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Biocell Laboratories, Inc has four decades of expertise in the fractionation and purification of human and animal serum proteins and is currently the only U.S. producer of bovine serum albumin and bovine gamma globulin utilizing the Cohn Cold Ethanol Precipitation Process.

Biocell实验室,公司在人类和动物血清蛋白的分馏和提纯上有四十年的经验,目前是美国利用科恩的冷乙醇沉淀法生产牛血清白蛋白和牛丙种球蛋白唯一生产商。

ALT, AST, serum albumin, globulin in serum and SOD, MDA in liver and liver histological changes were measured to confirm the ability of protecting liver of Danxiongfang.

结果:丹芎方和各组分均可抑制CCl4损伤后血浆中ALT和AST的异常增高,提高血清和肝脏中SOD活性,减少MDA形成,升高血浆中总蛋白、白蛋白水平,减轻肝脏疏松化及脂肪变性。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法:在体外,应用细胞培养的方法,培养SMMC-7721肝癌细胞系,分为试验组和对照组,试验组加入不同浓度Se、I或T3干预,培养一段时间后,通过噻唑蓝试验、流式细胞仪,观察细胞数量及细胞周期的变化,测定细胞培养上清液中甲胎蛋白、白蛋白的水平,观察细胞分化情况;在体内,将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组(为Se、I和T3混合物),首先预防性用药2周,然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞,制造腹水癌大鼠模型;继续用药2周,观察各组大鼠发病率及死亡率,测量不同时期大鼠血清FT3含量,通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

Alb, Che and PA were examined by end-point method, enzyme kinetics and immunologic projective turbidimetry method respectively.

血浆清蛋白为终点法、胆碱酯酶为酶动力学法、及前白蛋白免疫投射比浊法。

No significant changes in the protein signals were observed, except that the signal from albumin lysyl shifted towards upfield.

此外,没有观察到血清蛋白信号的明显变化,仅血清白蛋白赖氨酰信号随pH增高有高场位移。

The initial ascitic fluid analysis should include serum ascites-albumin gradient in preference to ascetic protein.

腹水的初步分析应该包括血清腹水-白蛋白梯度并要优先于腹水蛋白。

The high MELD score, low serum albumin and ascitic fluid total protein levels were independently associated with a greater risk of SBP.

较高的MELD评分、较低的血清白蛋白及腹水总蛋白浓度3者可能是肝硬化腹水患者并发SBP的独立高危因素。

After Bel-7402 was treated with melittin, detection methods were applied as followed, 1 measure the change of proliferation by MTT ; 2 light microscope and transmission electron microscope were used to observe the morphous and biological behaviour and ConA agglutination test and clonality test were applied to measure the adhesion capability and growth potency of Bel-7402; 3 ELISA , colorimetry , bromocresol green mehod were used to determine AFP level, the activity of y - GT and ALP, ALb level respectively; 4 the change of c-myc gene expression was detect by immunohistochemical method.

观察蜂毒素作用于人肝癌Bel-7402细胞株后(1)MTT方法测定对肿瘤细胞增殖的影响(2)光学显微镜和透射电镜下观察对形态及生物学行为的影响,ConA凝集试验及克隆形成能力反映处理后细胞粘附能力及生长潜能(3)ELISA法测定甲胎蛋白含量、重氮反应比色法测定γ-谷氨酰转肽酶活力、比色法测定碱性磷酸酶活力、溴甲酚绿法测定白蛋白含量,检测对肝癌细胞代谢与分泌的影响(4)免疫组织化学法检测处理前后c-myc基因表达的差别。

Serum alt,ast,and bile acid were observed by enzyme assay and the ratio of ast/alt was worked out. serum bilirubin was measured by peariman and lee method. serum albumin was tested by bromphenol assay and was simultaneously measured to hyaluronic acid, type ⅲ procollagen, laminin and type ⅳ collagen by ria. meanwhile, prothrombin time and platelet count were measured and so on. results: the level of serum aca was raised increasingly in different cirrhotic cases with different classifications of child-pugh, according to severity of the condition.

应用酶联免疫吸附法分别对不同分级的肝硬化患者血清中抗心磷脂抗体的含量进行了测定;用酶法测定了alt、ast与胆汁酸并计算ast/alt值;用peariman和lee改良法测定血清胆红素;用溴甲酚氯比色法测定血清白蛋白;用放射免疫分析法测定透明质酸、ⅲ型前胶原氮端肽、层粘连蛋白与ⅳ型胶原;并测定了凝血酶原时间与血小板计数等相关指标。

Methods: Hapten of dimethoate was synthesized, then it was conjugated to bovine sera albumin and ovalbumin to prepare immunogen and coating antigen respectively by carbodiimide method.

采用生产乐果的中间体硫磷醋与l, 4-二氨基丁烷反应合成乐果半抗原,利用碳二亚胺法,将半抗原与牛血清白蛋白及卵清蛋白偶联,分别制备免疫原和包被原。

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