皮肤试验剂量

Results Acute oral LD50 were 278 mg/kg and 56.2 mg/kg in rats and mice respectively, while dermal LD50＞2 000 mg/kg and inhalative LC50＞3 000 mg/m3. Skin and eye irritancy were estimated to be negative and mild respectively. Cumulative coefficient was 2.3 and allergic rate was 0%. A negative result was observed in Ames assay while increased frequencies of bone marrow micronuclei and spermatocyte chromosome aberrations were noted in the high dose (11.24 mg/kg) group.

结果 大小鼠急性LD50分别为278 mg/kg和56.2 mg/kg；大鼠经皮LD50＞2 000 mg/kg；急性吸入LC50＞3 000 mg/m3；无皮肤刺激性，轻度眼刺激性；蓄积系数2.3；致敏率为0；Ames试验结果阴性；微核和染色体在高剂量组(11.24 mg/kg)与阴性对照组相比有高度显著性差异(P＜0.01)；大鼠90天亚慢性经口的最大无作用剂量为2.825 mg/kg。

Methods: According to the refe-rence of new traditional Chinese medicine study, maximal dosage of drug was given to rabbits to do the acute toxicological and irritative test on skin. The guinea pig was used to do the skin hypersensitive test with excipient as negative control and 1% 2,4-dinitrochlorobenzene as the positive control.

参照中药新药研究指导原则，采用最大给药剂量法对大白兔进行皮肤用药急性毒性和刺激性试验；以赋形剂为阴性对照，1%的2，4-二硝基氯苯丙酮溶液为阳性对照，对豚鼠进行皮肤过敏性试验。

Methods: Distribute NIH rat, or cavia porcellus 40 to four 4 groups:Fast-recovery Injury Liquid Membrane、xiaoyanShan、WanHuaYiu、con trol group, They were experimented with antiinflamation, analgesia on; Distribute randomly cavia porcellus 36 into 3 groups:Fast-recovery Injury Liquid Membrane、Model group、Control group, they were experimented with treatment of acute soft tissue injury on; cavia porcellus 4, they were experimented with skin sensitization self-controlly; Distribute rabbit 20 to 5 groups, them were experimented with LD50 on.

将NIH小鼠或豚鼠随机分为4组，每组分为10只：伤速康涂膜液组、消炎散组、跌打万花油组、基质组分别进行抗炎、止痛实验；将豚鼠随机分成3组：伤速康涂膜液组、模型组、空白组进行伤速康涂膜液对软组织损伤模型的实验；将豚鼠4只，予以自身对照，进行皮肤过敏试验；将家兔20只，随机分为5组：破损皮肤高剂量组、破损皮肤低剂量组、完整皮肤高剂量组、完整皮肤低剂量组和对照组进行急性毒理学试验。

The result fights allergic experiment to show, dispel the wind stops land of can different level restrains urticant Ding allergy of akin and passive skin mixes the bandicoot that antiserum causes 2, the sex of small rat late hair of chloric benzene be caused by exceeds 2 nitro- of 4- quick the thymus index that reaction; still can increase small rat when dosage greatly and lienal index; stop urticant experiment shows, dispel the wind stops urticant Ding can reduce the time of fit of small rat itching with dextrose lead anhydride and itching duration apparently, can increase Guinea pig to be able to bear or endure those who suffer phosphoric acid histamine send urticant threshold.

结果抗过敏试验显示，祛风止痒酊能不同程度地抑制抗血清诱发的大鼠同种被动皮肤过敏和2，4-二硝基氯苯所致的小鼠迟发性超敏反应；大剂量时还可增加小鼠的胸腺指数及脾指数；止痒试验显示，祛风止痒酊能明显降低右旋糖酐诱导的小鼠搔痒发作次数及搔痒持续时间，并能增加豚鼠耐受磷酸组胺的致痒阈。

Individuals who have a positive history but a negative skin test tolerate penicillin-class antibiotics as well as they tolerate other antibiotics, according to AAAAI literature. Although the skin test is as safe as skin testing for other allergens, a "very low rate of resensitization" will occur after skin testing and reuse of penicillin. The skin test exposes the patient to an amount of penicillin that consists of 1/5,000th of that in a typical prescription.

根据AAAAI的文献指出，有盘尼西林过敏的病历却在盘尼西林测试中出现阴性反应者，对其他抗生素有著一样的耐受特质；虽然盘尼西林皮肤测试和其他的过敏原皮肤试验具有一样的安全性，盘尼西林在经过皮肤试验及使用过后，再度产生敏感的比率却非常的低；皮肤测试中，患中会暴露在正常盘尼西林处方剂量的5000分之一。

The cells were shammed or irradiated with different dosages of UVB radiation and treated with the above TCM monomers. The damage of fibroblasts was observed with inverted phase contrast microscope.Cellular proliferation activity was detected by MTT method.The secretory volume of IL-6 and TNF-α was measured by ELISE. Results The damage degree of the cells irradiated was dependent with the irradiated doses and the proliferation activity reduced by 28%-44%.

采用30mJ/cm^2、60mJ/cm^2、90mJ/cm^2剂量UVB照射体外培养的原代人皮肤成纤维细胞，以中药单体EGCG和黄芩苷进行干预处理，倒置相差显微镜观察细胞受损程度，以甲唑盐比色试验检测细胞增殖活性，酶联免疫吸附试验检测上清液TNF-α、IL-6分泌量。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。