痘苗

In the last part of thesis, primary study of enhancer binding protein was done using yeast one-hybrid system.

在本文的最后一部分，首次应用酵母单杂交系统对痘苗病毒增强子序列在大肠杆菌中的DNA结合蛋白作了初步研究。

The therapeutic effect of recombinant vaccinia virus encoding human IL-2 gene(IL-2VV) on the tumor-bearing mice was investigated. The tumor-bearing mice were established by subcutaneus inoculation with 2×106 FBL-3 erythroblastic leukemia cells into C57BL/6 mice.

观察了编码人白细胞介素2(IL-2)基因的重组痘苗病毒(recombinant vaccinia virus encoding human IL-2 gene，IL-2VV)对小鼠接种FBL-3红白血病细胞诱发肿瘤的治疗作用。

Heteroduplex molecules have been made by mixing pJSA1175 and pJSAl175ADRV4, pJSA1175ADRV4 and pJSAl175RRV4 DNA molecules which have been linearized by Pst Ⅰ and Bgl Ⅱ respectively, denaturing them in alkaline or high temperature, allowing them to renature and then spreading in the presence of formamide.

以痘苗病毒表达载体pJSA1175及重组质粒pJSA1175ADRV4(含ADRV VP4基因)、pJSA1175RRV4(含猴轮状病毒VP4基因)为材料，用碱变性方法或热变性方法制备了载体质粒与重组质粒DNA之间的异源双链，电镜下成功观察到载体序列部分经变性、复性后配对呈双链状态，而外源基因区域突出呈单链环状结构，测量长度为2.3kb。

Freshly isolated blood mononuclear cells were reversely penetrated by unstimulated human umbilical vein endothelial cells and cultured in medium containing GM-CSF, IL-4 and TNF- a .

此外，本实验还用重组人CEA痘苗病毒转染DC，构建DC疫苗，以求在CEA阳性肿瘤患者的生物治疗上有所突破。

Objective:To study the preventive effect of CEA recombinant vaccina viruson CEA positive tumor.

目的：探讨CEA－重组痘苗病毒对CEA阳性肿瘤的预防作用。

Both the recombinant vaccina viruses expressing the mutated or wild E6 protein could induce the specific antibobies, but neither of them could produce CTL and immune protection.

表达野生型和突变型E6的重组痘苗病毒可诱发C57BL／6小鼠产生特异性抗体，但都未能诱发CTL反应，在抗肿瘤移植实验中也未显示出免疫保护作用。

In order to test the antibodies to E6 and E7 induced by the recombinant vaccina virus, the fusion proteins of GST and E6 or E7 were expressed in E. coli using pGEX-2T vector. The expressed proteins were confirmed by Western-blot and purified by glutathione sepharose CL-4B affinity chromatography. The purified proteins were used to detect the anti-E6 and-E7 by ELISA.

为便于检测重组痘苗病毒所诱发的E6和E7抗体，本文利用原核表达载体pGEX-2T，在大肠杆菌中分别高效表达了GST与E6或E7的融合蛋白，并利用纯化的融合蛋白成功地建立了E6和E7抗体的ELISA检测方法。

Efficiency: Bactericidal, Virucidal (incl. HBV,HCV,HIV,SARS,H5N1,Vaccinia,virua), and fungicidal100g 中含 Isopropanol 62.80g;Aqua bidest37.20g, Perfume and colorant free

生效范围：细菌，病毒（包括乙肝病毒，丙型肝炎病毒，爱滋病毒，SARS病毒，禽流感病毒，痘苗病毒）和真菌

It is the beginning for the recombinant to express protien product in eukaryotic cell.

HPV16 E7基因痘苗病毒重组体构建成功，为其在真核细胞的表达奠定了基础。

After inserting the mutant fluorescence protein gene into the vector, the novel vaccinia virus vector p16H1FP and p16H2FP to express proteins as fusion products containing an additional amino-terminal stretch of six histidines were constructed.

我们用PCR扩出N-端融合6×His·Tag多克隆位点基因序列，插入p16表达载体，并在其多克隆位点下游装入带有巨细胞病毒启动子的突变型荧光蛋白基因，构建成两个读框的N-端融合6×His·Tag并带有荧光蛋白报告基因的分离型痘苗病毒表达载体。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新