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白细胞

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Objective To study the relationship between cytokine and development of Graves' disease through measurement of their level changes during antithyroid drug treatment.

目的:测定格雷夫斯病患者血清肿瘤坏死因子-α、白细胞介素-1、白细胞介素-2、白细胞介素-6水平及在抗甲状腺药物治疗过程中的变化,探讨其与格雷夫斯病发生发展之间的联系。

In 6 patients with chronic myeloid leukemia, all of them presented very high white blood cell counts (〉45 × 10^9/L) and represented completely wider and larger unimodal distribution of leukocyte histogram (100- 450 fl), among them 83.3 % cases were extremely higher (〉 100 × 10^9/L).

慢性粒细胞白血病患者白细胞总数均〉45 10^9/L,其中83.3%的患者白细胞总数异常增高(〉100 10^9/L),白细胞直方图均呈形状单一而分布广泛(100-450fl)的大细胞峰图形。

Methods Serum contents of hyaluronic,type IV collagen,cholalic acid,tumor necrosis factor,transforming growth Factor-α,interleukin-1(IL-1),interleukin-6(IL-6)and interleukin-8(IL-8)were detected by radioimmunoassay and were analyzed.

方法应用放射免疫法对患者血清透明质酸、IV型胶原、胆酸、肿瘤坏死因子、转化生长因子-α、白细胞介素-1(IL-1)、白细胞介素-6(IL-6)和白细胞介素-8(IL-8)进行测定和分析。

Results Erythrocyte count, hemoglobin concentration and hematocrit of all the patients with malignant digestive tumor decreased, mean corpuscular volume and mean corpuscular hemoglobin increased, arid there was inconspicuous change in total leukocyte count. The indications of megaloblastic anemia and proliferative anemia in bone marrow hemogram were found in the patients. It was common that the percentage of neutrophils was higher and that of lymphocytes was lower than that of the normal controls.

结果 消化道恶性肿瘤患者的红细胞计数、血红蛋白、红细胞压积均减少,平均红细胞体积、平均红细胞血红蛋白量增高,均表现为巨幼细胞性贫血,骨髓象呈增生性贫血改变,而白细胞总数的变化较小,白细胞分类,中性粒细胞百分比超过正常范围与淋巴细胞百分比低于正常范围者多见,血小板数异常远较血红蛋白与白细胞为少,但36例肝癌患者中28例出现血小板减少。

Nodosus Pilin gene, the ovine IL-2 gene with synthesized primers with EcoR1 and BamH1 recognition sequence at 5'and 3'end respectively was amplified and cloned from the peripheral blood of ovine.

从健康绵羊血中分离白细胞,用TRIZOL试剂盒提取绵羊白细胞RNA,以RNA为模板,用设计OviIL-2的引物进行RT-PCR和PCR,扩增出设计有BamHl和EcoRl限制性酶切位点的绵羊白细胞介素2。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8,酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2(IL-2)和Th2型细胞因子白细胞介素-4(IL-4)、白细胞介素-5(IL-5)水平,逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达;ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

Further,with indirect immunohistochemistry technique,CD25(IL-2 receptor or IL-2R) cross-reaction has been identified on lymphocyte membrane of Ctenophaxyngodon idellus and Trionyx sinensis using mouse monoclonal antibody directed against Human Leucocyte Differentiation Antigen CD25(IL-2R).

进一步用抗人白细胞分化抗原CD25(IL-2受体,简称IL-2R)单克隆抗体进行免疫组化交叉反应提示草鱼、中华鳖淋巴细胞膜上含有与人类IL-2R类似功能和结构的物质。关键词:白细胞介素-2;白细胞介素-2受体;发育和比较免疫学;草鱼;中华鳖

Unfortunately, practical methods of safely and effectively transfusing adequate numbers of granulocytes or other white cells are not yet available to prevent infection that occurs as a result of low white cell count.

遗憾的是,安全有效地输入适量的粒性白细胞或其它白细胞尚不能防止感染,这样就会出现白细胞数量的减少。

objective to study the effects of berbamine and batiolun on ldh metabolizability in the process of increasing leukocyte.methods enzyme biochemical techniques is used to study the effect of berbamine and batiolˉun in the process of inceasing leukocyte and also to study the effect of ldh metabolizability.results when leukocyte is inhibited by the immune inhibitor cycolophosphamcide,berbamine and batiolun are compared of the effect of ldh activity.conclusionthe study draws the conclusion that both berbamine and batiolun can increase thegranule cell,and they also have some effect of the ldh metabolizability.

目的 研究小檗胺和鲨肝醇升高白细胞作用及对细胞内ldh的代谢的影响。方法采用酶细胞化学方法,对两种药物在治疗由免疫抑制剂环磷酰胺所致的白细胞减少症时,对乳酸脱氢酶活性的影响进行了比较观察。结果小檗胺和鲨肝醇均可升高粒细胞,同时对细胞内ldh的代谢有一定的影响。结论小檗胺和鲨肝醇能使外周血粒细胞增加,可用于治疗多种因素引起的白细胞减少症。

Blood samples were collected from mice in each group and the hematological parameters were detected at the end of the experiment.RBC,HGB,HCT,MCV,MCH and MCHC of the mice in the model group were significantly lower than those in the control group.Compared with the model group,RBC,HGB,HCT,MCV,MCH and MCHC were increased significantly,but WBC,Lym and Mid were reduced significantly in the experimental Group 3 administered with high dose of polysaccharides solution.

结果显示,模型组小鼠的血液指标红细胞数、血红蛋白浓度、血细胞比容、平均红细胞体积、平均红细胞血红蛋白含量、平均红细胞血色素浓度显著低于对照组的相应指标;与模型组相比,给予高剂量姬松茸水溶性粗多糖的试验组3的血液指标RBC、HGB、HCT、MCV、MCH、MCHC均显著提高,而白细胞数、小型白细胞数和中型白细胞数则显著下降。

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