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Methods:To develop the HPLC method for determination of Thymol and Carvacrol ,and compare the physico-chemical property.

高效液相色谱法测定百里香酚和香芹酚的含量,比较理化性质。

Result:The average recovery of carvacrol and thymol were 97\^49%( RSD =1\^78) and 99\^16%( RSD =2\^05) respectively.

结果 :香芹酚和百里香酚的平均加样回收率分别为 97 4 9%和 99 1 6 %,RSD =1 78和RSD =2 0 5。

The major components of thymus essential oil were thymol and p-cymene, and it exhibited higher antibacterial activity against Escherichia coli and Staphylococcus aureus.

百里香精油主要成分是百里香酚和对伞花烃,其对大肠杆菌和金黄色葡萄球菌具有较强的抗菌活性。

Results:23 components of thymus essential oil were separated and 9 of them were identified, the main constituents were thymol (44.36%) and p-cymene (31.04%); the diameters of inhibition zones of essential oil towards Escherichia coli and Staphylococcus aureus were 32 mm±0.5 mm and 40.5 mm±1.0 mm.

结果:百里香精油共分离出23个峰,鉴定出其中9种化学成分,主要是百里香酚(44.36%)和对伞花烃(31.04%);百里香精油对大肠杆菌和金黄色葡萄球菌的抑菌直径分别是32mm±0.5mm和40.5mm±1.0mm。

The detection wavelength was set at 274 nm.Result The linear range of thymol was 0.04~1.23 mg (r=0.9999),and the average recovery was 99.02%(RSD 1.03%).

结果 百里香酚在0.04~1.23 mg (r=0.9999)有良好的线性关系;平均回收率百里香酚99.02%(RSD 1.03%),重复性百里香酚RSD 0.88%。

The thesis applies the principle that organism and organic reagent can visualize in some condition and produce color association polymer. We have tried several organic reagents in order to build color reaction for berberine; at last, we find that thymol blue can react with berberine with good results.

Spectrophotometric Determination of Berberine using Thymol Blue-化学工程与工艺-利用有机化合物与有机试剂在一定的条件下能显色生成有色缔合物的原理,尝试了铬兰黑R、百里香酚蓝、甲酚红等多种有机试剂与盐酸小蘖碱的显色反应,结果发现麝香草酚蓝与盐酸小蘖碱的显色反应较好。

Methods: Through the reaction between protein and bromthymol blue, the absorption of the system was measured at 615 nm.

利用溴百里酚蓝与蛋白质的结合反应,在615nm波长处测定体系的吸光度。

The determination of MD1 concentration in aqueous solution by using bromthymol blue indicator method is described and a linear relationship is observed between absorbance and solution concentration ranged in 10-300 mg/L.

介绍了溴百里酚蓝为指示剂测定水溶液中MD 1质量浓度的方法,在10~300mg/L范围内溶液吸光度与浓度之间有良好的线性关系。

Bromthymol blue ; spectrophotometry ; protein

百里酚蓝;分光光度法;蛋白质

Two free radical scavenging capacity of the top three ranks are: thyme, ylang ylang, citronella oil; oil mostly contains polyphenols, a relatively high content of total phenols were the top three as follows: clove oil, thyme, benzoin.

两种自由基清除能力前三名排名皆为:百里香、依兰、香茅油;精油大多含有酚类物质,总酚含量相对较高者前三名为:丁香油、百里香、安息香。

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