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Methods: Nonadherent cells from bone marrow or peripheral blood mononuclear cells of 8 patients with chronic myeloid leukemia were cultured with rhGM- CSF and rhIL- 4. The cytokines were supplemented every three days. RhTNF-α was used at the ninth day. All the cells were harvested at the twelfth day.

从8例慢性髓性白血病患者的骨髓或外周血中获取非贴壁细胞,利用细胞因子(rhGM蛳CSF、rhIL蛳4、rhTNF蛳α)联合培养,每3 d补充新的细胞因子,其中rhTNF蛳α为第9天添加,第12天收获细胞。

HAP nanopartics may provide a brighter prospect in treatment for leukemia and other oncologic disorders.

HAP纳米粒子对白血病及肿瘤的治疗将具有良好的开发应用前景。

At present,the pathogenesy of leukemia is not very clear,which will achieve more recognition with the development of modern empirical method.

目前,白血病发病机制尚不完全清楚,随着现代实验方法的进步,人类对其发病机制有了逐渐的认识。

This method also helps to subclassify cell types, which may, in turn, help the physician to decide on the best treatment to apply in that type of leukemia or lymphoma.

种方法也能够帮助区分细胞亚型,可帮助内科医师制定最佳的治疗方案用于不同类型的白血病或淋巴瘤。

Normal lymphocytes may be distinguished from leukemic lymphocytes. This method also helps to subclassify cell types, which may, in turn, help the physician to decide on the best treatment to apply in that type of leukemia or lymphoma.

这种方法也能够用以区分细胞亚型,可帮助内科医师针对不同类型的白血病或淋巴瘤制定最佳的治疗方案。

When HIV-1 in culture medium was exposed to a range of concentrations of urokinase, a dose-dependent inhibition of infectivity was observed in a variety of lymphoma and leukemia cell lines including MT4, CEM, H9, and peripheral blood mononuclear cells using assays for reverse transcriptase, P24 antigen and syncytia formation.

通过检测逆转录酶活力,P24抗原的表达和合胞体形成情况发现尿激酶可以抑制人体免疫缺损病毒对多种淋巴瘤和白血病细胞系,如MT4、CEM、H9和外周血单核细胞的侵染能力,并且这种抑制与尿激酶浓度呈剂量依赖关系。

And TOPOⅡα in de novo acute leukemia and explore the relationship between its expression and chemotherapy efficacy and prognosis.

拨?和拓扑异构酶Ⅱα在初发急性白血病患者中的表达,并探讨其与疗效及预后之间的关系。

Infection of ESCs by the murine leukaemia viruses results in the normal establishment of integrated proviral DNA, but this DNA is then transcriptionally silenced, preventing further viral spread.

通过鼠类白血病病毒感染ESCs导致整合前病毒DNA的正常建立,但这种DNA而后变为转录沉默,阻止了病毒的进一步蔓延。

To elucidate the possible mechanism of differentiation and /or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor, the level of acetylated histone H3 was detected by Western blot, p21~ WAF1expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin,TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制,利用Westernblot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21WAF1表达量的改变。

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin, TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制,利用Western blot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21(上标 WAF1)表达量的改变。

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