白蛋白

Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上，开展了如下未见文献报道的创新性的研究工作：(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究，建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法，与以前的报道相比，灵敏度和分析时间均有很大改善；(2)使用荧光素钠作为背景荧光试剂，建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法，扩大了激光诱导荧光检测的应用范围，对难衍生化合物的分离分析提供了一种新思路和新途径；(3)以荧光素钠作为背景荧光试剂，建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法；(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物，为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Estrone (E1) was bound to bovine serum albumin to get a complete antigen (E1-BSA).

雌酮与牛血清白蛋白共价结合，合成雌酮的完全抗原。

Screening data were collected on urinary albumin excretion and risk factors for cardiovascular and renal disease.

并且收集了参与者尿白蛋白排泄率以及心血管和肾脏疾病的危险因素的筛选数据。

objective to study the role of urinary albumin excretion rate and glomerular filtration rate for diagnosis of early diabetic nephropathy.

目的 探讨尿白蛋白排泄率和肾小球滤过率在早期糖尿病肾病的诊断价值。

Objective To establish a radioimmunoassay method for determination of urinary albumin excretion in rats and to evaluate its significance.

目的 建立大鼠尿白蛋白放免测定方法及其应用意义。

objective to observe the effect of benazepril in decreasing the urinary albumin execration rate and protecting the renal function in normotensive early diabetic nephropathy.

目的 观察贝那普利对血压正常的早期糖尿病肾病患者尿微量白蛋白的排泄和肾功能的影响。

Ambs S,Dennis S,Fairman J,Stillman IE,Lombardo M.Inhi-bition of tumor growth correlates with the expression level of a human angiostatin transgene in transfected B16F10melanoma cells ［J］.

本实验将大鼠血清白蛋白分泌信号正确连接在endostatin基因的碳末端，成功构建了真核表达载体pcDNA3-SE，并利用Lipofectamine介导转染C6胶质瘤细胞。

To study the surface treatment of the quartz glass, and the coupling of the albumin protein, we used with the fictionalization of the copolymers, and experimental conditions for protein immobilization.

蛋白质固定化之制作程序系将白蛋白固定於涂有光阻剂共聚物之石英玻璃表面，然后检测蛋白质於280 nm波长附近的吸光特性，以评估蛋白质固定化的效果。

"The abbot, in his alb arrayed," stood at the altar in the abbey-chapel of Rubygill, with all his plump, sleek, rosy friars, in goodly lines disposed, to solemnise the nuptials of the beautiful Matilda Fitzwater, daughter of the Baron of Arlingford, with the noble Robert Fitz-Ooth, Earl of Locksley and Huntingdon.

"在他的住持白蛋白，排列，"站在了修道院，教堂祭坛的Rubygill，与他所有的丰满，光滑，红润修士，在优美的线条处理，以solemnise美丽的明德菲茨沃特，对女儿的婚礼伯乐阿灵福，与高尚罗伯特菲茨-切牙，亨廷顿的洛克斯利和厄尔。

Congregation obtained after chitosan k and a treatments showed more tightness than sediments obtained without chitosan treatment, sediments after chitosan b and c treatments were network in structure. Mixed systems of pectin, protein and polyphenol were applied for studying a simulated system of kiwifruit juice. It was shown that the aggregates of pectin molecules in pectin solution were dispersing tubular globules. Particles in mixed solution of pectin and bovine serum albumin had two states: one was circular in photo, the other contained single particle or aggregates of several particles. Pectin decreased the interaction between protein and polyphenol and strengthened the stability of mixed solution of pectin, protein and polyphenol. Chitosan flocculated mixed solution of pectin, protein and polyphenol, and the floccule was white, having a network structure.

用果胶-蛋白质-丹宁酸混合体系作为猕猴桃原果汁的模拟体系研究结果表明：1、果胶溶液中果胶分子聚集物呈中空球形均匀分散；2、果胶／牛血清白蛋白混合溶液中的球形粒子有两种：粒子中央光线可透过，照片上呈环形；粒子的中央包含有单个或多个球形粒子的聚集体，认为粒子的中央是蛋白质，外层是果胶；3、果胶的存在抑制或减弱了蛋白质-丹宁酸之间的相互作用，果胶起到防止蛋白质-多酚产生沉淀的作用，增强了溶液的稳定性；4、壳聚糖加入果胶-蛋白质-丹宁酸混合溶液中时，可以絮凝果胶-蛋白质-丹宁酸混合溶液，溶液中出现白色的网状絮凝物。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。