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Material and MethodsRA Rabbit Model group: 15 early RA rabbits of the same weight and variety Control group:10 normal rabbits of the same weight and varietyMethods of making RA rabbit model:elect 15 normal rabbits of the same weight and variety, dissolve Ovalbumin in 0.9% sodium chloride solution, to make the solution of 20mg/ml concengtration, blend the same quantity of complete Freund′s Adjuvant equably ,inject the mixed solution into endermic tissue of the rabbit′s scapular section, making the rabbits allergic, inject 1 ml of the mixed solution to one rabbit every time, inject 1 ml of the mixed solution in 5 different places of the rabbit′s scapular section, inject the rabbit one time every week,inject 3 weeks continuously,it turned out to be that the rabbits will be allergic, inject Ovalbumin blent with the 0.9% sodium chloride solution into the knee joint cavity of the rabbits in the fourth week, 5 mg Ovalbumin every knee joint cavity,two knees of every rabbit of the 15 rabbits are both injected,the arthrosis diameter and the exterior temperature of the knees will be added obviously in 24 hours,and they will drop gradually,at the time of the 14th or 21th day, the arthrosis diameter and the exterior temperature of the knees will get to the balance time,the incidence rate of RA is 100%.after the RA model succeeds,it is the early time of RA from the first week to the fourth week,after the fourth week,it is the late time of RA, the cartilage of the femoral condyle and the subchondral bone cortices will be changed unrecoverily.

资料与方法RA模型组:早期RA家兔模型15只,品种及体重接近。对照组:正常家兔10只,品种及体重接近。RA家兔模型制作方法:选取15只成年家兔,体重、品种接近,将卵蛋白溶解于生理盐水,配成浓度为20mg/ml的溶液,与等量完全弗氏佐剂混匀,注入家兔肩胛区皮下致敏,每只家兔每次注射1ml,于肩胛区5个不同区域注射,每周一次,连续注射3周而致敏,第4周向膝关节腔注射卵蛋白生理盐水溶液,每只关节腔注射5mg卵蛋白,15只家兔膝关节全部注射,24h内此关节直径和表面温度大幅度上升,以后缓慢下降,至14~21d达到平台期,发病率达100%。造模成功后第1~4周为早期改变,第4周以后出现不可逆的关节软骨及骨破坏。采用高频超声对RA模型组与对照组的膝关节髌上囊液体厚度、滑膜及股骨髁软骨厚度及软骨下骨皮质的回声情况进行对比观察。结果RA组模型组膝关节髌上囊积液及滑膜的厚度明显厚于对照组,其股骨髁软骨的厚度与对照组相比无明显差异,其软骨下骨皮质与对照组相比无明显改变。

Careful attention should be paid to every steps of enterostomy operation in order to prevent the complications of stoma. These include the incision of skin, hypodermis, fascia and peritoneum, fixation of bowel around the stoma aperture, the size and shape of stoma, and the supportive tube in loop ostomy.

要求术者用心做好造口手术的每个细节,包括造口皮肤切口、皮下、筋膜、腹膜等的切开和造口肠管之间的缝合固定,造口的大小和形状,以及襻式造口所用的支撑管,以达到预防造口并发症的目的。

In this report, immunohistochemistry and in situ hybridization techniques were perfected to examine the expression of bFGF, fibroblast growth factor receptor-I and bFGFmRNA of cultured HEP-2 laryngo-tumor cell and 803 gastro-tumor cell and of the solid tumor tissue formed by implanting the cultured cells into subcutis of nude mice, intending to elucidate the correlation between the expression level and the tumor formation.

本研究建立了免疫组化和原位杂交的检测方法,通过对体外培养的HEP-2喉癌细胞和803胃癌细胞和这两株细胞在裸鼠皮下形成的实体瘤组织中bFGF,纤维细胞生长因子受体-1(fibroblast growth factor receptor-1,FGFR-1)和bFGFmRNA的检测试图揭示三者在体外和体内表达以及与肿瘤形成的关系,从而为这方面的研究提供更多的材料。

Result: 1. The method of arc-needle injection with 131I-iodinated oil step-by-step sector-shapely improved obviously the drug distribution, comparing with routine linearity injection. 2. SPECT image reconstruction detect 131I-iodinated oil three-dimensional distribution quantitatively. 131I-iodinated oil metabolism equation may obtained by continuous measurements, in order to calculate absorption dose of p particle at target. 3. ZT glue lengthens obviously 131I-iodinated oil retention in mouse local subcutis and rabbit liver which can rise local radiation effect, decrease injury at normal tissue.

