白细胞溶素

Methods 40 healthy adult mice were randomly divided into control group, CTX immune-suppressed group, Fb treated group, Fb combined with CTX treated group. The influences of Fb in NK cell activity were determined by the method of NK mediated cytotoxicity test;the multiplicative capacity of lymphocyte was assayed by lymphocyte transformation rate of T cell,and the mice spleen T lymphoblast multiplication analytical method was used to measure the IL-2 level.

健康成年小鼠40只，随机分为对照组、环磷酰胺免疫抑制组、黄蘑碱溶性多糖活性组分正常小鼠给药组和Fb环磷酰胺伍用给药组，采用NK细胞介导的细胞毒试验测定自然杀伤细胞的活性，淋巴细胞转化试验测定T淋巴细胞增殖功能，鼠脾T淋巴母细胞增殖分析法测定白细胞介素2（IL-2）的活性，观察Fb对各组小鼠免疫指标的影响。

The self-made lysing solution can lyse red blood cells completely, but not destroy leukocytes and influence the expression of surface antigens on lymphocytes, monocytes and granulocytes significantly.

自制流式细胞仪专用溶血素具有很好的红细胞溶解效果，但不破坏白细胞，且对淋巴细胞、单核细胞和粒细胞群表面抗原的表达无明显影响作用。

Patients were not remission or combined with hematolysis, and there was not a donor coincided with human leukocyte antigen matching. If after Bu-CTX2 preconditioning, ciclosporin A plus methopterin schema is used for preventing graft versus host disease, many complications, low success rate or high reoccurrence rate can be detected.

受者为未缓解、合并溶血病例，且无人类白细胞抗原配型相合的血缘供者，如予Bu-CTX2方案预处理，单用环孢素A+甲氨蝶呤方案预防移植物抗宿主病，则面临并发症多、移植成功率低或移植后易复发等难题。

The selfmade lysing solution can lyse red blood cells completely ,but not destroy leukocytes and influence the expression of surface antigens on lymphocytes, monocytes and granulocytes significantly.

自制流式细胞仪专用溶血素具有很好的红细胞溶解效果，但不破坏白细胞，且对淋巴细胞、单核细胞和粒细胞群表面抗原的表达无明显影响作用。

RESULTS: Wound healing rate, wound healing time, histopathology analysis, quantity assay of macrophage, determination of hydroxyproline, proliferation of cell, assay of DNA contents and circle of cells, level of transforming growth factor-alpha, levels of interleukin-1, interleukin-6 and tumor necrosis factor, assay of keratinocyte collagenase-1, level of fibroblast growth factor receptor-1, level of monocyte chemoattractant protein-1 and level of keratinocyte plasminogen activator inhabitor type 2 were selected as the evaluation criteria of wound healing.

结果 筛选了创面愈合率、创面愈合时间、组织病理学分析、巨噬细胞定量分析、羟脯氨酸含量测定、细胞增殖情况、细胞DNA含量和细胞周期分析、转化生长因子-α水平、白细胞介素-1、白细胞介素-6和肿瘤坏死因子水平、角质细胞胶原酶-1含量测定、成纤维细胞生长因子受体-1水平、单核细胞化学诱导蛋白-1水平和角质细胞纤溶酶原活化抑制剂-2水平等十三种创面愈合评价指标。

Objective:To investigate the effects of musk-1, a glucoprotein component isolated from the water extract of musk, on some functions of rat polymorphonuclear leukocytes activated by IL-8 in vitro.

目的：观察麝香糖蛋白成分麝香-1对白细胞介素-8(IL-8)激活的大鼠中性白细胞某些功能，包括超氧阴离子产生和溶酶体酶释放的影响。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。