瘤胃的

In two series experiments by-pass nitrogen, amino acid passage to the abomasum, rureen metabolic parameters and nitrogen utilization were studied respectively.

利用四头装有瘤胃和皱胃瘘管的阉公羊以及四头未装瘘管的阉公羊进行两系列实验，测定通过瘤胃蛋白、皱胃氨基酸通过量以及瘤胃消化代谢和氮的利用。

Joins the rumen buffer, the ph conditioner, adjustment rumen ph and the osmotic pressure, maintains the ideal rumen acidic environment, may enhance the milk cattle and sheep immunity, the enhancement physique and so on, reduces the afterbirth not, ketosis, mastitis and so on, prevents puerperal fever and the rumen acidosis, increases the service life and the reproduction rate.

加入瘤胃缓冲剂、 ph 调节剂、调节瘤胃 ph 和渗透压，维持理想的瘤胃酸性环境，可提高奶牛羊免疫力，增强体质等，减少胎衣不下、酮病、乳腺炎等，预防产褥热和瘤胃酸中毒，增加使用年限和繁殖率。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Experiments were done on four dairy goats fitted with rumen cannulae and fed under different feeding regimes to measure the variations of ciliate protozoal population, pH and Eh in the rumen.

应用4头置有永久性瘤胃瘘管的乳用山羊，进行了三种不同饲养制度下瘤胃纤毛虫种群及pH和Eh变化的测定。结果表明，日粮性质是影响山羊瘤胃纤毛虫种群变化的主要因素。纤毛虫密度以含饲期喂精料加干草最高；精料加青草次之；放牧青草期最低。

These results demonstrate that BSN2 can enhance some ruminal bacteria and protozoa growth as well as ruminal fermentation, thus improving milk production, and milk component production.

说明BSN2具有促进瘤胃某些瘤胃微生物种类生长的作用，促进瘤胃发酵，从而改善奶牛奶产量和乳成分产量。

Three in vitro gas production experiments were conducted using the procedure described by Menke et al.(1979) to investigate the effect of steam conditioning time, processing method and corn variety on the gelatinization of starch and ruminal fermentation characteristics of corn grains.

本文应用人工瘤胃法，进行以下三个试验研究：1 不同调制时间预处理的蒸汽压片高油玉米对淀粉糊化度和人工瘤胃发酵的影响；2 比较最佳调制时间处理的蒸汽压片、微化、膨化及爆破不同方法处理的高油玉米对淀粉糊化度和人工瘤胃发酵的影响；3 不同品种玉米对淀粉糊化度和人工瘤胃发酵的影响。

By developing the optional measurement condition for ruminal fermentation products and comparing the effects of different levels of tannic acid on fermentation and methane production, the proper adding levels of tannic acid in vitro was established. We also examined the characteristics of rumen fluid fermentation and methane production of tannic acid unsupplemented or supplemented with different forage : concentrate ratios by using gas production method.

通过对瘤胃体外发酵系统中单宁酸的生物降解以及对瘤胃内环境影响的研究，初步确立了单宁酸改善瘤胃发酵的适宜添加量及其效果；并探讨了在不同精粗比底物条件下单宁酸对瘤胃体外发酵和甲烷产量的作用。

The effects of polyunsaturated fatty acid from soybean oil and cottenseed oil on the accumulation of Trans vaccenic acid -biohydrogenation of ruminal microbes and rumen fermentation were researched with artificial rumen.

采用人工瘤胃法研究了豆油和棉籽油中亚油酸等多聚不饱和脂肪酸在瘤胃微生物作用下的氢化中间产物trans11油酸变化规律以及对瘤胃发酵的影响。

Experiment(2): Six one years old gansu high mountain fine wethers(avg wt 25~30 kg) fitted with permanent ruminal and proximal duodenal cannulae were assigned to double 3×3 Latin square design, to measure the apparent digestibilities of nutrients in rumen, postrumen and total tract and the apparent nitrogen retention as well as the dynamic changes of the rumen metabolic parameters in wethers fed with diets which SC:NSC ratio were respectively 1.57, 1.95 and 2.29.

试验：选用6 只1 岁左右，体重25~30kg，装有永久瘤胃瘘管和十二指肠近端套管的甘肃高山细毛羯羊，采用3×3 有重复拉丁方设计，研究了SC： NSC 比例(1.57、1.95、2.29)饲粮的瘤胃内、瘤胃后和全消化道营养物质表观消化率及氮表观存留率，以及瘤胃内环境参数的动态变化。

Four sheep with ruminal and duodenal cannulas were used in a 4×4 Latin square design to study the effects of rumen protected fat on the function of rumen metabolize.

选择体重相近并安装有瘤胃瘘管和十二指肠瘘管的小尾寒羊4只，采用4×4拉丁方实验设计方法，研究了脂肪酸钙、植物脂肪粉和硬脂肪对绵羊瘤胃PH、氨氮浓度、采食量、营养物质消化率、血液生化指标以及瘤胃营养物质降解率的影响。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。