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PKC and PKA activity assays showed that 0.03μmol/L of 20-HETE increased PKC and PKA activity in isolated cardiomyocytes, while chelerythrine (5μmol/L) and H-89 (5μmol/L) inhibited these effects.

PKC和PKA活性测定的结果表明20-HETE(0.03μmol/L)增加心肌细胞PKC和PKA的活性,并且白屈菜赤碱(5μmol/L)和H-89(5μmol/L)抑制该效应。

PKC and mitochondrial KATP channels were inhibited with chelerythrine and 5-hydroxydecanoate pretreatment, respectively.

试验中使用白屈菜红碱、5-羟基癸酸分别抑制心肌蛋白激酶 c 、线粒体 ATP 钾离子通道。

Chelerythrine could inhibit the glucosyltransferase and extra-cellular synthesis of water-insoluble glucan of Streptococcus mutans.

白屈菜红碱对变形链球菌葡糖基转移酶和细胞外水不溶性多糖的合成具有显著抑制作用。

METHODS: NE has been shown to be a potent stimulator for neonatal rat ventricular myocyte hypertrophy in vitro. We used the activator of PPAR γ, the inhibitor of protein kinase C chelerythrine to influence protein content.3H?

在培养新生大鼠心肌细胞中,采用RSG,PKC的激动剂佛波醇酯,NE和PKC的阻断剂白屈菜季氨碱观察罗格列酮在NE和PMA诱导心肌肥大中对PKC活性和c?

MethodsNormal human mesangial cells were divided into 4 groups: a control group(N, 5 mmol/L glucose), a high glucose group (H, 30 mmol/L glucose), a PKC inhibition group (P, 30 mmol/L glucose plus 10-5mol/L chelerythrine chloride), and an mannitol group (M, 5 mmol/L glucose plus 25 mmol/L mannitol).

将NHMC分4组: N组(对照组,5 mmol/L葡萄糖);H组(高糖组,30 mmol/L葡萄糖);P组(抑制剂组,30 mmol/L葡萄糖+10-5mol/L白屈菜红碱);M组(甘露醇组,5 mmol/L葡萄糖+25 mmol/L甘露醇)。

Chelerythrine and 5-hydroxydecanoate abolished cardioprotection in both SEVO and EtOH cardioprotected hearts.

白屈菜红碱、5-羟基癸酸在 EtOH 组和 SEVO 组中均能减弱原本的心肌保护作用。

Reactive oxygen species scavenger N-2-mercaptopropionyl glycine, protein kinase C antagonist chelerythrine, and mitochondrial KATP channel closer 5-hydroxydecanoate each blocked protection from 2 minutes of acidosis as they did for postconditioning.

活性氧簇清道夫N-2硫丙酰甘氨酸、蛋白激酶C拮抗剂白屈菜红碱、线粒体KATP通道关闭剂5-hydroxydecanoate都可以阻断两分钟的酸中毒和后条件的保护作用。

METHODS: The Chelerythrine was used as the experimental group with concentrations ranging from 24.4μg/ml to 390.6μg/ml prepared with BHI broth medium with contained 2% glucose, and BHI culture medium was used as the control group.

采用二倍稀释法,用含2%蔗糖的脑心浸萃液肉汤琼脂液体培养基,将白屈菜红碱稀释为从390.6μg/ml到24。

The 20-HETE-induced increases in [Ca2+]i, whole-cell L-type Ca2+ channel currents and the amplitudes of Ca2+ transients were blocked by extracellular application of chelerythrine (an inhibitor of protein kinase C, PKC) and H-89 (an inhibitor of cAMP-dependent protein kinase, PKA).

上述20-HETE诱导的[Ca2+]i、全细胞L-型钙离子通道电流、钙瞬变幅度的增加等效应,可被蛋白激酶C抑制剂白屈菜红碱和cAMP依赖蛋白激酶抑制剂H-89阻滞。

PKC inhibition by chelerythrine enhanced both LPA-and CaCl2-induced ICl.

白屈菜红碱通过抑制 PKC 而增强 LPA-和 CaCl2-介导的 IC1。

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