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Five polymorphic primers with good, clear bands were got, screening fromseventy-five ISSR primers. These five pairs of primers were used to amplificaterespectively the genomic DNA of 66 different Pterocarya stenoptera individuals and atotal of 45 bands were amplified with 43 polymorphic bands and percentage ofpolymorphic bands 95.6%.

2从75条ISSR引物中筛选出5条多态性好、谱带清晰的引物,用这5条引物对66个枫杨叶样的基因组DNA进行了PCR扩增,共得到45条带,其中多态性带43条,多态性百分率为95.6%。

In view of it, the author analyses the climatic change in Dalian and comes to a series of conclusions: the climate has the trend of warming and rainless; the average ...

采用气温线性增长率与距平百分率等方法进行分析后,得出近五十年来大连市气候有向暖干化发展的趋势;年平均气温有上升趋势,而年降水量有下降趋势;冬季气温对年均气温拉动作用明显,而夏季降水量减少对年降水量减少贡献率最大等结论。

Using the questionnaire made by myself, this study surveys and analyzes the actuality and demand of partial secondary school chemistry teachers in Chongqong, and then uses SPSS11.0 to analysis the redemptory questionnaire.

本文采取自编的《重庆市中学化学教师专业发展现状调查问卷》,对重庆市部分在职中学化学教师进行了问卷调查。然后用SPSS11.0统计软件对回收问卷进行统计分析,重点分析了频率、百分率和差异显著性检验(x2卡方检验)。

The phagocyte swallows saccharomycetes 20 minutes after to swallow the percentage to be possible to reach as high as 86%.

结果显示小鼠巨噬细胞吞噬酵母菌20min的吞噬百分率可高达86%,说明这种改良法简单而有效。

Results Experimental group,the phagocyte swallows saccharomycetes 10 minutes after to swallow the percentage to be possible to reach as high as 57%.

结果 实验组吞噬10min的结果吞噬百分率57%,吞噬20min的作者:孙明洁,高燕,赵冬,王珂,靖学芳

Results Experimental group,the phagocyte swallows saccharomycetes 10 minutes after to swallow the percentage to be possible to reach as high as 57%.

结果 实验组吞噬10min的结果吞噬百分率57%,吞噬20min的结果作者:孙明洁,高燕,赵冬,王珂,靖学芳

Result: The absorption rates in rat stomachs at 2 h after administration was 76.31%; The absorption rates at colon, duodenum, ileum and jejunum were 46.25%, 40.54%, 38.21%, 32.77%, respectively. The absorption rate constant at concentrations of 10.014 4, 20.028 8-40.057 6 mg·L-1 in intestine were 0.643 4, 0.613 7, 0.597 0 h-1, respectively. The K a of scopoletin at pH of 6.0, 6.8 and 7.4 in intestine were 0.621 7, 0.603 3, 0.613 7 h-1, respectively.

结果:东莨菪素在大鼠胃中2 h的吸收百分率为76.31%;东莨菪素在结肠、十二指肠、回肠、空肠的吸收率分别为46.25%,40.54%,38.21%,32.77%;不同质量浓度东莨菪素10.014 4,20.028 8,40.057 6 mg·L-1在小肠的吸收速率常数分别为0.643 4,0.613 7,0.597 0 h-1;不同pH(6.0,6.8,7.4在小肠的吸收速率常数分别为0.621 7,0.603 3,

The survival rates of CEM cells cultured with cis-diamminedichicloroplatinum added 24 h later were higher than that cultured with hTERT ASODN and DDP added 24 h later. The survival rates of CEM cells cultured with DDP were similar with that cultured with hTERT SOND and DDP. In morphological observation of apoptotic cells using Giemsa staining, cells treated with DDP or DDP combined with hTERT ASODN ro SODN at 48 h, displayed classic apoptotic changes. Apoptosis rates of CEM cells treated with DDP for 48 h after 24 h of exposure to ASODN significantly increased. There was significant difference in the percentage of apoptotic cells of CEM cells between hTERT ASODN plus DDP and SODN plus DDP or DDP alone, respectively.

结果: hTERT ASODN作用于CEM细胞24 h再加入柔红霉素、长春新碱、足叶乙甙,对细胞生长的抑制分别与单用柔红霉素、长春新碱、足叶乙甙及hTERT正义寡核苷酸联合柔红霉素、长春新碱、足叶乙甙组相比,统计学上无显著差异(P>0.05)。hTERT反义核酸作用于CEM细胞24 h加入顺铂,再共同作用48 h,CEM活细胞均数为2.318×108 cells/L,与单用顺铂组(3.250×108 cells/L)及hTERT正义核酸联用顺铂组(3.175×108 cells/L)相比,对细胞抑制明显增强(P<0.05)。hTERT ASODN作用于CEM细胞24 h再加入顺铂作用48 h,细胞出现典型的凋亡形态学改变。hTERT ASODN与2.5 μmol/L顺铂联合作用于CEM细胞48 h的凋亡细胞百分率(19.47%)分别同SODN与顺铂联合作用组(6.97%)、单用顺铂作用组(6.02%)进行比较有显著差异(P<0.01)。

Get the neonate Sprague-Dawley rats medulla spinalis, through the conventional primary cell culture procedure, we use the method of difference adherence time and get the motor neurons of anterior spinal cord. When the neuron is in maturity, we make the mechanical injury model in vitro. All the models were divided into four groups: group A is control group; group B is 100μM ATP group; group C is 100μM ATP+20μg/ml suramin group and group D is 100μM ATP+10μM ouabain+10μg/ml Thapsigargin group. Culture the four groups neurons, after one day, we count the motor neuron, observe the survival and activity of neurons through MTT shade selection experiment, use flow cytometry to analyze the percentage of apoptosis of motor neurons of anterior spinal cord and detect the expression of protein p-GSK-3β(ser9) through Western-Blot technology.

方法取新生大鼠脊髓,通过常规的原代细胞培养程序,采用差速贴壁法分离出大鼠脊髓前角运动神经元,培养成熟后制作神经元机械损伤体外模型,分为A组:对照组、B组:100μMATP组、C组:100gMATP+20μg/ml Suramin组和D组:100μM ATP+IOμM Ouabain+10μg/ml Thapsigargin组,对各组机械损伤的运动神经元进行培养,1天后分别进行运动神经元计数、MTT比色实验观察运动神经元的存活及活性、流式细胞仪分析机械损伤的脊髓前角运动神经元凋亡百分率和Werstern Blot技术检测p-GSK-3β(ser9)的表达。

With the daily radiation data and monthly data of mean temperature, precipitation, relative humid, sunshine fraction and wind velocity amended Penman's method was used to calculate the potential evapotration spiration and the aridity index, at present and three prescribed climate scenarios, with temperature rise by 1.5℃, 3.0℃ and 4.5℃, and precipitation increase by 10%.

中国气候信息库提供了全国太阳辐射日资料以及12个月的气温、降水量、相对湿度、日照百分率和风速资料。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。