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The expression absence of LRRC4 was ascribed to the loss of homozygosity of 7q32-ter in U251 cell lines. Conclusion The expression of LRRC4 gene is absent in glioblastoma cell lines, and it offers the important experiment proof for LRRC4 to act as a new candidate of brain tumor suppressor gene from glioma.

通过对胶质瘤细胞系基因组DNA中LRRC4的ORF序列的PCR扩增和测序分析,发现SF126,SF767,M17细胞中,ORF序列第279位氨基酸的第3个密码子存在同义点突变(3/5),而U251和U87细胞基因组DNA中发生了LRRC4基因的缺失突变(2/5)。

Results In all patients,36 patients had single lump,15 with nodular,and 10 with thyriod daenoma.Fourty one patients lumps were low heterogeneous echo,30 patients had calcification,10 patients had liquescence.

结果 本组病例中单发36例合并结节性甲状腺肿15例,合并腺瘤10例,肿瘤呈不均匀低回声41例,内显示微小钙化点30例,出现液化10例,显示血流丰富19例,难以显示血流信号5例。

And then adding NAA at low concentration in MS was used to give rise to roots. We also found that lower level of hormone could control effectively browninng and vitrifaction during the culture and G1 n (6mg/L) and AgNOj (2mg/L) supplemented in shoot induction media could improve the shoot information rates apparently (about90%).The whole period of plant regeneration from leaflets of peanut could be divided as five steps: germination - shoot induction -shoot elongation-rooting- tranplant.

用1/2MS培养基萌发花生种子,9-10d后,从无菌花生苗上切取幼嫩叶片中部为外植体。2500Lux光照和27±1℃条件下,在诱芽培养基(MS+BA3mg/L+NAA0.8mg/L+AgNO_32mg/L十Gln 6mg/L)培养12-14d即可观察到明显芽点或瘤状突起,较前人报道的培养时间大大缩短了,4w后芽点进一步发育成丛生芽,芽诱导率达90.2%,每个外植体平均产9个丛尘芽,然后转至培养基MS+BA3 mg/L+AgNO_32 mg/L上诱导芽的伸长,3-4w后可长至3-4cm,切下带有2-3片叶片的幼芽移至生根培养基(MS+NAA0.8mg/L+AgNO_32mg/L),1w后切口处可见白色不定根形成。

Objective:To study the effects of mag netic material which has low curie temperatue induced by high frequency electromagnet for tumors.Methods:The needle-like magnetic material made of nickel-copper alloy which has low cuir e temperature,was implanted in S180 tumors of mice.

将镍铜合金制作的居里点温度为60 ℃的柱状磁性材料植入小鼠S180 内,从体外用高频电磁场诱导植入瘤体内的磁性材料感应产热,观察瘤体的温度变化和加热后的病理改变、瘤体大小及动物生存时间等。

Methods: The needle-like magnetic material made of nickel-copper alloy which has low cuir e temperature,was implanted in S180 tumors of mice.Then high frequency el ectrom agnetic field was applied outside body to make the magnetic material produce the hyperthermia.

将镍铜合金制作的居里点温度为60 ℃的柱状磁性材料植入小鼠S180 内,从体外用高频电磁场诱导植入瘤体内的磁性材料感应产热,观察瘤体的温度变化和加热后的病理改变、瘤体大小及动物生存时间等。

Methods: 45 cases with sphenoidal ridge meningioma operated were retrospectively analyzed. Acording to the site,all the 45 cases were divided into 2 groups ,outer(32/45) and inner(13/45) sphenoidal ridge meningioma . All the cases were operated with microscope.

回顾分析一组45例蝶骨嵴脑膜瘤手术治疗的资料,所有病例根据其生长部位,分为内侧型和外侧型两种,其中外侧型32例,内侧型13例,均采用翼点入路或改良翼点入路,显微镜直视下行手术切除。

The sulfamate moiety in the structure of topiramate is chemically related to known CA inhibitors, and in fact, topiramate inhibits CA activity. To further explore the pharmacological action of topiramate, and clarify the possibility of anti-tumor growth and metastasis of CA inhibitors and its relation with AQP1, we studied the effect of topiramate on adhesion of high metastatic prostate carcinoma to laminin, and established the spontaneous metastatic model of Lewis lung carcinoma to studied the influences of topiramate on tumor growth and metastasis and AQP1 expression in lungs and primary tumors of mice.

为了深入研究托吡酯的药理作用,阐明CA抑制剂和水通道蛋白AQP1相互作用影响肿瘤生长和转移的可能性及其机制,我们研究了托吡酯对高转移的前列腺癌细胞粘附于基质成分层粘连蛋白的影响;以Lewis肺癌自发性转移为模型,考察了托吡酯对肿瘤生长和转移的影响;同时观察了托吡酯对荷瘤小鼠肺组织及原发瘤组织AQP1表达的影响;检测了其对血管新生的作用;利用体外AQP1表达模型,研究了CA抑制剂对AQP1功能的影响;此外,用蛋白质组学的关键技术,通过对模型组与托吡酯治疗组血清蛋白质组的比较分析,来寻找与肿瘤转移相关的蛋白质,探索托吡酯的可能作用靶点。

Methods: The microanatomy of these interspaces was studied in 8 sides of human cadaver specimen with sphenoid approach. Seven cases of pituitaries and four craniopharyngiomas were diagnosed by MRI, which were removed bit by bit through the interspace superior to the fork of internal carotid artery and the first to the fourth interspaces in skull base.

在8侧尸体头上采用翼点入路观察颈内动脉分叉上间隙的显微结构;并对磁共振诊断为大型垂体瘤7例及颅咽管瘤4例者,采取翼点入路、联合应用颈内动脉分叉上间隙及颅底第1~4间隙,行瘤内减压、逐步切除肿瘤。

LRIG3 could be a targeted protein for gene therapy of glioma.

LRIG3可通过EGFR信号系统影响胶质瘤细胞的生长,有望成为胶质瘤分子治疗的靶点。

For group H2, two thermoseeds with 70℃ curie point were implanted into tumor tissues of each rat with a heating time of 6 minutes. Histological and immunohistochemistry observations on tumors were carried out in groups H1, H2 and C immediately and in 24 hours after the heating treatment.

将接种Walker-256肿瘤细胞的Wistar大鼠随机分为5组,C组、M组、T组、H组(加温治疗H1组,H2组),H1组:瘤内植入2颗居里点57℃热籽,持续30 min;H2组:2颗居里点70℃热籽,持续6 min。

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