皮下注射

The HL60 cells were inoculatedsubcutaneously to nude mice and after the dimeter of tumorreached 5mm,the NIH3T3-IFN-〓 cells were implanted i.p.intothe tumor-bearing mice or DOX was injected i.p.into the tumor-bearing mice.

将HL60细胞皮下接种BALB/C裸鼠体内，至皮下肿瘤长至直径达5mm时，给荷瘤小鼠腹腔内埋植〓细胞或腹腔注射阿霉素，结果发现其皮下肿瘤生长被显著抑制，特别是当NIH 3T3-IFN-〓细胞与阿霉素联合应用时，肿瘤生长抑制作用最明显。

Material and MethodsRA Rabbit Model group: 15 early RA rabbits of the same weight and variety Control group:10 normal rabbits of the same weight and varietyMethods of making RA rabbit model:elect 15 normal rabbits of the same weight and variety, dissolve Ovalbumin in 0.9% sodium chloride solution, to make the solution of 20mg/ml concengtration, blend the same quantity of complete Freund′s Adjuvant equably ,inject the mixed solution into endermic tissue of the rabbit′s scapular section, making the rabbits allergic, inject 1 ml of the mixed solution to one rabbit every time, inject 1 ml of the mixed solution in 5 different places of the rabbit′s scapular section, inject the rabbit one time every week,inject 3 weeks continuously,it turned out to be that the rabbits will be allergic, inject Ovalbumin blent with the 0.9% sodium chloride solution into the knee joint cavity of the rabbits in the fourth week, 5 mg Ovalbumin every knee joint cavity,two knees of every rabbit of the 15 rabbits are both injected,the arthrosis diameter and the exterior temperature of the knees will be added obviously in 24 hours,and they will drop gradually,at the time of the 14th or 21th day, the arthrosis diameter and the exterior temperature of the knees will get to the balance time,the incidence rate of RA is 100%.after the RA model succeeds,it is the early time of RA from the first week to the fourth week,after the fourth week,it is the late time of RA, the cartilage of the femoral condyle and the subchondral bone cortices will be changed unrecoverily.

资料与方法RA模型组：早期RA家兔模型15只，品种及体重接近。对照组：正常家兔10只，品种及体重接近。RA家兔模型制作方法：选取15只成年家兔，体重、品种接近，将卵蛋白溶解于生理盐水，配成浓度为20mg/ml的溶液，与等量完全弗氏佐剂混匀，注入家兔肩胛区皮下致敏，每只家兔每次注射1ml，于肩胛区5个不同区域注射，每周一次，连续注射3周而致敏，第4周向膝关节腔注射卵蛋白生理盐水溶液，每只关节腔注射5mg卵蛋白，15只家兔膝关节全部注射，24h内此关节直径和表面温度大幅度上升，以后缓慢下降，至14～21d达到平台期，发病率达100%。造模成功后第1～4周为早期改变，第4周以后出现不可逆的关节软骨及骨破坏。采用高频超声对RA模型组与对照组的膝关节髌上囊液体厚度、滑膜及股骨髁软骨厚度及软骨下骨皮质的回声情况进行对比观察。结果RA组模型组膝关节髌上囊积液及滑膜的厚度明显厚于对照组，其股骨髁软骨的厚度与对照组相比无明显差异，其软骨下骨皮质与对照组相比无明显改变。

These cells were then cultured in RPMI 1640 with 0.3% gelose for 10 to 14 days and the colonies were counted. LoVo cells and A549 cells were also inoculated subcutaneous in BALB/c nude mice.

将裸鼠皮下接种LoVo，A549细胞，72h后分组，每次注射20万IU/kg或100万IU/kg，500万IU/kg IFN-β，每天1次，连续10d，同时设置生理盐水对照组、标准品组和注射用环磷酰胺治疗组。

Purposes: To observe intakes of 〓p-sodium-orthophosphate and 〓P-chromium- orthophosphate at various concentrations by brain gliomas cell; To make thorough inquiry of the inhibition to the SHG44 cell by 〓P-sodium-orthophosphate and 〓P-chromium-orthophosphate at various concentrations; To find out the regularities about the accumulation and elimination in the major organs of rats after inter-brain injection with 〓P-sodium-orthophosphate and 〓P-chromic-orthophosphate; To investigate the therapeutic effects of intert-injection with 〓p-chromium-orthophosphate on the neoplasia transplanted in the brain and hypoderma; To estimate the absorbed dose of the neoplasia body in rats after internal radiation; To study the pathological changes in the rat cells and tissues caused by the radiation exposure from 〓p.

