皮下的

The fusion DNA fragments of ag85b-mpb64 and ag85b-mpb64-esat-6 were obtained by PCR andSOE technique. Various DNA vaccines were constructed with the pcDNA3.1: fusion of two genes, and of three genes, bivalent combinations and trivalent combinations(pCA+pCM+pCE6). BALB/c mice were vaccinated with this DNA vaccines.The mice injected withBCG were positive control and the mice injected with pCDNA3.1 and PBS were negative control.The mice were immunized 3 times with 2-wk intervals. The animals in group BCG were only inoculatedsubcutaneously with 1×10~6 CFU BCG at initial vaccination. The serum IgG titers and IgG isotype weredetermined using iELISA coated with M. bovis PPD and rMAE protein expressed and depurated inprokaryotic expression system every week.

同样，利用PCR和SOE技术，获得牛分枝杆菌mpb64-ag85b和mpb64-ag85b-esat-6融合基因，以pCDNA3.1为载体构建了牛分枝杆菌多价组合和多基因融合DNA疫苗：二基因融合(pCDNA3.1-MPB64-Ag85B，简称pCMA)和三基因融合(pCDNA3.1-MPB64-Ag85B-ESAT-6，简称pCMAE)DNA疫苗；二价组合和三价组合(pCA+pCM+pCE6)DNA疫苗，免疫BALB／c小鼠，以牛分枝杆菌BCG免疫组为阳性对照，以pCDNA3.1及PBS免疫组为阴性对照，共免疫3次，每次间隔2周，BCG组仅初免时皮下免疫1次。1免后每周，以原核表达纯化的重组MPB64-Ag85B-ESAT-6蛋白和牛分枝杆菌PPD为包被抗原，以间接ELISA方法检测血清IgG水平及lgG亚类。

The movements maximally affecting subcutaneous cellular tissue to lose fat are rubbing over and squeezing out, whereas effleurage, pettrisage and vibration are the auxiliary ones.

其中最能影响皮下脂肪细胞组织达到减脂的动作是磨擦和挤压，而按抚、揉捏和震动是辅助方法。

Methods From 2002 to 2007,46 patients with facial skin cancer were repaired with different surgical flaps according to the different circumstance,such as the kite flap on a subcutaneous pedic, nasolabial fold flap, orbucularis oculimuscle of flap and maxillocervical fasci ocutaneous flap.

46例面部恶性肿瘤，根据病灶的部位和范围，扩大切除后分别应用&风筝&皮下蒂皮瓣、鼻唇沟皮瓣、眼轮匝肌蒂颞部皮瓣、耳前窄蒂侧颌颈皮瓣等方法修复创面。

At the end of 8 weeks, subcutaneous teratoma was detected in two nude mice, including 3 layers of tissues: sebaceous gland, fat, glandular organ, glandular epithelium and squamous epithelium. At day 4, embryoid body was found and cells were positive for actin, nestin and keratin.

接种8周末，2只小鼠均形成皮下畸胎瘤，其内存在囊括3个胚层的组织结构，包括皮脂腺、脂肪、腺体及腺上皮、鳞状上皮等。

Experts believe that belly fat is more dangerous than other kinds of fatsos fat cells that grown just under the skin.

专家认为腹部脂肪比普通的皮下脂肪要危险得多。

The content of subcuaneous fat was 90.7% and, glycerides?

%和 10 。4 %。肌间脂肪和皮下脂肪的脂肪酸都以C16：0 、C18：1、C18：2 为主要成分。

Methods We meased the abdominl subcutanous fatty thick (30 case ,4 point,every point 9 times) by 5 MHz urltrosonic wave and analysised the reappearance by paired ttest and analysis of variance and the distribution of coefficient of variance.

方法作者利用5MHz超声波，重复测量了30例志愿者的腹部皮下脂肪厚度，并对重复性进行分析。

Objective To investigate inhibitory effect of sorafenib combined with cisplatin against HepG2 hepatocellular carcinoma tumor xenografts.

目的 探讨多靶点酪氨酸激酶抑制剂索拉非尼联合顺铂对人肝癌裸鼠皮下移植瘤生长的抑制作用。

Methods Mouse spontaneous activities under the influence of the koumine injection were recorded with a photoelectric counter, and canine femoral artery pressure was de termined by CYS-0.5 pressure transducer, respiratory curve described with TB-61 1 tension transducer and electrocardiogram recorded with subcutaneous elec -trodes in the extremities after the ...

方法用光电计数仪测定钩吻素子注射液对小鼠自发活动的影响，用CYS-0.5型压力换能器测定犬股动脉血压，用TB-611型张力换能器记录犬呼吸曲线，针型电极刺入四肢皮下测二导联心电图。

In vivo gene therapy of ovarian cancer by adenovirus－mediated thymidine kinase gene transduction and ganciclovir administration.

羟甲基无环鸟苷对携有单纯疱疹病毒胸腺嘧啶核苷激酶基因人卵巢癌裸鼠皮下移植瘤生长的抑制作用。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！