12例转移性睾丸胚胎性癌；20例转移性黑色素瘤；20例上皮样间皮瘤；20例上皮样血管肉瘤；10例上皮样血管内皮瘤；24例上皮样恶性外周神经鞘膜瘤；22例软组织的肌上皮癌；7例间变性大细胞淋巴瘤；5例组织细胞肉瘤；10例作为对照的婴幼儿MRT（4例位于脑、3例位于肝、2例位于软组织、1例位于肾）。

MTT assay FAK signaling pathway inhibitor genistein on human corneal epithelial cell cytotoxicity;RT-PCR detection of human corneal epithelial cells adhesion to fungus at different times,extracellular matrix protein including laminin,fibronectin,FN glass,Ⅳcollagen,transmembrane protein integrinαⅤ,integrinβ1(ITGβ1),as well as the FAK signaling pathway FAK1,FAK2 and Paxillin gene expression;Western blot detection of the signal transduction pathway adhesion-associated protein ITGβ1,FAK and PAX expression and the inhibition of genistein. Immunocytochemical method was used to observe the LN,FN and FAK expression in human corneal epithelial cells during interaction with the fungues;Laser scanning confocal microscope had a cell positioning on FN,FAK and PAX,observed the changing of the human corneal epithelial cytoskeleton after stimulated by fungues;Quantitatived by flow cytometry to detect of human corneal epithelial cells with PAX at ITGβ1 fungal expression after adhesion;Optical microscopy quantitied the fungues and human corneal epithelial cell adhesion and recorded to determination the integral optical density afrer adhesion;Scanning and transmitted electron microscope observed the changing of cell ultrastructure after fungues and human corneal epithelial cell adhesion.

第一部分真菌激活人角膜上皮细胞FAK信号转导通路的体外实验研究将三种常见致病真菌(白色念珠菌、烟曲霉菌和茄病镰刀菌)分别与人角膜上皮细胞共孵育，MTT法检测FAK信号通路抑制剂染料木黄酮的对人角膜上皮细胞的细胞毒性作用；RT-PCR检测真菌黏附人角膜上皮细胞后不同时间细胞外基质层连蛋白、纤连蛋白、玻连蛋白、Ⅳ型胶原、跨膜蛋白整合素αV、整合素β1(ITGβ1)，以及FAK信号通路中FAK1、FAK2和桩蛋白基因的表达情况；Western blot的方法检测黏附信号转导途径相关蛋白ITGβ1、FAK和PAX的表达，以及染料木黄酮对真菌刺激人角膜上皮细胞FAK信息通路活化的抑制作用；免疫细胞化学方法观察人角膜上皮细胞与真菌相互作用过程中LN、FN和FAK的表达；激光共聚焦显微镜对FN、FAK和PAX进行了细胞定位，并观察真菌刺激后人角膜上皮细胞骨架的变化；流式细胞仪定量检测人角膜上皮细胞ITGβ1与PAX在真菌黏附后表达的改变；光学显微镜观察真菌与人角膜上皮细胞黏附数量，记录并测定了黏附后积分光密度值OD扫描及投射电镜观察了真菌与人角膜上皮细胞黏附后，细胞超微结构的改变。

By setting different kinds of filters of various thickness values before the pinholes of camera and using the spectral sensitivity of the camera,multi-frame X-ray images of wire arrays Z-pinch implosions in different energy ranges were obtained. By analysing the relationship between the intensity profiles of corresponding frames and the spectral sensitivity of the camera,it is allowed to estimate X-ray photon energy range in local areas of images.

