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Geographical distribution characteristics of urban visibility in Anhui province are analyzed using routine observational data at 17 urban stations during the period from 1980 to 2005. The visibility trends in 5 cities from 1955 to 2005 have been analyzed using the methods of Ridit analysis cumulative percentiles and frequency of "very good" visibility.

利用安徽省17个地级市气象站1980-2005年的能见度观测资料,分析了安徽省城市大气能见度的地理分布特征,并用Ridit、累积百分率和"非常好"能见度出现频率分析法分别对5个代表性城市1955-2005年的大气能见度进行了研究和分析。

We have analysed the dynamic changes of the mount,cell phenotypes,cytotoxicity percentage of the PELs in differentdays induced by mIL-2+B16 and compared with its parent B16 cell's.

对mIL-2〓及其亲代〓细胞在不同天数内诱导的PEL的数量、PEL组成成分、对靶细胞的杀伤百分率等几个方面的特点进行了动态分析和比较,结果显示:1。

Understand the correlation and space distribution between the provenances that to apply the factor analysis with principal components solution and Cluster analysis.

本试验欲探讨种源之变异状况,并计算广义遗传率及遗传变方百分率,以了解遗传之特性;测验其生长性状之相关性,并利用线性回归,试图找出最适合台湾四湖海岸生长之种源及早期选拔之年龄;利用主成份因子分析做形态性状之综合评估,以了解种源之形态特性,并利用主成分因子分析和归群分析,了解种源间之空间分布及相互关系。

Results Analysis of the expression of proteins of JAK2 in leukemic blasts, there was no detectable constitutive expression of phosphorylated JAK2 in all the AML samples analyzed; Furthermore, we could detect a constitutive activation of STAT 3 by immunoprecipitation of the targeted protein in primary leukemic blasts from 3 of 11 AML patients without any cytokine treatment; Pretreatment clinical characteristics, and treatment regimens did not differ significantly between patients with and without constitutive STAT3 activity, there was not significant relation between the constitutive activation of STAT3 and the FAB subvariety and chromosome karyotype; However, the 3 patients with constitutive STAT3 activity seemed to have adverse treatment outcome, one did not show remission, the other two patients relapsed shortly after remission; Consistent with the above-mentioned result, leukemic blasts from the 3 samples with constitutive expression of STAT3 showed higher percentage of spontaneous apoptosis and S-phase cells.

在实验研究基础上,本研究进一步运用免疫沉淀和Western Blot印迹方法,检测AML患者原代细胞JAK2和STAT3活化情况,并分析了STAT3活化与AML患者预后有关指标改变的关系。结果我们的初步研究发现,11例AML患者中,有3例呈现STAT3组成型活化(STAT3阳性,下同),无一例呈JAK2组成型活化。上述3例STAT3组成型活化与FAB分型和染色体核型改变未见明显相关性;在此3例患者中,1例未取得缓解,另外2例虽然取得短暂缓解,但均在二个月内复发。上述3例STAT3阳性AML患者S期白血病细胞百分率数值较组高,而凋亡率数值较低。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法:1、培养原代的人RPE细胞,经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后,通过AO/PI染色技术确定培养细胞的存活率,描记其生长曲线,第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后,通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后,采用MTT法观察其对细胞的增殖力的作用;通过流式细胞仪观察其对细胞周期的影响;通过扫描电镜观察其对细胞形态的影响;通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用;采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率,评价其对细胞间通讯功能的影响;通过制作RPE细胞损伤模型,观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质,应用等电聚焦电泳和SDS-PAGE双向电泳技术,银染显示分离出的蛋白质斑点,经凝胶图像分析软件对两个样本进行胶图分析,寻找差异蛋白点。

Using the questionnaire made by myself, this study surveys and analyzes the actuality and demand of partial secondary school chemistry teachers in Chongqong, and then uses SPSS11.0 to analysis the redemptory questionnaire.

本文采取自编的《重庆市中学化学教师专业发展现状调查问卷》,对重庆市部分在职中学化学教师进行了问卷调查。然后用SPSS11.0统计软件对回收问卷进行统计分析,重点分析了频率、百分率和差异显著性检验(x2卡方检验)。

In this paper, The influence of heat set and dyeing on the structure of PTT fiber was investigated by FT-IR, DSC, critical dissolution time. The influence of heat set on the dyeability of PTT fiber was discussed, too. Meanwhile, many experiments on the dyeing performance of PET fiber were carried out. The effect of different dyeing temperature on the uptake and visual color depth value and dyeing characteristics of different dyes with temperature increasing were discussed, and the influence of temperature on the dyeing depth were also evaluated when dyeing for PTT and PET fiber in the same dye bath condition. Especially the kinetics and thermodynamics of dyeing with disperse dyes were mainly studied.

目前,我国对PTT染色性能方面研究报道还少,本文用傅立叶红外光谱、差热分析、临界溶解时间等分析方法研究了热定型和染色对PTT纤维结构的影响,探讨了热定型对PTT纤维可染性的影响,同时进行了多项PTT纤维的基本染色性能试验,探讨了不同染色温度对上染百分率及表观色深值的影响、不同染料的升温上染特性、不同染料的移染性能和染深性能以及PTT和PET纤维同浴染色时染色温度对它们的染色深度的影响。

Methods The expression of MMP-2 and TIMP-2 were observed by immunohistochemical technique in 57 cases of cervical carcinoma and 17 cases of normal cervices.

采用免疫组化S-P法对57例宫颈癌组织、17例正常宫颈组织中MMP-2、TIMP-2进行染色,在光学显微镜下观察,并根据阳性百分率进行分级,采用x~2检验及等级相关分析进行数据分析。

When the cells have adhered to coverslip after being cultured for 24h, we use 70% ethanol to fix the cells for 20 minutes standby.

CD133表达流式细胞仪分析:分析对数生长期的骨肉瘤细胞系Saos-2细胞的CD133表达百分率。

To analyze the reliability and comparability of different hematology analyzers and to standardize the operation of a contrast test. Methods The carryover rate, precision, total precision and percentage of differences of hematological analyzers were evaluated and differences of items in whole blood were compared by detecting 20 anticoagulant fresh blood samples based on the documents of ICSH and NCCLS. Every 3 blood samples of highvalue, middlevalue and lowvalue were detected by hematological analyzers everyday for 7 days.

方法参照国际血液学标准化委员会及美国临床实验室标准化委员会文件,在仪器正常运行的情况下,评价各比对仪器的携带污染率、精密度和总精密度、差异百分率;分析比较20份抗凝新鲜全血中各项指标在不同血细胞分析仪之间的差异;每日取高、中、低3份新鲜血,在各仪器上平行检测,连续比对7日,观察各项指标与参考样机之间的相关性;分析比较仪器分类与手工分类的相关性;通过直线回归方程计算相对偏差,估计每台仪器各检测指标的试验误差。

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Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了

The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.

世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。