白血病的

This study was purposed to investigate the possible side effects of L-asparaginase in the treatment of patients with acute lymphoblastic leukemia and to explore the corelation of these side effects at different therapeutic stages by means of retrospective analysis, so as to reduce the incidence of side effects and improve the safety of chemotherapy and the long-term survival of patients.

本研究采用回顾性方法分析左旋门冬酰胺酶在儿童急性淋巴细胞性白血病治疗过程中可能发生的副作用，以探索L-ASP副作用的发生与患儿所处治疗阶段的关系，以便采用有效的监测手段减少副作用的发生，提高化疗安全性及患儿的长期存活率。

This study that observed the expression and distribution of 4 apoptotic factors, relation apoptotic factor with apoptosis, changes of T and B subgroup, and changes of molecular pathology in 14 cows suffering from enzootic bovine leucosis from several resides was detected by histochemistry, TUNEL technology, immune-fluorescent flow cytometry and so on. The aim of this experiment supplies theoretic basis for preventive and therapy of EBL, and consult dates for T lymphoid cell leucosis of human being .

本研究检查并剖检了14例临床与实验室诊断为EBL的病牛，观察分析了肿瘤发生的情况和特征，采用免疫组织化学ABC法、原位末端标记TUNEL技术和免疫荧光流式细胞检测法等技术，从EBL肿瘤细胞凋亡特性入手，围绕4种凋亡因子和几种免疫相关因子与EBL肿瘤发生的关系及其在诊治方面的应用进行了首次有价值的探索，其目的在于为防治EBL提供科学的理论依据，同时，也将为人类T细胞白血病Ⅰ。

The study was purposed to investigate the effect of extract of Agkistrodon Halys venom on proliferation and apoptosis of K562 cells. The inhibition of K562 cell proliferation was measured by MTT assay; The morphologic changes of K562 cells was observed by microscopy; the apoptosis of K562 cells was measured by flow cytometry; the activity of extracellular signal-regulated kinase in K562 cells was detected by Western blot.

本研究探讨皖南蝮蛇毒蛋白提取物对白血病细胞K562增殖的影响及诱导凋亡的作用，采用MTT法检测不同浓度的蛇毒蛋白提取物对K562细胞生长的影响；应用电子显微镜观察药物作用后K562细胞形态的改变；流式细胞术检测蛋白提取物对K562细胞细胞凋亡作用；Western blot检测细胞增殖酶活性的变化。

The hydroxy and nitro-substituted coumarins were able to inhibite hemolysis of human red blood cells and damage of DNA induced by AAPH. It reveals that these compounds are effective antioxidants. Some coumarins have cytostatic and cytotoxic activity for HL-60 cell lines. In general, their effects were weakened when coumarins own nitro group.

含有羟基和硝基的香豆素衍生物对自由基诱导的人血红细胞溶血有一定的抑制作用，且对AAPH诱导的DNA损伤有不同程度的保护作用，部分香豆素衍生物对人早幼粒白血病细胞（HL-60）的增殖表现出一定的抑制作用。

During these years (1997-2000) in the research area of annonaceous acetogenins 5 natural products and a number of chiral-pure analogs of annonaceous acetogenins have been synthesized using sugars as the starting materials. Their high activity and selectivity against cell line of colon tumor and a relationship between activity and configuration have been found too. In the respect of inhibitor of sialidase two new methodologies for the synthesis of chiral-pure 3-deoxy-ulosonic acid using hetero-Diels-Alder reaction or asymmetric propargylation as the key reaction were developed. On the topic of anti-leukemia marine natural product clavulactone a synthetic method based on the free radical cyclization and starting from sugar has been developed. In other title of PKC inhibited agent sphingosine a new synthetic route with SN2' as the key reaction has also been disclosed.

