白血病

The study was purposed to investigate the effect of deguelin on expression of nucleoporin 98(nup98) in leukemia K562 cells.

本研究观察鱼藤素对白血病细胞株K562细胞核孔蛋白nup98表达的影响。

Objective To investigate antitumor activity and molecular mechanism of deguelin to the human U937 leukaemia cells and to explore the mechanisms regulating cell cycle and nucleoporin 98 （Nup98） and nucleoporin 88 （Nup88） in vitro.

目的研究鱼藤素对人类白血病细胞株U937细胞体外抗肿瘤作用，研究其引起细胞周期与核孔蛋白Nup98和Nup88的改变，探讨其抗癌作用的分子机制。

The expression levels of Nup88, Nup214 and NPM were decreased in a dose-dependent manner both at protein level and transcription level when cultured with deguelin.

其抗白血病效应可能与其下调核孔蛋白Nup88、Nup214以及核磷蛋白NPM的表达有关。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人类T淋巴细胞白血病细胞系Jurkat作为研究对象，采用MTT法检测细胞增殖活性；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI双标法检测细胞凋亡；流式细胞术、Western Blot、RT-PCR检测鱼藤素作用前后，Jurkat细胞内核孔蛋白Nup88、Nup214与核磷蛋白(nucleophosmin， NPM)表达水平的变化；激光共聚焦显微技术观察上述核孔蛋白与核磷蛋白的亚细胞定位情况。

Methords After the KG1a cells had been treated by the demethylation kit, the DNA was drawn.

去甲基化试剂盒对急性髓性白血病细胞KG1a进行去甲基化处理，并提取其DNA。

Other oral disorders such as Candidiasis, Desquamative Gingivitis, Vesiculobullous diseases, acute monocytic leukemia, hematological disorders and nutritional problems all respond to aloe vera use.

其他疾病如口腔念珠菌感染、牙龈炎脱屑、水疱病、急性单核细胞白血病、血液病、营养问题都回应芦荟使用。

Key words diallyl disulfide; HL-60 cell; leukemia; vascular endothelial growth factor

本研究初步探讨DADS对HL-60白血病细胞株抑制作用的可能机制。

Objective To study the effects of diallyl trisulfide on proliferation, apoptosis and cell cycle in acute myeloid leukemia cells HL-60 and the probable mechanism involved, aiming at offering credible academic and experimental bases for clinical use.

目的：研究二烯丙基三硫对急性髓细胞系白血病细胞株HL-60细胞增殖、凋亡及细胞周期的影响并探讨其作用机制，为二烯丙基三硫用于临床防治癌症提供可靠的理论基础和实验依据。

Objective:To study the proliferation and expression of survivin protein in human leukemia HL-60 cell line treated with diallyl disulfide and to thus investigate the mechanism of DADS to inhibit HL-60 cells' growth.

目的：观察二烯丙基二硫(diallyl disulfide，DADS)作用于人白血病HL-60细胞株后，细胞增殖及survivin蛋白表达的变化，探讨DADS对HL-60细胞增殖的影响及其机制。

Objective To investigate the functional mechanism of Chinese drug disodium cantharidinate reversing multidrug resistance of tumor cells.

目的 观察斑蝥酸钠对多药耐药的白血病细胞(K562/AO2)细胞是否有逆转作用，并初步探讨其逆转机制。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。