结果:1、弧形针分段扇形注射~(131)I-碘化油,比常规使用的线性注射明显改善药物弥散均匀度。2、SPECT断层扫描定量检测~(131)I-碘化油的三维分布,连续测量建立~(131)I-碘化油的代谢方程,并计算~(131)I对靶区的β辐射贡献的吸收剂量。3、ZT胶明显延长~(131)I-碘化油在大鼠皮下、兔肝脏内的滞留。

I-iodinated oil metabolism equation may obtained by continuous measurements, in order to calculate absorption dose of β particle at target. 3. ZT glue lengthens obviously 〓I-iodinated oil retention in mouse local subcutis and rabbit liver which can rise local radiation effect, decrease injury at normal tissue.

结果:1、弧形针分段扇形注射〓I-碘化油,比常规使用的线性注射明显改善药物弥散均匀度。2、SPECT断层扫描定量检测〓I-碘化油的三维分布,连续测量建立〓I-碘化油的代谢方程,并计算〓I对靶区的β辐射贡献的吸收剂量。3、ZT胶明显延长〓I-碘化油在大鼠皮下、兔肝脏内的滞留。

In Rh2+DDP group 1000uM cisplatine and 100uM gensenoside Rh2 were injected to nude mice by abdomen. Growth status of tumors were observed. Diameter of tumors were measured. After 60 days,〓Tc-MIBI were injected from vein of nude mice tail to perform SPECT exam and calculate the value R of the tumors. Later the nude mice were killed.

将A549DDP细胞和A549细胞分别种植于裸鼠皮下,种植A549细胞的裸鼠为A549组,种植A549DDP细胞的裸鼠分对照组、DDP组和Rh2+DDP组,每组4只裸鼠。A549组和对照组的裸鼠腹腔注射生理盐水,DDP组裸鼠腹腔注射低效浓度的顺铂;Rh2+DDP组裸鼠腹腔注射低效浓度的顺铂及无毒浓度的Rh2。

Methods The nude mice tumors were formed by injection of human ovarian cancer cell line AO and AO/HSV1-tkc (AO cells carried with HSV1-tk gene which was constructed in China) subcutaneously, and then were transplanted to the omentum of nude mice. The filter-passing culture fluid of VPC/HSV1-tkc was injected daily into the peritoneum of the nude mice in the group of in vivo. Finally, all of the three groups of nude mice accepted the treatment of GCV.

先将人卵巢癌细胞AO及携有HSV1-tk基因的AO细胞(AO/HSV1-tkc)注射于裸鼠皮下,形成肿瘤后再移植于裸鼠网膜,并以病毒生产细胞(VPC/HSV1-tkc)的培养液对AO网膜移植瘤进行体内转染,然后以GCV对体外AO/HSV1-tkc转移的肿瘤及经体内转染的AO肿瘤进行治疗。

In a placebo-controlled double-blind study in 10 healthy volunteers standardized subcutaneous haematomas were induced in both thighs by injection of autologous blood with an addition of 125l-fibrinogen (Larsson et al.).

在一安慰剂对照的双盲研究中,将加有1251标记的纤维蛋白原的自体血液注射入10位健康志愿者的双腿部,造成标准皮下血肿。

The sensitive level is different between different day-age Jimpy mice. To induce the BPH model with the 60-70 day-age Jimpy mice, should take the hypodermic Adm-way or add dosage in intragastrica Adm-way.

不同日龄的小鼠对丙酸睾丸酮的敏感程度不同,对60~70日龄的小鼠前列腺增生造模宜采用皮下注射的给药方式或加大灌胃的剂量。

We'll make some of these Eastern orange- phosphate-and-massage-cream professors that think they're the only lozenges in the market look like hypodermic tablets.

那些东部磷酸橙汁和按摩霜的教授们,他们中的一些人认为他们的东西是市场上唯一的锭剂,我们要让他们看起来就像是皮下注射片剂。

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