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After cleaning skin with face cream, dorp proper essence on cotton bud with burette, then coating on acne cyst with the cotton swab, and gently press 1 to 2 minuts, making effective ingredients permeate into the skin.

洁面后,用滴管往棉签上滴适量的精华液,用棉签涂于痘痘及痘印处,并轻压1-2分钟,使有效成分渗入皮下,轻微者每天1 - 2次,严重者每天3 - 4次。

Methods: 80 patients with ecchymosis were divided into observation group (40 cases) and control group (40 cases) randomly. Skin of afflicted part was sterilized with iodophor, then washed with normal saline, and pasted comfeel transparent dressing on afflicted part after dry in observation group. Skin of afflicted part was compressed with 50℃, so% magnesium sulfate, 20 minutes each time, twice a day in control group.

将80例皮下出血患者随机分为观察组(40例)和对照组(40例),观察组将患处皮肤先用碘伏消毒穿刺部位再用生理盐水清洗待干后采用康惠尔透明贴贴于患处,根据是否皱折及膜内胶体渗出情况给予更换;对照组采用温度为50℃,50%的硫酸镁热湿敷处理,每次20min,2次/d。

Methods: Experimental rats were injected with KA (10mg/kg, 5mg/ml) subcutaneously to make status epilepticus model. SE latter 10 day, Use subliminal dosages KA to check falling sickness paroxysm sensibility model; SE latter 10 day , Morris water maze was to evaluate cognitive function in the animals. Changing NPY adopt immunocyte chemistry method to check the rat brain inner is expressed.

本研究给Sprague-Dawley大鼠颈部皮下注射惊厥剂量的红藻氨酸(KA,10mg/kg,5mg/ml),诱发癫痫持续状态(status epilepticus,SE), 10天后,用阈下剂量的KA检测癫痫发作敏感性模型;10天后用Morris水迷宫实验检测动物学习记忆功能变化;用免疫组化技术检测动物海马CA1区CA3区NPY表达的变化。

s180 cells were inoculated into the hypoderm of mouse armpit. different concentration of paa was then given to these mice for seven days by way of peritoneal cavity injection.

在小鼠腋部皮下接种s180细胞,随后用不同浓度的paa给各组小鼠作腹腔注射7 d。

An article, such as a paper diaper or hypodermic syringe, that can be disposed of after one use.

用后即丢弃的物品,一次性物品用后即可丢弃的物品,如纸制尿布或皮下注射器

Methods Aging models were made by hypodermic injection of D-galactose. The rats were given intragastrically with the decoction of Polygonum multiflorum Adenophora paniculate and Radix Salviae Miltiorrhizae at the same time.

用D-半乳糖皮下注射,将2月龄大鼠制成衰老模型,用药组在造模的同时给予制首乌、北沙参、紫丹参3味药灌胃,40 d后用电子显微镜观察各组大鼠肝细胞的形态学变化。

The proteins were purified and used to prepare the rabbit antiserum against caprine IFN-γor IL-2,the results showed that the antibody could react with the purified proteins in Western blotting.

分别用ProBond~蛋白纯化试剂盒纯化,用所获得的重组蛋白纯化产物经三次背部皮下多点注射新西兰白兔,制备了兔抗山羊IFN-γ和兔抗山羊IL-2多克隆血清。

Methods The SpaA was purified by electroelution from NaOH-extracted antigen of Erysipelothrix rhusiopathiae strain C43311. The rSpaA-N was expressed in E. coli BL21 as a soluble protein by IPTG inducing,and purified with GST affinity chromatography. Mice of each group were subcutaneously immunized three times with 50μg or 100 μg of native SpaA, rSpaA-N or NaOH-extracted antigen with in complete or incomplete Freund adjuvant at 2-week intervals.

将猪丹毒丝菌C43311株SpaA-N以可溶形式表达在大肠杆菌BL21中,用GST Bind Resin纯化试剂盒纯化rSpaA-N,采用电洗脱法从猪丹毒丝菌C43311株NaOH提取抗原中纯化天然SpaA,将rSpaA-N、天然SpaA和NaOH提取抗原制成亚单位疫苗,同时设GST及生理盐水对照组,间隔2周分3次皮下免疫小鼠,第3次免疫后2周用100LD50猪丹毒丝菌C43065株进行腹腔攻毒,采用间接ELISA方法检测免疫组小鼠血清的抗体动态变化。

The development and metastasis process of orthotopic tumor models was observed directly by fluorescence microscope,and the size of the hypodermal tumor was measured by vernier.

眼内肿瘤生长情况用荧光显微镜直接观察,皮下肿瘤大小用游标卡尺测量;采用免疫组织化学方法对肿瘤内13种基因的表达进行检测。

objective to investigate whether hypoxia preconditioning exist in adipose tissue,and construct the hypoxemic model of adipose tissue.methods after injected different density cocl2 to rat's subcutaneously of groin,use immunohistochemical method detect hif-1α protein,and use rt-pcr detect hif-1α mrna.results after injected different density cocl2,the adipose tissue of rat's groin express different degree of hif-1αprotein and hif-1αmrna.,and reach the peak when the density is 20-40 mg/kg and the time is 3 h.conclusion hypoxia preconditioning exist in adipose tissue,and the effect of preconditioning is most evidence when the density is 20-40 mg/kg and the time is 3 h.

目的 探讨脂肪组织中是否存在缺氧预适应现象,构建cocl2致缺氧的缺氧模型。方法不同浓度的cocl2大鼠腹股沟区皮下层浸润注射,分别用免疫组织化学法和rt-pcr法检测该区脂肪hif-1α蛋白和hif-1α mrna的表达情况。结果用不同浓度的cocl2行化学缺氧后在不同时间点观察有不同程度的hif-1α蛋白和hif-1α mrna表达,其中,在浓度为20~40 mg/kg,时间为3 h时脂肪组织中hif-1α表达达高峰。结论脂肪组织存在缺氧预适应现象;cocl2的浓度选择20~40 mg/kg,时间选择3 h时缺氧预适应效果较佳。

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