白细胞的

Blood were obtained for blood RT and plasma endotoxin concentration check. 6 All data underwent ANOVA analysis. Results: After 3hrs of LPS injection, 1 in hypermetabolic sepsis group, the average HR went to 334/min, the average body temperature went to 41.0℃, while in control group they were 274.9/min and 38.2℃ respectively. p=0.000, presented significant statistical differences. The average BP and breath rate in HS group were 97.1mmHg and 94.3/min , while they were 95.9mmHg and 76.4/min in control group, respectively presenting no statistical differences. 2 In HS group plasma endotoxin concentration was 0.088EU/ml while it was 0.013 EU/ml in control group presenting statistical significance, p=0.002. 3 Blood white cell ratio and total white cell counting were 82.73% and 1.90×109 in HS group, while they were 64.39% and 7.58×109 in control group presenting very significant statistical differences(p=0.001). There was no statistical significances in RBC counting and hemocrit between two groups.

结果：LPS持续灌注3h后，结果如下：1）高代谢脓毒症组的心率升高至334.0次/min、体温升高至41.0℃，而对照组则分别为274.9次/min和38.2℃，两组间有显著的统计学差异，p=0.000；血压在高代谢脓毒症组是97.1mmHg，而对照组为95.9mmHg，两组间无统计学差异；脓毒症组的呼吸加快至94.3次/min，对照组则提高至76.4次/min，两组间无统计学差异(p=0.212)；2）其血浆内毒素含量为0.088EU/ml，而对照组为0.013 EU/ml，两组间有显著的统计学差异，p=0.002；3）血中性粒白细胞比值升高至82.73%，对照组则为64.39%，两组间有显著的统计学差异（P=0.001）；白细胞总数则明显下降至1.90×109/ml，而对照组则为7.58×109/ml，p=0.001，两组间有显著的统计学差异；两组间的红细胞数及血细胞比容均无统计学差异。

RSB-66 was obtained from the SSH library of round spermatid-specific cDNAs against those of primary spermatocyte.

尽管FcαRI也是一种免疫球蛋白的Fc受体，但是它却和其它免疫球蛋白的受体有很大的不同，其它免疫球蛋白的FcRs大都定位在1号染色体的q21-23，上，而FcαRI却定位在19号染色体的q13.4，这个区域因为定位了几个包括杀伤细胞抑制受体和白细胞Ig样受体的白细胞受体家族而被称为

ObjectiveTo observe the influence on IgE、 EOS、 IL-5、 IL-12 and clinical effect of ChuanFuLingTieJi by applying on the points (Dazhui 、 Dingchuan、 Feishu、 Pishu 、 Shenshu) of children patients with asthma,and compare with the ChuanFuLing-paste, researching the mecharism of CFLTJ and providing theoretical basis for feasibility of CFL form improvement and prevention and treatment of asthma.

通过观察中药喘敷灵贴剂三伏穴位(大椎、双侧定喘、双侧肺俞、双侧脾俞、双侧肾俞)敷贴对缓解期哮喘患儿临床症状、非特异性免疫球蛋白E、嗜酸性粒细胞、白细胞介素5(IL-5)、白细胞介素12(IL-12)的影响，并与疗效确切的喘敷灵传统剂型比较，探讨中药喘敷灵贴剂穴位敷贴防治缓解期小儿哮喘的临床疗效及其可能的作用机理，从而为中药喘敷灵剂型改良的可行性及其临床推广应用提供依据。

There were location and morphous changing of megakaryocytes in all PV、ET and IMF patients;the incidence rate of JAK2V617F in PV、ETand IMF patients were 80%、26.7%、50% respectively;the mean of leucocytes in patients with JAK2V617F was higher than patiens without JAK2V617F, different stages of reticulin had no impact on the leucocytes in PV and ET patients who were dignosed in the first time.

PV、ET、IMF患者骨髓切片中均有巨核细胞的形态以及定位变化，PV， ET、IMF患者中JAK2V617F的发生率分别为80%、26.7%、50%；有突变患者的白细胞数均值高于无突变患者，初诊的PV、ET患者骨髓中不同纤维增生程度患者的白细胞均值无差别。

Meta-analysis based on included studies showed that response rate of TP regimen has no statistic significance compared with EP regimen[OR0.83,95%CI(0.63-1.090)],but myelo-suppression such as leucopenia and thrombopenia is more severe; response rate of single topotecan has no statistic significance compared with CE regimen[OR0.59,95%CI(0.22-1.60)]; response rate of TEP regimen has no statistic significance compared with EP regimen [OR1.37, 95%CI(0.82-2.28)], but myelo-suppression such as leucopenia, thrombopenia and anemia is more severe; response rate of IV topotecan has no statistic significance compared with Oral topotecan[OR0.97, 95%CI(0.60-1.57)],so as median time to progression[WMD-2.32, 95%CI(-5.72,1.09)] and median survival time[WMD-1.65, 95%CI(-7.13,3.83)],while neutropenia is more sever in IV topotecan than Oral topotecan.