通过对皮秒分幅相机和2.3μm厚formvar膜滤片的光谱响应特性分析，以及对同一时刻粘贴2.3μm厚formvar膜滤片和未粘贴2.3μm厚formvar膜滤片两幅图像径向辐射强度波形的对比，确定出获取的X光辐射图像的能谱范围：不加formvar膜滤片的X光辐射图像光子能量大于70eV，加formvar膜滤片的X光辐射图像光子能量大于150eV，同时还确定出图像各部分的光子能量分布。

Results The expression of endogenous LN was found in the basement membrane of epithelium, glands and blood vessels in uterine endometrium of both estrus and early pregnancy. And its was faint that LN present in basement membrane as the implantation occurs, but the LN immunostaining in the epithelial cells of endometrium was stronger. The exogenous LN has no obvious influence on blastocyst attachment and implantation. LN-antibody intra-uterine injection showed significant repressing effect on mouse blastocyst implantation.

结果 动情期及妊娠早期小鼠子宫内膜内源性LN在上皮和腺基膜及血管内皮基膜均有表达，并随着植入的发生基膜的LN表达减弱，而内膜上皮中LN免疫染色逐渐增强；外源性LN对小鼠胚泡粘附与植入子宫内膜不产生明显影响，层粘连蛋白抗体明显抑制小鼠胚泡着床。

Special line: PET bottles to send the wind line, PET bottles transmission line, eight-treasure Li transportation, storage assembly line speed chain, refrigerator-line detection of, stainless steel transmission lines around with CPT, conveyor Store line, groove belt, transportation of finished Line, the size and quality control systems, improve water vat, with tailgate climbing belt, with the assembly line stands, the knife-edge transition belt turn, to roll lines, cell delivery systems, computer assembly lines, power assembly lines, power lines Raceway , To send the wind-line, high-speed sorting lines, transmission lines Raceway, drum line, after the assembly lines overhang, the chemical industry transmission lines, aging transmission lines, Tetra transmission lines, transmission lines plate chain, chain lost keel box line, spiral Chain plate line, spiral belt, a motorcycle transmission lines, motorcycle assembly line, climbing belt, a belt transmission line group, bottles of beer delivery systems, aerodynamic balance suspended, the lines between the pneumatic hoist, automotive transmission lines, light-top Transfer or devices, thermal plastic packet transmission line, stretching line wheel, food delivery lines, transmission lines, transmission lines climbing boxes, fruit cleansing transmission lines, the level of circulating conveyor chain, no power lines Raceway, no power telescopic Raceway lines, lines between the hoist, the letter-box sorting systems, conveyor suspension, tobacco conveyor, the parcel sorting conveyor systems, packet transmission lines, packet transmission line combinations, straight plate section, gravity-shelf access device , Heavy-duty power lines Raceway, heavy-duty conveyor chain, chain of beads to lose face envelope, turning plate, vehicle loading and unloading conveyor, the assembly line, assembly line, automatic transmission storage systems, feeding

特殊流水线：PET瓶风送线，PET瓶输送线，八宝粥输送骊，倍速链储装配线，冰箱检测线，不锈钢输送线，彩管绕带机，仓储输送线，槽型皮带机，成品输送线，尺寸与质量检测系统，大桶水提升设备，带挡板爬坡皮带机，带灯架装配线，刀口过渡转弯皮带机，地滚线，电池输送系统，电脑装配线，动力装配线，动力滚道线，风送线，高速分拣线，滚道线输送系统，滚筒线，后悬装配线，化工行业输送线，老化输送线，利乐包输送线，链板输送线，龙骨链输箱线，螺旋链板线，螺旋皮带机，摩托车输送线，摩托车装配线，爬坡皮带机，皮带输送线组，啤酒瓶输送系统，气动平衡吊，气动线间提升机，汽车输送线，轻型顶升移载装置，热塑膜包输送线，伸缩滚轮线，食品输送线，输送线，输箱爬坡线，水果输送清洗线，水平循环链式输送机，无动力滚道线，无动力伸缩滚道线，线间提升机，信盒分拣系统，悬挂输送机，烟草输送机，邮包输送分拣系统，邮包输送线，邮包组合输送线，直段板式机，重力式货架存取装置，重型动力滚道线，重型链式输送机，珠面链输包线，转弯板式机，装卸车输送机，装配流水线，装配线，自动仓库输送系统，上料机

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。