皮下的

Results There is much more subcutaneous fat in umbilical,epigastrium and hypogastrium region(P＜0.01).Liposuction is indicated in the above area when thickness exceeds 25mm,24mm and 12mm respectively.

结果 脐部、上腹部、下腹部皮下脂肪明显高于其它部位P＜0.01，当以上区域皮下脂肪厚度分别超过25mm、24mm、12mm时，应做为脂肪抽吸术的适应证范围。

An intravenous injection of a narcotic.A hypodermic injection, syringe, or needle .

皮下注射器一次皮下注射，皮下注射器，或皮下注射的针

A hypodermic injection, syringe, or needle.

皮下注射器一次皮下注射，皮下注射器，或皮下注射的针

Some serum samples from ducks immunized with attenuated DPV were assayed with the ELISA kit, and the antibodies were detected first on day 6 or day 9 post-immunization in ducks vaccinated subcutaneously, orally or nasally, and still detectable on day 60 PI.

应用该试剂盒对皮下注射、口服和滴鼻免疫DPV弱毒的20日龄樱桃谷鸭血液中抗DPV特异性IgG抗体效价进行了测定，皮下免疫鸭于第6d，口服和滴鼻免疫鸭于第9d可在血清中检测到DPV特异性抗体，且至第60d时依然可检测到高效价抗体。

Slimming Massage CreamDissolve and remove subcutaneous fat through the contact of cream with skin, accelerate topenetrate into subcutaneous tissue through massage, this instantly dissolve fat, remove proud flesh with outstanding effect in removing fat in some part.

哈宾尼斯纤体按摩膏通过膏体与皮肤的接触，消溶皮下脂肪，按摩、加速膏体向皮下渗透，使皮下脂肪得到更快速的溶解，除去身上的赘肉，使皮下脂肪无所盾形，对局部脂肪的调整作用明显。

METHODS: In the experimental group, ADSCs were cultured with the scaffold and induced by CDMP1(50 μg/L) in basic medium for another 2 weeks. In the control group, SD rat cartilage cells were cultured with elementary nutrient liquid with scaffold for another 2 weeks. Twenty nude mice were imbedded the composite of cells-scaffold of the experimental group in their left armpits and the composites of cells-scaffold of the control group in right armpits. Every ten nude mice were killed at 8 and 12 weeks and were examined by safranine O-fast green and toluidine blue staining.

体外培养的脂肪干细胞复合于支架上，加入诱导液(基础培养液+ 50 μg/L软骨形态发生蛋白1)继续培养2周作为实验组；将SD仔鼠软骨细胞复合于支架上，常规培养2周作为对照组。20只裸小鼠，左侧腋窝皮下均植入实验组细胞-支架复合物，右侧腋窝皮下均植入对照组细胞-支架复合物。8， 12周各处死10只，行甲苯胺蓝和番红O-固绿染色。

Results:the rate of sugillation and hematoma that venepuncture in limbs and scalp was less than jugular veins and groin veins,the difference was significant.

结果：四肢静脉穿刺及头皮静脉穿刺发生皮下瘀血和血肿的几率较小，而颈外静脉和腹股沟股静脉穿刺容易发生皮下血肿。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

objective to compare the anti-aging effects of lycium barbarum polysaccharides and tanshinone on the skin of d-galactose-induced mice aging model.methods 60 female mice were randomly divided into 4 groups (n=15):mice in normal control group received subcutaneous injection of normal saline and mice in the other 3 groups received cervicodorsal injection of d-galactose［1000 mg/(kg1·d)］ for 42 d to establish subacute aging model.the experimental groups included agingmodel group,lycium barbarum polysaccharides ［200 mg／group,tanshinone ［1 500 mg／］ group.forty two days later,the dorsal skin samples were collected to determine sod activities、mda and hydroxyproline contents.

目的 比较枸杞多糖与丹参酮对衰老小鼠皮肤的抗衰老作用。方法 60只雌性昆明种小鼠，随机分为4组(n=15)：正常对照组皮下注射生理盐水；其余3组每日颈背部皮下注射 d-半乳糖［1000 mg/］造成小鼠衰老模型，同时各组分别灌胃生理盐水、枸杞多糖［200 mg/］、丹参酮［1 500 mg/］。42 d后，测定小鼠背部皮肤组织匀浆中超氧化物歧化酶活力、过氧化脂质代谢产物丙二醛含量和皮肤羟脯氨酸含量。

The RF electricity of ice adopts radio frequency technique of the RF dual pole. The frequency can make the water element of the cell which at the certain depth shake at a high speed, it can produce the quantity of heat at 45-60℃ through the quick circumvolved friction. Because the collagen fibre is shrinked, the wrinkle can be tighted. Then the light pyrolysis stimulates collagen and elasticity fibre to become more and arrange once again so as to reach the function of dispelling wrinkle and shrinking pore.

冰电除皱系统采用RF双极射频技术，发射的频率可以在皮下的特定深度内使细胞内的水分子产生高速震动，通过这种高速的旋转摩擦从而产生热量45-60℃，胶原纤维收缩，即刻松弛的皮肤、皱纹被拉紧，随后，真皮层中产生的热效应使胶原增生、重新排列、数量增加，同时修复老化受损的胶原，达到即刻紧肤、长效除皱抗衰老的效果。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！