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Methods: Eighty healthy adult SD rats were respectively implanted with MSCs/β-TCP complex in left thigh and β-TCP in right thigh. According to different treating methods, the materials were enveloped with, or transfixed into the supereme genual periosteal flap, or enveloped with superficial epigastric deep fascia flap, or embedded into subcutaneous thigh.

80只成年SD大鼠,根据植入材料不同分2组,即左侧的复合MSCs的β-TCP生物陶瓷和右侧的单纯β-TCP生物陶瓷,再按不同的处理因素分为带膝最上血管骨膜支的骨膜瓣包裹组和贯穿组,带腹壁浅血管的深筋膜包裹组和直接股部皮下埋放组。

Needle taking place is commonly used the rescue that changes cure and danger to weigh a patient at patient of children, tumour, in infusion, because secure not firm outside can bringing about syringe needle slippery haemorrhage to be in charge of, cause local strut, aching, serious person can send infiltration of the certain pharmaceuticals that change cure to cause subcutaneous tissue hypodermically necrotic or the liquid cannot be defeated in time note and illness of incur loss through delay, new puncture increases the patient's anguish not only, also increased economic burden to also can be affected at the same time to the patient nurse working quality, the course relapses clinical experiment, my division adopts vein to accept the annular and fixed method of buy needle, obtained better result, introduce as follows now. 1 method is taken fixed stick (needle taking place is deployed) together, the disinfection that wide 10cm controls wraps cloth, two paragraphs of end are seamed on after sticking glue to buckle vein to leave buy needle puncture to succeed, convention is secured with adhesive plaster, next the ground degree of tightness will take stalk of handle of buy pinhead, needle, needle to be in firmly aptly fixed and fixed stick , next annular in blood-vessel twine stick on stick buckle. 2 experience apply this method to secure vein to left buy needle method to reduce the patient's anguish, reduced cost, provided mobile space for the patient, also avoid heparin cap prolapse, still facilitate observation puncture is nodded, do not affect hematic use, gauze pad can alleviate heparin cap is oppressive and unwell, reduce infusion pollution.

概要: 留置针常用于小儿、肿瘤患者化疗及危重患者的抢救,在输液中,由于固定不牢可导致针头滑出血管外,引起局部肿胀,疼痛,严重者可致某些化疗药物渗入皮下造成皮下组织坏死或液体不能及时输注而延误病情,重新穿刺不仅增加病人的痛苦,也给病人增加了经济负担同时也会影响护理工作质量,经过反复临床实验,我科采取静脉留置针的环形固定方法,取得了较好的效果,现介绍如下。1方法取固定贴一块,宽10cm左右的消毒包布,两段端缝上粘胶扣静脉留置针穿刺成功后,常规用胶布固定,然后将根据松紧适宜将留置针头、针柄、针梗处牢牢固定固定贴,然后在血管中环形缠绕粘上粘扣。2心得应用此方法固定静脉留置针方法减少了患者的痛苦,降低了费用,为患者提供了活动的空间,也避免肝素帽脱垂,还便于观察穿刺点,不影响血运,纱布衬垫可缓解肝素帽压迫不适,减少输液污染。

Percentage of dressing, eviscerated carcass decreased significantly as dietaryω6/ω3 was reduced; percentage of abdominal fat, subcutaneous fat and intermuscular fat were higher in birds fed lower ratio diets. Drip loss in both breast and thigh muscle increased significantly in birds fedω6/ω3 ratio from 18 to 2.6. Except b* value in breast muscle decreased as birds fed dietary ratio from 5 to 2.6, the tendency for breast and thigh muscle were almost similar; a* values progressively increased as the diets contained increasingω3 content, changes in b* were also significant but the pattern was almost the reverse for changes in a*. Decreasing the dietaryω6/ω3 clearly increased the contents of ALA, EPA and DPA, but not for DHA.

