白喉

We are injected with diphtheria antitoxin produced in horses because the antitoxin manufactured by our bodies may not be enough to prevent diphtheria.

我们被注射从马身上制造出来的白喉抗毒素，因为我们体内的抗毒素可能不足以抵御白喉。

We are injected with diphtheria antitoxin produced in horses beause the antitoxin manufactured by our bodies may not be enough to prevent diphtheria.

我们注射从马身上制造出来的白喉抗毒素，因为我们体内的抗毒素可能不足以抵抗白喉。

Diphtheria is treated with an antitoxin that neutralizes the toxin and produces long-term immunity.

治疗方式为及时注射白喉抗毒素，产生长期的免疫力。白喉抗毒素是异性蛋白。

Diphtheria toxin is an exotoxin secreted by Corynebacterium diphtheriae that has been lysogenized by β bacteriophage that carries the DT gene.

白喉毒素是棒状白喉杆菌被β噬菌体感染后分泌的一种外毒素。

The principal purpose of this study is to set up the isolation methods for diphtheria toxin expressed by genes, to purify the toxin with complete biological activities, and to make researches on its toxicities. In the experiments, the target gene was inserted into the cloning vectors--pET22b, then the vectors were introduced into host cells -BL21(DE3). The expression of the toxin was induced by addition of IPTG. The expression products were purified by His-6 label affinity chromatography. Then the diphtheria toxin expressed was transferred to PVC membrane using semidry blotter to determine the amino acid sequence of N-terminal. The "nicked" diphtheria toxin linked by disulfide bond was gained by protease hydrolysis.

为建立重组基因表达白喉毒素的分离纯化方法，制备出具有生物活性的蛋白质毒素，构建了白喉毒素表达载体pET22b-DT，并转入大肠杆菌BL21(DE3)中诱导表达，以融合表达的His-6作为标签通过亲和层析纯化；表达毒素经蛋白酶酶切后，形成了以二硫键连接的与天然结构相同的带"缺口"的白喉毒素，并测定白喉毒素对豚鼠的急性毒性和细胞毒性。

Vaccinating diphtheria toxoid vaccine is one of the comprehensive measures of diphtheria prevention. The diphtheria incidence is controlled effectively in Binzhou city.

白喉类毒素疫苗预防接种是白喉综合防制的主要措施之一，滨州市人群白喉发病基本得到控制。

Lesser Whitethroat: nominate plus blythi , though I read that blythi may be synonymous with curruca anyway.

白喉林莺 Lesser Whithroat ：指名亚种加上 blythi 亚种，虽然我读到 blythi 亚种可能就是白喉林莺的同义词（编按：意思是指名亚种和 blythi 亚种是同一个亚种？）。

According to the results of quantum chemistry calculation and the present research status in the relationship between the structures and the functions of DT, the El 54 in DT catalyzing domain was mutated to aspartic acid and arginine in order to study the effects of the alteration on the biological activities.

在量子化学计算的基础上，结合目前关于白喉毒素结构与功能的研究状况，选择把白喉毒素催化区的第154位谷氨酸分别突变为天冬氨酸和精氨酸，研究此处电荷性质的改变对生物活性的影响。

Diphtheria is a contagious, sometimes fatal, infection of the upper respiratory tract caused by the bacterium Corynebacterium diphtheriae.

白喉是由白喉杆菌引起上呼吸道感染的，有时会致命的一种传染性疾病。

A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

结果：1、用PCR方法检测A组链球菌：以A组链球菌致热性外毒素基因speB为靶序列，设计的扩增引物对全部对照菌株的扩增结果为阴性，而全部A组链球菌参考株均能扩增出特异的345bp片段，其中包括三株猩红热链球菌，检测敏感性为6.5pg／μl DNA.2、用PCR方法检测白喉杆菌：以白喉外毒素基因toxB为靶序列，设计的扩增引物对全部白喉杆菌参考株均能扩增出特异的318bp片段，而全部对照株的扩增结果为阴性，检测敏感性为850fg／μl DNA.3、用PCR方法检测嗜肺军团菌：以嗜肺军团菌巨噬细胞感染增强子基因mip为靶基因，设计的引物对嗜肺军团菌14个血清型参考株均扩增出特异的340bp片段，而鉴别对照株包括三株非嗜肺军团菌均未扩增出任何片段。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。