瘤

Major features include facial angiofibromas, shagreen patches, ash-leaf spots, subependymal nodules, subependymal giant cell astrocytoma, cardiac rhabdomyoma, renal angiomyolipoma, lymphangioleiomyomatosis, retinal hamartoma, and macules.

主要特征包括面部血管纤维瘤，鲨革斑、烟叶状白斑、室管膜下结节、室管膜下巨细胞星形细胞瘤、心脏横纹肌瘤、肾平滑肌血管脂肪瘤、淋巴管肌瘤病，视网膜错构瘤和斑疹。

The other cancers with shorter follow up had three years overall and event free survival as follow: 75% and 62% for Medulloblastoma, 74% and 67% for Non-Hodgkin Lymphoma, 100% and 91% for extracranial Germ Cell Tumour, 30% and 20% for advanced neuroblastoma, 68% and 52% for Ewing's Sarcoma, 87% and 79% for Wilms' Tumour, 82% and 80% for Osteosarcoma, 56% and 45% for Rhabdomyosarcoma, and 60% and 50% for Hepatoblastoma respectively.

其他癌症经三年的短期随访，总存活率及无并发症存活率分别是：成神经管细胞瘤为 75%及 62%；非何杰金氏淋巴瘤为 74%及 67%；颅外的胚细胞瘤为 100%及 91%；已播散成神经细胞瘤为 30%及 20%；尤因氏肉瘤为 68%及 52%；威尔姆斯氏瘤为 87%及 79%；骨肉瘤为 82%及80%；横纹肌肉瘤为 56%及 45%；肝母细胞瘤为 60%及 50%。

Results In 13 cases,8 cases were germinoma,1 case was teratoma,1 case was pinealocytoma,2 cases were gliomas,1 case was meningioma.

结果 本文13例中，生殖细胞瘤8例，畸胎瘤1例，松果体细胞瘤1例，胶质瘤2例，脑膜瘤1例。

In this study, the role of newcastle disease virus combined thermic solidified tumor vaccine in inhibiting growth of tumor and immune control was investigated, and rate of inhibiting tumor and cellular immunity were measured.

为探讨新城疫病毒联合热固化瘤苗对小鼠肿瘤的抑瘤作用和免疫调节作用，实验检测了荷瘤小鼠的抑瘤率和细胞免疫功能。实验将接种上SP2/0及H22瘤细胞的荷瘤小鼠分为单纯NDV治疗组和NDV+热固化瘤苗治疗组。

The results showed that (1) the positive staining of ChAT was obviously observed in the cells of the three kinds of human pituitary adenoma, however, it was lower than that in normal human pituitary gland;(2) ACh had a similar effect on the proliferation of the three kinds of human pituitary adenoma cells. ACh at 0.1～10 μmol/L decreased the ［3H］TdR incorporation and the MTT A value in a dose-dependent manner. At the same time, ACh decreased the ratio of S or G2 phase pituitary adenoma cells significantly, but increased the ratio of G1 phase pituitary tumour cells markedly;(3) the effect of acetylcholine on the proliferation of human pituitary adenoma cells was inhibited by atropine, but not by tubocurarine;(4) ACh had no effect on the apoptosis of human pituitary adenoma cells cultured in vitro.

结果发现：(1)三种垂体腺瘤细胞中均有胆碱酯酶的表达，但明显少于正常垂体；(2)ACh对三种类型的人垂体腺瘤细胞增殖代谢的影响相类似，不同浓度的ACh能明显抑制体外培养的三种人垂体腺瘤细胞的增殖，呈明显的剂量效应关系，同时ACh能减少垂体腺瘤细胞进入S、G2期的细胞比例，而使处于G1期的细胞比例增加；(3)ACh的这种作用可被阿托品阻断，但不受筒箭毒的影响；(4)ACh对体外培养的三种人垂体腺瘤细胞凋亡无明显影响。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

None of the GISTs, desmoid tumors, inflammatory myofibroblastic tumors, schwannomas, dedifferentiated liposarcomas, or malignant peripheral nerve sheath tumors showed cytoplasmic reactivity for smoothelin.

所有GISTs、韧带样纤维瘤、炎性肌纤维母细胞瘤、神经鞘瘤、去分化脂肪肉瘤和恶性外周神经鞘膜瘤病例的瘤细胞浆均不表达smoothelin。

Results Ependymoma and astrocytoma are the most common spinal cord gliomas.The rate of radical resection was higher in ependymoma than in astrocytoma cases.

结果脊髓胶质瘤以室管膜瘤和星形细胞瘤为主，其中以室管膜瘤居多而且手术全切率较高，星形细胞瘤手术全切率较低。

Results The study population consisted of 11 schwannomas,7 neurofibromas,1 ganglioneuroma,1neurofibromatosis,4 malignant schwannomas and 2 neurofibrosarcomas.

结果 神经鞘瘤 1 1例，神经纤维瘤 7例，节细胞神经瘤 1例，神经纤维瘤病 1例，恶性神经鞘瘤 4例和神经纤维肉瘤 2例。

All of these patients underwent plain and contrast-enhancement CT scan. Results: Thirteen cases included 5 neurilemmomas (3 malignant cases included), 5 neurofibromas, 1 neuroblastoma, 1 ganglioneuroma, 1 malignant paraganglioma. In the 13 cases, 2 cases derived from plexus sacralis, 2 cases located in the pelvic peritoneal space, 6 cases located in the pelvic extrapentoneal space, and 3 cases located in the vesica extraperitoneal space.

结果：神经鞘瘤5例（其中恶性3例），神经纤维瘤5例，神经母细胞瘤1例，节细胞性神经瘤1例，恶性副节细胞瘤1例。2例起源于骶丛，经由骶孔向盆腔内生长；2例起源于盆腔，其中1例源于膀胱，1例位于膀胱直肠陷窝；直肠腹膜外间隙6例；膀胱腹膜外间隙3例。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了