结果表明:①饲喂10:1的日粮显著增加6周龄AA鸡的体重和日采食量;②随着日粮ω6/ω3比值的降低,屠宰率和净膛率显著下降;低ω6/ω3比值的日粮增加腹脂率、皮下脂肪和肌间脂肪;③日粮ω6/ω3比值从18下降到2.6时,胸、腿肌滴水损失显著下降;除日粮比值从5降到2.6时胸肌b*值下降外,胸、腿肌变化规律相似,a*值和b*值变化趋势相反:a*值下降,b*值上升;④日粮ω6/ω3的降低,ω3 PUFA中ALA、EPA和DPA显著增加;⑤肝脏中ALA含量低于胸肌,而其长链衍生物C20:3、EPA和DHA的含量则是肝脏中含量较高。

The investigation in surface lineal defect of rod are carried on by means of metallograph,grinding test as well as deformation test during roughing roll.

通过对试样进行金相分析,对铸坯表面修磨试验结果分析及对连铸坯在高线粗轧第1道和第2道轧制的受力和变形的分析,发现盘条表面的一种特殊的线状缺陷形成的原因是连铸坯存在边内裂和皮下气泡,粗轧机组(特别是第1和第2架)孔型设计不合理,也促进这种线状缺陷的形成。

Special staining methods, such as Masson and the Van Gieson staining were used to study the distribution of collogen fibers and elastic fibers. ResultsBy HE staining, the subepithelial connective tissues and vessels in the pterygium were more prominent than normal conjunctival tissues. An amorphous subepithelial superficial hyalinized zone and coarse eosinophilic granular materials were observed in the pterygia, but they were not found in normal conjunctival specimens. Coarse fibers were visible only in the deeper subepithelial connective tissues of pterygial samples. With Masson′s staining, the dense staining of collagen fibers was also more prominent in the pterygium than in the subepithelial connective tissues of normal conjunctiva. Abnormal collagen fibers were visible in the deeper sub-epithelial connective tissues of pterygial samples. With Van Gieson staining, abnormal collagen fibers were visible in the deeper subepithelial connective tissues. Dark coarse elastic fibers were found in the abnormal fibers only in the subepithelial deep connective tissues of pinguecula in the pterygia but not in the conjunctiva. With immunohistochemistry staining, MMP-3 was strong in the pterygial epithelium, moderate in fibroblast and absent from pterygial vascular walls. LN was strongly expressed in the blood vessel wall, moderately in the epithelial basement membrane and absent from the entire stroma.

结果HE染色:翼状胬肉组织上皮下基质中存在结缔组织的增生和血管形成;基质浅层存在一无定形物质透明区及粗糙的颗粒样嗜酸性物质,在翼状胬肉体部深层基质中存在粗糙的纤维组织;正常球结膜组织细胞排列整齐;基质为疏松结缔组织,胶原纤维平行排列,其间可见成纤维细胞,散在少量中性粒细胞、毛细血管;Masson染色:翼状胬肉浅层基质中存在致密的胶原纤维染色,深层基质中的胶原纤维存在变性样改变;VG染色:翼状胬肉组织深层基质中存在大量变性的胶原纤维,其间夹杂黑色的弹性纤维;免疫组化染色法:MMP-3在翼状胬肉上皮细胞中呈强表达,成纤维细胞中呈中等强度表达,血管内皮细胞中未见表达;LN在血管壁中呈强表达,在上皮细胞基底膜中呈中等强度表达,在整个基质中未见明显表达;col Ⅲ在整个翼状胬肉基质中呈强表达。

By comparing the body weight, immune organs index and response of lymphocytes to ConA, to analyze the effect of QKL on immunological system of normal mouse. Delayed type hypersensitivity mouse model was established by stimulation with dinitrofluorobenzene. By comparing spleen index and swelling rate of pathogenetic ear, to illuminate the immunoregulation of QKL in vivo. Polymicrobial sepsis mouse model was induced by cecal ligation and puncture. The cumulative survival rate was observed in QKL and NS groups. Results: QKL had no influence on body weight and spleen index of normal mouse, but it could significantly decrease thymus index and inhibit the immune response of lymphocytes to ConA.

