皮肤移植法

Objective To explore a good method for the treatment of earlobe defect .

目的：探讨采用不同方式的皮瓣法尤其是皮肤扩张术+自体肋软骨移植再造法对不同程度的耳垂缺损畸形患者进行手术整复。

10% chloral hydrate anesthesia and glass cannula enswathement are availability for mouse tail skin transplatation. The failure rate of this method is low. It is suitable for students experiment.

10%水合氯醛麻醉结合玻璃套管法包扎进行小鼠尾部皮肤移植是一种有效、简便、经济的小鼠尾部皮肤移植手术实验方法；此方法技术失败率低，适合学生实验课采用。

The recipients were divided into four groups which received 0.7%, 1%, 1.5% pentobarbital sodium and 10% chloral hydrate intraperitoneum injection respectively And the other recipients were divided into two groups which received glass cannula and adhesive bandages enswathement method, respectively.

分别用0.7%、1%、1.5%戊巴比妥钠和10%水合氯醛对小鼠进行麻醉试验，选出合适的麻醉方法；再进行小鼠尾部皮肤移植，对比玻璃套管法和创可贴法2种包扎方法的效果，以选出合适的手术方法。

The skin autografting which is widely used at present has cured numerous patients, but it still confront many cruxes, such as lack of donor sites, increasing in wound area caused by donor sites, contracture of grafted thin split skin and shortage of skin appendages, etc.

目前在烧伤的临床治疗中所广泛采用的自体断层皮片移植法虽然成功救治了众多患者，但也面临着严重烧伤时自体皮源不足、增加创面面积、移植后皮肤挛缩及缺乏皮肤附件等难题。

When electric fusion method was used for nuclear transfer, the fusion rate (46. 0%), cleavage rate (53. 9%) and blastocyte development rate (10.9%) of adult ear fibroblasts were significantly lower than that of fetal fibroblasts (64. 5%, 70.1%, 21. 6% respectively), fetal skin cells (71. 5%, 70.8%, 22. 1% respectively) and ovary granulosa cells (88. 2%, 79. 1%, 25. 5% respectively). There was no significant difference among other donor cells in the cleavage and blastocyst development rate of resconstituted embryos.

当用电融合法进行核移植时，成体耳部成纤维细胞的融合率（46.0％），卵裂率（53.9％）和囊胚发育率（10.9％）均显著低于胎儿成纤维细胞（64.5％，70.1％和21.6％），胎儿皮肤细胞（71.5％，70.8％和22.1％），以及卵巢颗粒细胞（88.2％，79.1％和25.5％）；另外三种细胞间的卵裂率，囊胚发育率无显著差异，但卵巢颗粒细胞的融合率显著高于胎儿成纤维细胞和胎儿皮肤细胞（88.2％vs 64.4％，71.5％，P＜0.05）。

For a long time, the traditional methods in genetic monitoring of inbred strains include skin grifting test, coat color test and biochemical marker analysis, etc.

长期以来，应用于近交系小鼠遗传质量检测的方法主要有：皮肤移植法、毛色基因检测及生化位点检测技术等。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。