瘤

The pcDNA〓-apoE〓 plasmid was transfected to SK-N-SH Neuroblastoma cell by liposome. It could be screened by G〓. The immunohistochemical assay was shown that neuroblastoma cell transfected by pcDNA〓-apoE〓 expressed the recombinant apoE protein. The intracellular expressed recombinant protein could inhibit the death of neuroblastoma cell induced by beta amyloidal peptides 25-35 fragment.

用pcDNA〓—apoE〓真核表达载体与脂质体共转染神经成纤维胶质瘤细胞，用G〓选择压力筛选，细胞免疫荧光检测表明，pcDNA〓—apoE〓转染的神经成纤维胶质瘤细胞表达了apoE〓重组蛋白，这种内源性的apoE〓重组蛋白对25μM Aβ25—35多肽诱导的神经成纤维胶质瘤细胞的死亡具有拮抗作用。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Methods MR Perfusion Weighted images was performed in 47 patients with meningiomas followed by conventional imaging.Results The mean rCBV values of angioblastic was the highest in the parenchyma of tumor.

对47例脑膜瘤病人行MR灌注成像检查，对5种亚型的良性脑膜瘤的MR灌注成像进行分析，并与非典型性及恶性脑膜瘤进行比较。

In 14 patients with angiogenic tumors of the soft tissue ( 13 benign tumors and 1 malignant tumor) were analyzed. Results Benign hemangiomas included cavernous (n=7), intramuscular (n=2), mixed (n=1), racemosum (n=1), glomus (n=1) and angiomatosis (n=1). The vascular component within the tumor displayed iso-intensity on T1WI relative to the skeletal muscle, and hyper-intensity on T2WI. One case of hemangiopericytoma showed slightly low signal on T1WI and slightly high signal on T2WI.

结果 13例良性肿瘤中，海绵状血管瘤7例，肌肉内血管瘤2例，混合型血管瘤、蔓状血管瘤、血管球瘤、血管瘤病各1例，瘤血管成分在T1WI上呈等信号，T2WI上呈高信号，肿瘤有不等量的纤维组织和脂肪组织；血管外皮细胞瘤1例，T1WI上呈略低信号，T2WI上呈略高信号。

In addition, the clinical data of 96 FAP cases clinically diagnosed between 1985 and 2000 were analyzed retrospectively, which included clinical (enteral and apenteric manifestations, methods of diagnosis and screening, surgical modality and follow-up findings).

我们认为结肠镜是FAP最可靠、最有效的临床诊断方法并在家系成员筛选中可发挥重要作用；CHRPE是具有高度特异性和敏感性的辅助诊断方式，可作为对未成年家系成员筛选的首选方法；FAP常伴发上消化道病变包括胃窦部增生性息肉，十二指肠腺瘤发病率高，但癌变少见；腹腔硬纤维瘤是FAP的严重结肠外病变，术后复发率高；FAP术后如直肠粘膜有残留，其腺瘤复发及癌变危险性高，应定期结肠镜随访。

But injury of normal tissue is one of important factors which have to stop killing tumor cell thouoghly, in all process of tunor therapy Since the injury all over argans and cells of the body by free radical, especially significant side-effect to heart via ariblastine ,is universally accepted, we chose Adriamycin as chemical therapy medicine in our study.

由于阿霉素具有比较公认的自由基周身性损伤、特别是对心脏损伤的突出副作用，因此，本课题选择阿毒素作为化疗药物，模型制作采用在SD大鼠肝表面种植Walker－256瘤细胞株，于种瘤后第11天按2mg~(-1)kg体质量给单纯化疗组和化疗保护组的大鼠腹腔注射阿霉素，正常对照组和荷瘤对照组按相同比例腹腔注射生理盐水；同时，每日上午按0.5ml/100g体质量经胃给于化疗保护组抗氧化剂混合液，其余各组每日给于相同剂量的三蒸水；下午按0.1ml/100g体质量经胃给于化疗保护组维生素E溶液，其余各组每日给于相同剂量的处理过的油。

But injury of normal tissue is one of important factors which have to stop killing tumor cell thouoghly, in all process of tunor therapySince the injury all over argans and cells of the body by free radical, especially significant side-effect to heart via ariblastine ,is universally accepted, we chose Adriamycin as chemical therapy medicine in our study.

由于阿霉素具有比较公认的自由基周身性损伤、特别是对心脏损伤的突出副作用，因此，本课题选择阿毒素作为化疗药物，模型制作采用在SD大鼠肝表面种植Walker－256瘤细胞株，于种瘤后第11天按2mg~(-1)kg体质量给单纯化疗组和化疗保护组的大鼠腹腔注射阿霉素，正常对照组和荷瘤对照组按相同比例腹腔注射生理盐水；同时，每日上午按0.5ml/100g体质量经胃给于化疗保护组抗氧化剂混合液，其余各组每日给于相同剂量的三蒸水；下午按0.1ml/100g体质量经胃给于化疗保护组维生素E溶液，其余各组每日给于相同剂量的处理过的油。

This result indicates that WT1 gene plays an important role in differentiation and development of fetal kidney and may be the factor that promotes metanephric blastemal cell to differentiate into epithelial cell.

结果显示小胎龄肾组织中WT1蛋白在胚基细胞和幼稚肾小球细胞核表达而大胎龄组肾组织中WT1在肾小管细胞胞浆表达，阳性率分别为57.1％(8/14)和46.2%(6/13)，提示WT1基因在胚胎肾分化发育的过程中起着重要作用，WT1蛋白可能是促进后肾胚基细胞向上皮细胞分化的调控因子，其表达在时间上和空间上都受到严格的调控，WT1的表达异常可能导致胚基细胞分化停滞。17例肾母细胞瘤WT1蛋白表达阳性率为41.2%(7/17)，阳性部位在胚基型和上皮型肿瘤细胞核，表达部位和阳性率与早期胚胎肾相似，其中间质型肾母细胞瘤均为阴性，胚基型和上皮型肾母细胞瘤阳性率70%(7/10)，两组间阳性率有显著差异。

But a few cases, compared with benign, such as muscle blastoma, chondroblastoma and osteoblastoma.

但少数情况则为良性，如肌母细胞瘤、软骨母细胞瘤和骨母细胞瘤。

Results: of 52 cases, a total of 33 intracranial aneurysms were detected by the application of volume viewer, which clearly displayed the body, neck and parent artery of intracranial aneurysms, and showed the aneurysms relation with peripheral vessels and cranioaural In 52cases, 8 can of cerebral vascular malformations showed the form, the size and the hemisphere of cacogenesis cerebral vessels, as well a the amount of feeding artery and draining vein.

结果：52例中，脑动脉瘤33例，16层CT容积成像清晰显示所有动脉瘤的形态、大小、生长方向、瘤颈特征及其与载瘤动脉的关系、瘤体与相邻血管和颅骨的三维结构关系，优于DSA脑血管畸形8例，16层CT容积成像并显示畸形血管团的形态、范围大小以及供血动脉、引流静脉的形态及数量。

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时，他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了