百分率

Using 11 primers filtrated from total 53 primers, ISSR amplification was conducted in 220 individuals from eleven Cryphonectria parasitica subpopulations which were from four countries, and 174 polymorphic loci were detected from total 177 loci. The percentage of polymorphic loci of the total populations was 98.31%, while that at a subpopulation level averaged 20.43 %. Shannon's Information index and Nei's gene diversity of the fungus at the total populations level was 0.1769 and 0.7386, respectively.

自53个ISSR引物中筛选出11个引物，对以上四个群体的栗疫病菌11个子群体的220个菌株进行扩增，共检测到177个位点，其中多态位点为174个，多态位点百分率为98.31%。11个栗疫病菌子群体的P平均值为20.43%；群体水平的Shannon信息指数和Nei指数分别为0.1769和0.7386；各个子群体I的平均值为0.0943，h的平均值为0.0614。

The analysis of lymphocyte count in peripheral blood has certain guiding significance in pathogenetic of many malignant tumors.

血象中淋巴细胞百分率和淋巴细胞绝对值分析能够对肺癌等恶性肿瘤的发病机理起一定的指导意义。

The result indicated that: the amount of the excess HF formed direct ratio to the yield of phosphorus pentafluoride and the optimum amount of the excess HF used is about 35%; the trickling rate of oleum shouldnt make the temperature of hexafluorophosphoric acid exceed 25 ℃ and the amount of oleum is 110% to 125% that of stoichiometry so that the content of phosphorus pentafluoride can be achieved 90%; the time and temperature of heating hexafluorophosphoric acid influenced both the yield and the content of phosphorus pentafluoride, and the optimum time should be about 30 minutes and the proper temperature was 148 to 153℃; when alcohol used asrefrigerant, the content of phosphorus pentafluoride was 94.03%, and the content can be up to 97.38%, provided that using liquid nitrogen as refrigerant.

通过实验发现，氟化氢用量与五氟化磷的产率成正比，最佳过量百分率宜在35％左右；发烟硫酸的滴加速度不能使中间产物温度大于25℃，发烟硫酸用量大于理论量10％～25％时，得到的五氟化磷纯度高于90％；六氟磷酸的加热温度对五氟化磷的产率和纯度均有影响，宜在148～153℃的范围内，加热时间应在30分钟左右；用工业酒精做冷凝介质得到的五氟化磷纯度最高只能到达94.03％，使用液氮做冷凝介质可以使五氟化磷的纯度达97.38％以上。

Pathological observation of the lung of asthma control showed increase in mucous secretion in alveolar space and peribronchial infiltration of large amount of inflammatory cells, primarily EOS and lymphocytes.

哮喘对照组肺组织肺泡腔黏液分泌增加，支气管及血管周围大量炎性细胞浸润，以EOS、淋巴细胞为主，与正常对照组比较，BALF中细胞总数、EOS数目和百分率均明显增加(P.01)。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

Results 5-FU inhibits growth of Lovo cells; it is found that within a limited dosage and period of time, there has been an obvious increase in apoptosis cells under the photomechanical scope; the analysis of Lovo cells by FCM showed that after the induction of 5-FU, the percentage of apoptosis cells has increased; and FCM also displays dosage and time effects. Electrophoresis was obviously in a stripe shape of trapeziform.

结果 5－FU能抑制 Lovo细胞生长，在一定剂量和时间范围内，通过光镜观察，见凋亡细胞明显增多，经5－FU诱导后，用FCM 分析 Lovo细胞，发现凋亡百分率增加并显示剂量和时间效应。

The reduction of the number of propagative floweret resulted in decrease of the differentiae floweret, but no decrease of the percentage of floweret deterioration.

而可孕小花数下降并非发育小花退化百分率下降所致，而是发育小花数下降造成。

The prototypic IL-15 gene transfection could enhance the ability of NCI-H446 cells to induce PBMC to proliferate and to kill the wild type NCI-H446 cells.

结果 与MCw—PBMC相比，MCp-PBMC在细胞数、CD4^＋细胞和CD8^＋细胞百分率及对Cw的杀伤上，均显著提高（P〈0.05）。

T lymphocyte percentages of peripheral blood, lymph node, spleen, thymus and bone marrow of maters of interspecific, intraspecific embryo implantation and pseudopregnant mice at corresponding time were measured using fluorescence labeled monoclonal antibody and flow cytometry.

利用荧光标记的单克隆抗体染色结合流式细胞术，检测种间、同种胚胎移植以及同时期假孕母体外周血、淋巴结、脾脏、胸腺、骨髓中T淋巴细胞的百分率。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。