项目期间（1997-2000）在番荔枝内酯方面从糖出发合成了五个天然产物和一批手性纯的类似物，它们对肠癌细胞有很高的活性和选择性，并与构型有关；在唾液酸酶抑制剂方面发展了二类全新手性纯高碳糖的合成方法－杂原子D－A反应或丙炔基不对称加成为关键的反应；抗白血病海洋天然产物群柱虫内酯方面研究了由糖出发和自由基环化反应的合成方法；对PKC 起抑制作用的鞘氨醇方面也开创了新的合成途径－由糖出发并以SN2'反应为关键反应。

Our data are showed that apoptosis parameters including DNA fragmentation, nuclear morphological change and sub-G1 fraction, appeared after 24 h by 5 μM rottlerin treatment. Furthermore, rottlerin caused a decrease in 72% of ODC protein expression and an increase in threonine, rather than serine and tyrosine dephosphorylation on ODC enzyme compared with the control after incubation for 10 h. To determine whether ODC could down-regulate rottlerin-induced apoptosis, HL60 cells were stably transfected with ODC cDNA. HL60-ODC cells exhibited a 2.5-fold increase in ODC protein compared with vector only (HL60-pcDNA3) or HL60 cells.

在我们筛选天然化合物实验中，发现rottlerin抑制鸟胺酸去羧化活性最具成效，之前的研究人员已知蛋白激Cd调节鸟胺酸去羧化的活性，因此在人类白血病HL60细胞处理rottlerin的实验中发现加入rottlerin二十四小时后，细胞进行计划性死亡，并可侦测到DNA片段、细胞核浓缩及sub-G1提增；而细胞处理药物十小时后与控制组细胞比较，观察到鸟胺酸去羧化的蛋白表现与活性下降50﹪以上；除此以外，测定鸟胺酸去羧化去磷酸化的情况，发现於酥胺酸位置有显著差异，而丝胺酸和酪胺酸位置并无差异。

The treatment of leukemic cell with a longterm, intermittent,singular, unefficient chemotherapeutic agents could induce multi-drug resistant cells. Interleukin-4 can reverse the multi-drug resistance by inhibiting the expression of p-gp and increasing the quantity of intracellular chemotherapeutic agents in tumor cells. The effects were probably related to the inhibition of the PKC activity.

长期、间断、单一、不足量的化疗可导致 MDR；IL-4 可通过抑制肿瘤细胞的 p-gp表达，增加化疗药物在肿瘤细胞内的聚集而逆转白血病细胞的MDR，此作用可能与抑制 PKC的活性有关。

Won't! The doctor of children hospital lets my Mom go with my wife haemal institute, go checking an examination, my Mom and my wife also went, the result of the examination and two results before are same, acute leukaemia, after my Mom and my wife come home, said the condition to us to listen, our family stayed, how can you contract this kind of disease?

不会的！儿童医院的医生让我妈跟我老婆去血液研究所，去检查检查，我妈和我老婆也去了，检查的结果和之前的两个结果是一样的，急性白血病，我妈和我老婆回家以后，把情况说给我们听了，我们一家人呆了，怎么会患这种病？

Researchers showed that children who attended daycare or playgroups have a 30 percent lower risk of developing the most common form of childhood leukemia compared to those who did not.

研究人员显示，与其他的孩子相比，日托的孩子或者打堆一起玩的孩子，发生最常见形式的儿童白血病30%的低风险。

P210bcr/abl plays a central role in the pathophysiology of CML. The purpose of this study was to construct a cell line model that bcr/abl expression can be regulated by Tetoff inducingexpressionsystem. The fulllength b3a2 bcr/abl cDNA was subcloned into the pTRE2hyg expression vector to construct the pT2P210 plasmid.

CML是目前致病分子信号基础研究最为清楚的肿瘤之一，1960年Nowell和Hungerford在CML患者的白血病细胞中发现Ph染色体［1］，1973年Rowley等［2］进一步应用染色体分带技术证明，Ph染色体是由9q34的abl基因和22q11 的 bcr 基因相互易位形成的 bcr/abl 融合基因。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。