白膜的

Methods: Adult mice of Kunming variety were used as experiment animals. After judgement of oestrus, the oestrus period and oestrus stage were determined, and then those mouse were grouped depending on oestrus stage. The mice were dealed with diferrent dosage of PMSG and HCG to make them superovulation, afterwords were mated individually with the male mouse together at same cage. The embryoes were rinsed out and evaluated after 3.5 days when the plug had been seen. The transplant mice were born after the high-quality embryoes were transferred into the receptors mouse uterus at the same oestrus stage which was stimulated by mating with the male mouse which spermaduct had been ligated previously.

以昆明系小白鼠为实验动物，取性成熟个体，先进行发情鉴定，确定其发情周期及不同的阶段，然后按发情阶段分组，分别用不同剂量的孕马血清促性腺激素和人绒毛膜促性腺激素进行超排处理并与公鼠合笼，在见栓后的第3.5天冲胚，对所得胚胎进行鉴定；将质量较好的胚胎选出，移入同期发情并经结扎公鼠交配刺激后见栓的受体小鼠子宫内，得到移植小鼠。

Methods Hyperlipaemia and AP model were established with Triton WR1339 and Cerulein respectively. AP with hyperlipaemia model was prepared by both Triton WR1339 and cerulein simultaneously. Human albumin was used to treat the AP accompanied with hyperlipaemia. In each group, the histological score, volume of ascites, ratio of pancreatic wet/dry weigh, serum amylase and pancreatic acinar cell apoptosis in each group were compared. The level of protein kinase C membrane translocation in pancreatic tissue was detected by Western blot.

分别用Triton WR1339、Cerulein制作大鼠高脂血症和急性胰腺炎（acute pancreatitis，AP）模型，同时应用两者制作伴有高脂血症的AP模型，应用白蛋白治疗伴有高脂血症的AP，比较各组胰腺病理损害评分、腹水量、胰腺湿/干比、血清淀粉酶和胰腺组织的凋亡；Western blot检测胰腺组织PKC的膜转位。

Methods Modification I:Transverse incision was chosen on epigastirc,a piece of about 3×5 cm fascil strip with pedical as well as bilateral anterior and posterior sheath of recus and peritoneum was pulled through transverse mesocolon functioning as the supporting stick of traditional loop ostomy.The colon of ostomy was buried subcutaneously,stoma was opened instantly or secondarily depending on the patients′s condition.

一、选择上腹部正中线横向切口，将腹白线两旁的腹直肌前、后鞘连同腹膜切成3×5cm的带蒂筋膜条穿过横结肠系膜并缝合固定，替代传统袢式造瘘的支撑棒，造瘘肠段埋于皮下，根据病情需要直接或二期开放造瘘口，余手术操作基本同传统袢式造瘘术。

By using red blood cell broken-up solution,proteinase K and RNase,total DNA with high quality was extracted from the egg membranes in the incubated eggshells of two pheasant species,Reeves's Pheasant Syrmaticus reevesii and Ring-necked Pheasant Phasianus colchicus from Dongzhai Nature Reserve in Henan Province.

本实验以河南董寨自然保护区白冠长尾雉和环颈雉已孵化的卵壳为材料，利用红细胞破碎液、蛋白酶K及RNA酶等试剂，对卵壳膜内的总DNA进行了提取，建立了一种提取高质量DNA的新方法。

Finally confirmed that,The thighbone and the osteoporosis morbidity is possibly connected protein 6, respectively are: The lactoferrin light chain, membrane association protein A3, the enolase, ATP gather the enzyme, the acetyl coenzyme A reductases, the myo calcium protein; The lumbar vertebra and the osteoporosis morbidity is possibly connected protein 5, respectively are: The actin, the keratin, the enolase, ATP gather the enzyme, the myosin; The thighbone and the strong bone valuable curative effect is possibly connected protein 9, respectively are: The enolase, ATP gather the enzyme, the myo-calcium protein, the creatine activating enzyme isozyme, the phosphoglyceric acid change flavor the enzyme, the myosin, the lactoferrin light chain, the pyruvic acid activating enzyme isozyme, the crown protein; The lumbar vertebra and the strong bone valuable curative effect are possibly connected protein 8, respectively are: The carbonic anhydrase, the actin, the αB-crystal protein, 3-phospho-glycerol aldehyde oxidase, the serum albumin, ATP gather the enzyme, the myosin, the enolase.

最后确认：股骨与骨质疏松发病可能相关的蛋白6个，分别为：乳铁蛋白轻链、膜联蛋白A3、烯醇化酶、ATP合酶、乙酰辅酶A还原酶、肌钙蛋白；腰椎与骨质疏松发病可能相关的蛋白5个，分别为：肌动蛋白、角蛋白、烯醇化酶、ATP合酶、肌球蛋白；股骨与强骨宝疗效可能相关的蛋白9个，分别为：烯醇化酶、ATP合酶、肌钙蛋白、肌酸激酶同工酶、磷酸甘油酸变味酶、肌球蛋白、乳铁蛋白轻链、丙酮酸激酶同工酶、冠蛋白；腰椎与强骨宝疗效可能相关的蛋白8个，分别为：碳酸酐酶、肌动蛋白、αB-晶体蛋白、3-磷酸甘油醛脱氢酶、血清白蛋白、ATP合酶、肌球蛋白、烯醇化酶。

It shows that the average thickness of the surface layer of crystals,the palisade and cone layer and the eggshell membrane are 20.8,220.8 and 62.5μm,respectively,which accounting for 6.8%,72.6% and 20.6% of the total thickness of the eggshell.There are many vesicular holes in palisade layer with an average diameter of(0.32±0.08)μm(n=30).The function of such holes may be of significant to air exchange.The shape of eggshell pore on the surface layer of crystals is round or ellipsis.The fracture surface of the pore is funnel-shaped.

结果表明：白冠长尾雉卵壳的表面晶体层、栅栏锥体层和壳膜层的厚度分别为20.8，220.8和62.5μm，分别占卵壳总厚度的6.8%，72.6%和20.6%；栅栏层有很多蜂窝小孔，其直径为(0.32±0.08)μm(n=30)；卵壳表面有许多圆形或椭圆形的气孔，可能与胚胎发育过程中的气体交换有关，气孔横断面呈细漏斗状，在其上半部有颗粒状填充物。

This product is strong adhesion, good film-forming, antimicrobial-resistant molds, no smell, safe, reliable, easy to use, the products use a wide range of wood processing, paper processing, such as interior decoration has a good adhesion, in addition to white latex cement to increase strength and flexibility, and ceramics, foam, also has good adhesion.

本产品粘合力强，成膜性好，抗菌耐霉，无异味，安全可靠，使用方便，该产品使用范围较广，对木材加工，纸品加工，室内装修等具有良好的粘合性，此外，白乳胶可增加水泥的强度和弹性，与陶瓷，泡沫塑料，也具有较好的粘合性。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。