连续皮下注射QKL 5 d,通过检测小鼠体重、免疫器官指数和淋巴细胞对ConA刺激的反应,分析QKL对正常小鼠免疫功能的影响;采用二硝基氟苯诱发小鼠耳朵肿胀,发生接触性皮炎,建立DTH模型,通过比较QKL与NS对照对小鼠脾脏指数和发病耳肿胀抑制率评价QKL体内对小鼠的免疫调节功能;采用盲肠结扎穿孔术后引起的多细菌性腹膜炎,建立小鼠脓毒症模型,通过观察QKL对小鼠存活状况的影响,评价QKL的免疫调节作用。

Unlike X-rays, the standard radiotherapeutic tool, a proton beam can be tuned in a way that causes it to dump its destructive energy at a particular depth beneath the skin. This means it can destroy a tumour without damaging other tissue.

与标准的放射治疗手段—— X 射线不同,质子射线的强度是可以调节的,待进入皮下特定的深度之后才一次性释放杀伤性能量,这说明它可以在破坏肿瘤的同时不会造成其他组织的损伤。

Results:the incision complications of the 31 cases,include incision infection(12 cases),subincision fluid(11 cases),incision dehiscence(1 case),incision bleeding(1 case),subcutaneous congestion and hematoma(1 case),incisional hernia(2 cases), incision tumor implantation(1 case),and incision hyperplasia scar (2 cases).conclusions:it is important to prevent incision complications of lc from occuring by strict asepsis,reasonable incision selection,careful and standard technical operation,well anaesthetic coordination and resonable postoperotive treatment.

回顾1 650例lc发生切口并发症31例的临床资料。结果:31例中切口感染12例,切口下积液11例,切口裂开1例,切口出血1例,皮下淤血及血肿1例,切口疝2例,切口肿瘤种植1例,增殖性瘢痕2例。结论:严格的无菌技术,恰当的切口选择,细致和规范的技术操作,良好的麻醉配合和合理的术后处理是预防lc切口并发症的重要措施。

In nude mice, large and invasive tumors were found after injection of HuH7 cells subcutanesously. While mice injected with SeLT cells demonstrated highly vacuolated cells-filled tissue masses, severe necrosis, 4 inflammation as well as degenerated blood vessels.

利用皮下注射肝癌细胞株到裸鼠之后,实验发现HuH7 形成较大的肿瘤、有大量的血管形成且有转移的现象,而SeLT 虽然能够形成tissue mass 但其中大部分的细胞都有严重坏死及发炎的现象产生,伴随著新血管也呈现有萎缩的现象。

It was shown that: 1 MMP2 activity reached the highest level during proestrus, and was lowest during diestrus; 2 MM2 acti vity was increased from the first day of pregnancy, peaked on day 4 of pregnancy; but decreased significantly at day 7 of the pregnancy. 3 MMP2 activity was obviously higher on day 1 of pseudopregnancy than on the other days, and its activity decreased gradually, reached the very low level at day 4 of pseudopregnancy and maintained the low level until day 7; 4 MMP2 activity was decreased significantly 24 hours after injection of 17βestradiol ( 01 mg) at metaestrus, while MMP2 activity had no change after progesterone (04 mg) injection.

明胶酶A活性变化如下:1在大鼠的动情前期明胶酶A的活性最高,间情期最低;2在妊娠早期(1~7天)的卵巢中,明胶酶A的活性在妊娠第4天达到最高,第1~3天及第5、6天其活性也较高,但第7天则明显下降;3在假孕(1~7天)大鼠的卵巢中,明胶酶A的活性在假孕第1天最高,第2、3天略下降,从第4天到第7天,其活性都处于较低水平;4动情后期大鼠皮下注射雌二醇(0.1mg/只)24小时后,明胶酶A活性明显上升。

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