痘苗源

Heteroduplex molecules have been made by mixing pJSA1175 and pJSAl175ADRV4, pJSA1175ADRV4 and pJSAl175RRV4 DNA molecules which have been linearized by Pst Ⅰ and Bgl Ⅱ respectively, denaturing them in alkaline or high temperature, allowing them to renature and then spreading in the presence of formamide.

以痘苗病毒表达载体pJSA1175及重组质粒pJSA1175ADRV4(含ADRV VP4基因)、pJSA1175RRV4(含猴轮状病毒VP4基因)为材料，用碱变性方法或热变性方法制备了载体质粒与重组质粒DNA之间的异源双链，电镜下成功观察到载体序列部分经变性、复性后配对呈双链状态，而外源基因区域突出呈单链环状结构，测量长度为2.3kb。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。