目的：观察人脑胶质瘤SHG44细胞对不同浓度的〓P-磷酸钠和〓P-磷酸铬的摄取；探讨不同浓度〓p-磷酸钠和〓p-磷酸铬对SHG44细胞生长的抑制；揭示〓p-磷酸钠和〓p-磷酸铬在大鼠颅内注射在时全身主要脏器的蓄积与排除规律；观察〓p-磷酸铬大鼠颅内瘤体、皮下瘤体内注射的治疗效果；估算大鼠瘤体组织的吸收剂量；观察〓p照射引起的细胞及组织的病理学改变，为临床脑胶质瘤内照射治疗提供实验依据和吸收剂量计算方法。

Result: 1. The method of arc-needle injection with 131I-iodinated oil step-by-step sector-shapely improved obviously the drug distribution, comparing with routine linearity injection. 2. SPECT image reconstruction detect 131I-iodinated oil three-dimensional distribution quantitatively. 131I-iodinated oil metabolism equation may obtained by continuous measurements, in order to calculate absorption dose of p particle at target. 3. ZT glue lengthens obviously 131I-iodinated oil retention in mouse local subcutis and rabbit liver which can rise local radiation effect, decrease injury at normal tissue.

结果：1、弧形针分段扇形注射~(131)I-碘化油，比常规使用的线性注射明显改善药物弥散均匀度。2、SPECT断层扫描定量检测~(131)I-碘化油的三维分布，连续测量建立~(131)I-碘化油的代谢方程，并计算~(131)I对靶区的β辐射贡献的吸收剂量。3、ZT胶明显延长~(131)I-碘化油在大鼠皮下、兔肝脏内的滞留。

In Rh2＋DDP group 1000uM cisplatine and 100uM gensenoside Rh2 were injected to nude mice by abdomen. Growth status of tumors were observed. Diameter of tumors were measured. After 60 days,〓Tc-MIBI were injected from vein of nude mice tail to perform SPECT exam and calculate the value R of the tumors. Later the nude mice were killed.

将A549DDP细胞和A549细胞分别种植于裸鼠皮下，种植A549细胞的裸鼠为A549组，种植A549DDP细胞的裸鼠分对照组、DDP组和Rh2+DDP组，每组4只裸鼠。A549组和对照组的裸鼠腹腔注射生理盐水，DDP组裸鼠腹腔注射低效浓度的顺铂；Rh2+DDP组裸鼠腹腔注射低效浓度的顺铂及无毒浓度的Rh2。

Simulate the condition of temperature and energy control in percutanous MWA for HCC to ablate SMMC-7721 cell strain suspension.There were five experiment groups:A.control group,B.45℃10 min group,C.50℃3min group,D.54℃3min group and E.60℃instant group.Detection for apoptosis was performed at 30 minutes and 2 hours after ablation.2.Thirty-two C57 mice were undertaken hypodermic inoculation of Hepal-6 tumor piece at right groin as ablation tumor and at left groin as observation tumor one week later.The mice with double tumors were separated into four groups:A.control group with no treatment,B.MWA group treated with MWA only,C.mDC group treated with MWA and subcutaneous injection of mature dendritic cells sensitized by neoantigen of Hepal-6 cell strain,D.iDC group treated with MWA and peritumoral injection of immature dendritic cells without neoantigen sensitization.

模拟肝癌微波治疗中常用消融区边缘的温控和能量条件，消融人肝细胞癌株SMMC-7721细胞悬液，分为五组：A、对照组，B、45℃10min组，C、50℃3min组，D、54℃3min组，E、60℃即刻组，消融后30分钟及2小时进行凋亡检测。2.32只C57小鼠左侧腹股沟皮下接种Hepa1-6鼠肝癌细胞瘤块作为消融瘤，间隔1周，右侧腹股沟皮下接种瘤块作为观察瘤，成瘤后分为四组：A组，对照组，不行治疗；B组，微波组，仅微波消融小鼠消融瘤；C组，成熟DC组，微波消融小鼠消融瘤，并于小鼠双侧腋下注射肿瘤抗原致敏的树突状细胞；D组，未成熟DC组，微波消融小鼠消融瘤，并于消融瘤周注射未成熟树突状细胞。

And then the next one that came through town, you might get close ups of rotting genitalia. You might get images of dead bodies. You might get a skinny-dipping scene.

而下一部到该城镇巡演的影片或许会让你看到腐烂的生殖器画面，或许是尸体，又或者是皮下注射画面。

Methods:According to their order of entering the hospital to divide all the 68 prematures into 34 cases in curing group and 34 cases in control group randomly, the curing group use rh-EPO (750 IU/kg) every week,every other day have a hyodermic for 6 weeks, along with the routine treatmentvitamin E 25mg/d,vitamin C (0.2 g/d, elenment iron 6mg/kg.d,and transfusion if it is possible,for the control group,only use the routine ...

将68例早产儿按入院顺序随机分为治疗组和对照组各34例，治疗组用rh-EPO每周750IU/kg，隔日1次，皮下注射共6周，加常规治疗，必要时输血，对照组仅用常规方法治疗。

ResultsIn the writhing test, 10 mg·kg-1 of Nudicaulol significantly reduced the number of writhing induced by ip 0.05% antimony potassium tartrate, in the hot plate test, 10 mg·kg-1 of Nudicaulol significantly enhanced threshold value of pain induced by thermal stimulation in 30, 60 and 90min after sc Nudicaulol.

均皮下注射给药。采用小鼠扭体法、热板法观察药后30 min各组小鼠的扭体反应次数和热板法药后30，60，90 min的痛阈值。结果10 mg·kg-1努地可醇可明显减少扭体次数、提高痛阈值，与生理盐水组比较差异（P＜0.01）。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。