分析表明，TP方案与EP方案的反应率相似[OR0.83，95％CI(0.63-1.090)]，但具有相对高的致白细胞和血小板下降的骨髓毒性；单药拓扑替康与CE方案的反应率相似[OR0.59，95％CI(0.22-1.60)]；TEP方案与EP方案的反应率相似[OR1.37，95％CI(0.82-2.28)]，TEP方案致化疗后重度白细胞下降、重度血小板下降、重度血红蛋白下降均高于EP方案；口服拓扑替康与静脉滴注拓扑替康的化疗后反应率[OR0.97，95％CI(0.60-1.57)]、中位疾病进展期[WMD-2.32，95％CI(-5.72，1.09)]、中位生存期[WMD-1.65，95％CI(-7.13，3.83)]相似，口服拓扑替康化疗后重度中性粒细胞下降明显低于静脉滴注拓扑替康。

Coli pET32/IL-11/BL(DE21 that expressed recombinant human interleukin-11 in the flask have been studied by orthogonal experiment design, including the components of culture medium (glucose, tryptone, yeast extract and pH), shaking speed, medium volume and inoculum concentration.

为了探讨发酵条件对大肠杆菌表达人白细胞介素-11融合蛋白的影响，利用正交实验设计，对影响工程菌的生长及重组人白细胞介素11融合蛋白的表达因素如培养基中的葡萄糖浓度、蛋白胨浓度、酵母提取物浓度、无机盐浓度、pH及摇床转速、装液量、接种量等在摇瓶中进行了初步的研究。

Results We found that in all individuals'blood there are an amount of leucocytes parasitized by some moving-ac-tively tiny organisms,which even were found in some of the individuals'blood platelets.After dying we had observed the extracellular fluid of leucocytes and blood platelets.Analogically,in some plant juice and tissue cells we had also found the similar tiny organism,which had an ability to survive under the heat of90℃for30minutes.

结果 观察发现所有人血液中都能找到不同百分比的白细胞内有运动活泼的微小生物寄生，部分人血小板内也有，染色后还能观察到白细胞和血小板的外浆，在部分植物的汁液和细胞中也能看到类似的微小生物，这种微小生物还能耐受90℃高温30min以上。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine, thewhole genomes of DLA-EIAV and EJAV L provirus were cloned and sequenced. Aninfectious molecular clone derived from DLA-ELAV was constructed and characterized.Three DLA-EIAV/ L chimeric viruses were constructed. Promoting efficacy of the longterminal repeat of the DLA-EIAV was charactenzed.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

To elucidate the molecular basis of the attenuation ofDLA-EIAV in virulence thus provide theory foundation for designing HIV vaccine,thewhole genomes of DLA-EIAV and EIAV L provirus were cloned and sequenced.Aninfectious molecular clone derived from DLA-EIAV was constructed and characterized.Three DLA-EIAV/L chimeric viruses were constructed.Promoting efficacy of the longterminal repeatof the DLA-EIAV was characterized.

为了揭示我国马传贫弱毒疫苗毒力致弱及免疫保护的分子机制，为人免疫缺陷病毒及其它慢病毒的免疫预防提供借鉴，我们对马传贫驴白细胞弱毒及其亲本马强毒EIAV L株前病毒进行了全基因克隆和序列测定，比较分析了二者的前病毒基因组核苷酸序列，在此基础上构建了EIAV驴白细胞弱毒株的感染性分子克隆和三株含有EIAV强/弱毒嵌合病毒基因组的重组质粒，并对EIAV弱毒株长末端重复序列的启动子活性进行了初步研究。

Methods Explore the optimal concentration of phurbol-12-myristate-acetate and calcium ionophore (A23817) inducing time, and optimal concentration of biotinyl sheep anti mouce IgG (bio-SAM-IgG) and horse radish peroxidasestreptavidin. The CD4(superscript +) cell enriched from peripheral blood mononuclear cells using solid phase anti-CDs monoclonal antibodies were induced by PMA and A23817 under influence of monensin and harvested in vary hours.

从健康人外周血单个核细胞中富集CD4细胞，加入莫能霉素，用不同浓度的佛波醇酯和钙离子载体(A23817)诱导CD4细胞不同时间，比较其胞内表达白细胞介素－2(IL-2)/白细胞介素－4(IL-4)的细胞百分率，确定最适诱生条件；用不同稀释度的IL-2McAb、IL-4McAb、不同浓度的生物素化羊抗鼠IgG(bio-SAM IgG)和辣根过氧化物酶标记的链霉亲和素作棋盘配比试验，确认McAb、bio-SAM IgG和HRP-SA的最适浓度，根据上述结果建立了检测细胞内细胞因子的BSA-ICC法。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。