皮下用的

The transter and expression of endostatin gene was analyzed by PCR, the effects of inhibition on proliferation of endothelial cell by endostatin in vitro by MTT method, and the effects on the growth of LoVo、 SGC-7901 in vitro was surveyed, and the growth of expressing endostatin LoVo、SGC-7901 cell line_in subcutaneously of nude mice was also measured.

用MTT法检测内皮抑素对内皮细胞体外增殖的抑制作用，并观察其对LoVo细胞和SGC-7901细胞体外生长的影响，观察表达内皮抑素基因的LoVo和SGC-7901细胞在裸鼠皮下种植的生长。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Alleviate pain: Hot pack can not only alleviate arthralgia, but also alleviate obviously lumbago, sciatica and dysmenorrheal by putting hot water bag on the local pain area for 1 to 2 times per day and 20 minutes per time; absorption of subcutaneous congestion can be hastened by warming subcutaneous hematoma caused by sprain and contusion with hot water bag 24 hours after the injury.

缓解疼痛：热敷不光可以缓解关节疼痛，对腰痛、坐骨神经痛、痛经，将热水袋放在局部疼痛处，每次20分钟，每天1-2次，也可明显缓解疼痛；对扭、挫伤引起的皮下血肿，于受伤24小时后，用热水袋热敷，可以促进皮下淤血吸收。

Law of TT of 肕 of take along sth to sb determines the hyperplasia that EVO and PTX odd in order to and couplet use cells of pair of small rat S180 restrains rate; to build S180 to cultivate model of tumour small mouse hypodermically, observation EVO and PTX odd in order to and couplet use hypodermic to small rat tumour grow inhibition.

采用MTT法测定EVO和PTX单用以及联用对小鼠S180细胞的增殖抑制率；建立S180皮下种植瘤小鼠模型，观察EVO和PTX单用以及联用对小鼠皮下瘤的生长抑制功能。

Cent of patient of 51 breast cancer is the method two groups, use medical group of biology albumen rubber 25 routine breast cancer is improved effect a radical cure glue of use biology albumen is achieved in the operation when art, did not use medical group of biology albumen rubber 26 routine breast cancer is improved effect a radical cure biology albumen gum is not used when art, the 1st day drainage after observing art always makes the discharge, hypodermic case that accumulate fluid after quantity, art, in order to decide medical biology albumen glue is improved to breast cancer the precautionary effect that effects a radical cure fluid is accumulated hypodermically after art.

方法51例乳腺癌患者分为两组，采用医用生物蛋白胶组25例行乳腺癌改良根治术时在手术创面使用生物蛋白胶，未用医用生物蛋白胶组26例行乳腺癌改良根治术时不使用生物蛋白胶，观察术后第1天引流量、术后总引流量、皮下积液情况，以确定医用生物蛋白胶对乳腺癌改良根治术后皮下积液的预防效果。

Relieves pain to the lumbago , ischialgia , menorrhalgia (belong to the cold certificate without exception) not only the hot pack being able to relieve arthrosis pain, a hot-water bag is set free getting along in part pain, every time 20 minutes, 1 ~ also may relieve pain obviously 2 time every day,; To twisting , dampening the subcutaneous haematoma bringing about, in being wounded using the hot-water bag hot pack 24 hours the day afer tomorrow ,can boost subcutaneous extravasated blood admitting.

缓解疼痛热敷不仅可以缓解关节疼痛，对腰痛、坐骨神经痛、痛经，将热水袋放在局部疼痛处，每次20分钟，每天1～2次，也可明显缓解疼痛；对扭、挫伤造成的皮下血肿，于受伤24小时后，用热水袋热敷，可以促进皮下淤血吸收，起缓解疼痛的作用。

Sometimes with his hands before touching fontanelle, will be pulsating, but also can be seen beating, which is before the fontanelle caused by subcutaneous vascular pulsatility, if not larger subcutaneous arteries after, do not see beating, this is normal.

有时用手触摸前囟门，会感到悸动，也可看到跳动，这是前囟门皮下血管搏动引起的，如果皮下没有较大的动脉血管经过，就看不到跳动，这是正常的。

According to the limit condition for avoiding the formation of subsurface blowholes.

从避免产生皮下气泡的极限条件出发，定量分析了国外连铸&缎带钢&专利控制低碳低硅成份的理论根据，分析了钢中残铝量的作用以及降低用铝量的可能限度与前提条件。

Methods Mouse spontaneous activities under the influence of the koumine injection were recorded with a photoelectric counter, and canine femoral artery pressure was de termined by CYS-0.5 pressure transducer, respiratory curve described with TB-61 1 tension transducer and electrocardiogram recorded with subcutaneous elec -trodes in the extremities after the ...

方法用光电计数仪测定钩吻素子注射液对小鼠自发活动的影响，用CYS-0.5型压力换能器测定犬股动脉血压，用TB-611型张力换能器记录犬呼吸曲线，针型电极刺入四肢皮下测二导联心电图。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。